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Nonlinear Optical Characterization of Membrane Protein Microcrystals and Nanocrystals

  • Justin A. Newman
  • Garth J. SimpsonEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 922)

Abstract

Nonlinear optical methods such as second harmonic generation (SHG) and two-photon excited UV fluorescence (TPE-UVF) imaging are promising approaches to address bottlenecks in the membrane protein structure determination pipeline. The general principles of SHG and TPE-UVF are discussed here along with instrument design considerations. Comparisons to conventional methods in high throughput crystallization condition screening and crystal quality assessment prior to X-ray diffraction are also discussed.

Keywords

Nonlinear optical microscopy Second harmonic generation Two-photon excited UV fluorescence Microscopy High-throughput screening Crystal detection Crystal positioning Crystal optimisation 
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Notes

Acknowledgments

The authors wish to acknowledge support from the National Institutes of Health (NIH) grants NIH-R01GM103401 and NIH-R01GM103910. We would also like to thank Ellen Gualtieri from Formulatrix for supplying the glucose isomerase crystal images in Fig. 7.2.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Department of ChemistryPurdue UniversityWest LafayetteUSA

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