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Nonlinear Optical Characterization of Membrane Protein Microcrystals and Nanocrystals

  • Justin A. Newman
  • Garth J. Simpson
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 922)

Abstract

Nonlinear optical methods such as second harmonic generation (SHG) and two-photon excited UV fluorescence (TPE-UVF) imaging are promising approaches to address bottlenecks in the membrane protein structure determination pipeline. The general principles of SHG and TPE-UVF are discussed here along with instrument design considerations. Comparisons to conventional methods in high throughput crystallization condition screening and crystal quality assessment prior to X-ray diffraction are also discussed.

Keywords

Nonlinear optical microscopy Second harmonic generation Two-photon excited UV fluorescence Microscopy High-throughput screening Crystal detection Crystal positioning Crystal optimisation 
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Notes

Acknowledgments

The authors wish to acknowledge support from the National Institutes of Health (NIH) grants NIH-R01GM103401 and NIH-R01GM103910. We would also like to thank Ellen Gualtieri from Formulatrix for supplying the glucose isomerase crystal images in Fig. 7.2.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Department of ChemistryPurdue UniversityWest LafayetteUSA

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