Advertisement

Processing Harvested Fat

  • Mario Goisis
  • Sara Izzo
  • Andrea Sbarbati
  • Giamaica Conti
  • Giovanni Francesco Nicoletti
Chapter

Abstract

As it is the case of outpatient procedures, there are different methods by which to process fat. These methods produce different results, in particular those regarding the four most important aspects of the long-term durability of a graft.

References

  1. 1.
    Boschert MT, Beckert BW, Puckett CL, Concannon MJ. Analysis of lipocyte viability after liposuction. Plast Reconstr Surg. 2002;109:761–5.CrossRefGoogle Scholar
  2. 2.
    Peer LA. Cell survival theory versus replacement theory. Plast Reconstr Surg (1946). 1955;16:161–8.CrossRefGoogle Scholar
  3. 3.
    Eto H, Kato H, Suga H, Aoi N, Doi K, Kuno S, Yoshi- mura K. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adi- pocytes. Plast Reconstr Surg. 2012;129:1081–92.CrossRefGoogle Scholar
  4. 4.
    Zuk PA, Zhu M, Ashjian P, et al. Human adipose tis- sue is a source of multipotent stem cells. Mol Biol Cell. 2002;13:4279–95.CrossRefGoogle Scholar
  5. 5.
    Kølle SF, Fischer-Nielsen A, Mathiasen AB, et al. Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue- derived stem cells for graft survival: a randomised placebo- controlled trial. Lancet. 2013;382:1113–20.CrossRefGoogle Scholar
  6. 6.
    Zhu M, Cohen SR, Hicok KC, Shanahan RK, Strem BM, Yu JC, Arm DM, Fraser JK. Comparison of three different fat graft preparation methods: gravity separation, centrifugation, and simultaneous washing with filtration in a closed system. Plast Reconstr Surg. 2013;131(4):873–80.CrossRefGoogle Scholar
  7. 7.
    Cleveland EC, Albano NJ, Hazen A. Roll, spin, wash, or filter? Processing of lipoaspirate for autologous fat grafting: an updated, evidence-based review of the literature. Plast Reconstr Surg. 2015;136(4):706–13.CrossRefGoogle Scholar
  8. 8.
    Botti G, Pascali M, Botti C, Bodog F, Cervelli V. A clinical trial in facial fat grafting: filtered and washed versus centrifuged fat. Plast Reconstr Surg. 2011;127:2464–73.CrossRefGoogle Scholar
  9. 9.
    Gir P, Brown SA, Oni G, Kashefi N, Mojallal A, Rohrich RJ. Fat grafting: evidence-based review on autologous fat harvesting, processing, reinjection, and storage. Plast Reconstr Surg. 2012;130:249–58.CrossRefGoogle Scholar
  10. 10.
    Hoareau L, Bencharif K, Girard AC, et al. Effect of centrifugation and washing on adipose graft viability: a new method to improve graft efficiency. J Plast Reconstr Aesthet Surg. 2013;66:712–9.CrossRefGoogle Scholar
  11. 11.
    Condé-Green A, Wu I, Graham I, et al. Comparison of 3 techniques of fat grafting and cell-supplemented lipotransfer in athymic rats: a pilot study. Aesthet Surg J. 2013;33:713–21.CrossRefGoogle Scholar
  12. 12.
    Pu LL, Coleman SR, Cui X, Ferguson RE Jr, Vasconez HC. Autologous fat grafts harvested and refined by the Coleman technique: a comparative study. Plast Reconstr Surg. 2008;122:932–7.CrossRefGoogle Scholar
  13. 13.
    Conde-Green A, Gontijo de Amorim N, Pitanguy I. Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: a comparative study. J Plast Reconstr Aesthet Surg. 2010;63:1375–81.CrossRefGoogle Scholar
  14. 14.
    Pfaff M, Wu W, Zellner E, Steinbacher DM. Processing technique for lipofilling influences adipose-derived stem cell concentration and cell viability in lipoaspirate. Aesthet Plast Surg. 2014;38:224–9.CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Mario Goisis
    • 1
  • Sara Izzo
    • 2
  • Andrea Sbarbati
    • 3
  • Giamaica Conti
    • 3
  • Giovanni Francesco Nicoletti
    • 2
  1. 1.Maxillo-Facial and Aesthetic SurgeonGo Easy ClinicMilanItaly
  2. 2.Department of Plastic SurgeryUniversity of Campania “Luigi Vanvitelli”NaplesItaly
  3. 3.Department of Neuroscience, Biomedicine and Movement, Human Anatomy and Histology SectionUniversity of VeronaVeronaItaly

Personalised recommendations