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Exosequencing of Heparan Sulphate/Heparin Saccharides Using Lysosomal Enzymes

  • Jeremy E. Turnbull
  • John J. Hopwood
  • John T. Gallagher
Part of the BioMethods book series (BIOMETHODS)

Abstract

Heparan sulphates (HS) are common components of mammalian cell surfaces and the extracellular matrix. They are normally present in the form of proteoglycans with the HS chains covalently linked to a restricted number of membrane-associated or secretory proteins. Heparan sulphates have diverse biological functions which include the maintenance of tissue architecture, the control of blood fluidity at the endothelial surface and the regulation of cell growth and morphogenesis. Many of these regulatory functions can be reproduced by the chemically related heparin, a product of connective tissue mast cells, which is widely used as an anticoagulant drug and which may eventually be used in therapy of cell growth-related diseases such as cancer and atherosclerosis.

Keywords

Heparan Sulphate High Pressure Liquid Chromatography Desalt Column Heparan Sulphate Chain Ammonium Persulphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag Basel 1997

Authors and Affiliations

  • Jeremy E. Turnbull
  • John J. Hopwood
  • John T. Gallagher

There are no affiliations available

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