HPAE-PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride Membranes

  • Michael Weitzhandler
  • Nebojsa Avdalovic
Part of the BioMethods book series (BIOMETHODS)


Protein glycosylation is a major post-translational modification that generates a potentially large group of glycoforms from a single polypeptide chain (1). Over 50% of catalogued protein sequences contain the requisite sequence for N-glycosylation (AsnXxxSer(Thr); Xxx ≠ Pro) (2), and any Ser or Thr can potentially be glycosylated (3). Since glycosylation can play an important role in the biological and pharmacologic activity of the glycoprotein, there is increasing interest in identifying and characterizing the oligosaccharide moieties of glycoproteins.


Sialic Acid Amino Sugar Carbohydrate Analysis Monosaccharide Analysis Membrane Band 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Birkhäuser Verlag Basel 1997

Authors and Affiliations

  • Michael Weitzhandler
  • Nebojsa Avdalovic

There are no affiliations available

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