Autofluorescence Imaging

  • Massimiliano di Pietro
  • Krish RagunathEmail author


With the aim to reduce cancer mortality, early detection of neoplasia is one of the most challenging tasks in upper and lower of gastrointestinal endoscopy. Despite microscopic architectural and cellular differences between normal, premalignant and early malignant epithelia, standard endoscopy often fails to be sufficiently informative for the human eye, due to the lack of colour contrast in white-light mode. Autofluorescence imaging (AFI) is based on the property of short wavelengths to excite endogenous molecules in the gastrointestinal mucosa to emit fluorescence and exploits different fluorescent properties of neoplastic compared to normal tissue. Hence, AFI stands as a red-flag wide-field endoscopic technique for early cancer detection.


Autofluorescence Endoscopy Spectroscopy Early cancer Dysplasia Barrett’s oesophagus Squamous cell carcinoma Adenoma Gastric atrophy Ulcerative colitis 

Supplementary material

Video 5.1

Trimodal imaging demonstrating an early cancer in a short tongue of Barrett’s oesophagus (MP4 3309 kb)

Video 5.2

Trimodal imaging demonstrating a flat adenoma in the sigmoid colon (MP4 6093 kb)


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.MRC Cancer Unit, University of Cambridge, Cambridge Biomedical CampusCambridgeUK
  2. 2.NIHR Biomedical Research Unit in Gastrointestinal and Liver Diseases at Nottingham University Hospitals NHS Trust and the University of Nottingham, Queens Medical Centre CampusNottinghamUK

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