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Carbohydrate Analysis with Capillary Electrophoresis

  • Antje Klockow-Beck
  • Aran Paulus
Part of the BioMethods book series (BIOMETHODS)

Abstract

Capillary electrophoresis (CE) is an automated, high-resolution, miniaturized separation technique. CE instrumentation is similar to high-pressure liquid chromatography (HPLC) and allows unattended operation with on-column detection and computer-aided data processing. Typically, several hundred thousand theoretical plates per column can be achieved in run times of less than 20 min. The separation is accomplished in fused-silica capillaries of 50–100 µm inner diameter (ID) and 20–50 cm in length. The application of a high electrical field in the order of several hundred V/cm induces a differential migration of charged solutes, making CE an attractive separation tool, especially for biological samples.

Keywords

Acetic Anhydride Coated Capillary Complex Oligosaccharide Borate Concentration Trisulfonic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag Basel 1997

Authors and Affiliations

  • Antje Klockow-Beck
  • Aran Paulus

There are no affiliations available

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