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Mass Spectrometric Analysis of Highly Acidic Polysaccharides

  • Andrew J. Rhomberg
  • Klaus Biemann
Part of the BioMethods book series (BIOMETHODS)

Abstract

Glycosaminoglycans (GAGs) are a class of highly acidic polysaccharides that are found in the extracellular matrix and in proteoglycans, which are cell membrane-bound proteins that are post-translationally modified with GAGs (1, 2). Analysis of these compounds by classical methods has been difficult due to the heterogeneous and polyanionic nature of GAGs. However, by mixing GAG oligosaccharides with basic peptides to form a noncovalent complex, it is possible to accurately determine their molecular weight using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) (3, 4). Such molecular weight measurements provide information on the length of the carbohydrate chain and the degree of sulfation. In addition, mass spectrometric measurements on enzymatic and chemical degradation products of isolated compounds may yield the entire structure of the analyte of interest. Figure 5.1 represents the structure of a heparin fragment.

Keywords

Caffeic Acid Noncovalent Complex Acidic Polysaccharide Lower Laser Intensity Basic Peptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag Basel 1997

Authors and Affiliations

  • Andrew J. Rhomberg
  • Klaus Biemann

There are no affiliations available

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