Analysis of Protein — Carbohydrate Interactions by FACE/Gel Retardation Assay

  • Guo-fu Hu
Part of the BioMethods book series (BIOMETHODS)


Binding of carbohydrates to proteins is an important feature of a wide variety of biological systems that often has physiological and pathological significance. For example, interactions between lectins and carbohydrates are critical for the initiation of viral, bacterial, mycoplasmal, and parasitic infections; cell-cell adhesion; fertilization and development; cell proliferation and metastasis (1). Binding of selectins, a class of carbohydrate-binding proteins located on the endothelial cell surface, to leukocyte carbohydrates has been demonstrated to be an essential step in the trafficking and recruitment of leukocytes to injured tissues (2). Moreover, cellular receptors for many growth factors are glycoproteins, and the carbohydrate moieties participate in binding and signal transduction to the cells.


Face Imager Scatchard Plot Glucose Polymer Apparent Dissociation Constant Sodium Cyanoborohydride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Williams B A, Chervenak MC and Toone EJ (1992) J. Biol Chem. 267: 2 2907.Google Scholar
  2. 2.
    McEver RP, Moore KL and Cummings RD (1995) J. Biol Chem. 270: 1 1025.Google Scholar
  3. 3.
    Hu G-F and Vallee BL (1994) Anal Biochem. 218: 185.PubMedCrossRefGoogle Scholar
  4. 4.
    Jackson P (1990) Biochem. J. 270: 705.PubMedGoogle Scholar
  5. 5.
    Patel T, Bruce J, Merry A, Bigge C, Wormald M, Jaques A and Parekh R (1993) Biochemistry 32: 679.PubMedCrossRefGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel 1997

Authors and Affiliations

  • Guo-fu Hu

There are no affiliations available

Personalised recommendations