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HPLC Strategies for Profiling and Sequencing Oligosaccharides

  • Geoffrey R. Guile
  • Pauline M. Rudd
  • David R. Wing
  • Raymond A. Dwek
Part of the BioMethods book series (BIOMETHODS)

Abstract

The sugars released from a pure glycoprotein often consist of a heterogeneous population containing both neutral and charged oligosaccharides. For example, the single N-glycosylation site in human erythrocyte CD59 is associated with more than 100 neutral and sialylated complex glycans, each representing a different glycoform (1). The existence of such extensive heterogeneity in biologically important glycoproteins requires refined approaches to the analysis of oligosaccharides. The adaptable technology which is described here represents a significant advance towards faster, more automated and more detailed strategies for the rapid profiling and analysis of sugars. Such technologies may be required for major studies, such as the human genome project, which defines DNA in normal and diseased states, and the proteome project, which sets out to analyse the total amount of protein in a living cell. It is worthy of note that genetic diseases are not caused by the genes themselves, but by the products for which the genes code and their post-translational modifications, which include glycosylation. In this chapter two strategies for rapid oligosaccharide analysis are described: Oligosaccharide profiling and detailed structural analysis.

Keywords

Sialic Acid Normal Phase Glucose Unit Jack Bean Normal Phase HPLC 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag Basel 1997

Authors and Affiliations

  • Geoffrey R. Guile
  • Pauline M. Rudd
  • David R. Wing
  • Raymond A. Dwek

There are no affiliations available

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