Improvement of the Model System to Develop Eco-Friendly Bio-Utilization of Phosphogypsum
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This paper focused on the modeling of the possibility of bacteria growth under medium that different content phosphogypsum (PG) doses for environmental protection purposes with special attention to the analysis of the effect of PG features underestimation of E. coli growth. The culture of E. coli is diluted with Lysogeny broth (LB) initially without adding PG to obtain an optical density at 600 nm (OD600) of 0.05. Study is carried out by adding different doses of PG (250 mg/200 mL LB; 500 mg/200 mL LB; 1000 mg/200 mL LB). The OD600 is measured with the use of an absorption spectrophotometer. Under modeling PG feature effluence, several factors are identified that impact on bacteria growth and the general methodological approach to assessing the biochemical activity of PG is formed. The important direction for feature study the effect of PG use as a component of the medium for E. coli is the assessment of mutations and adaptive biochemical mechanisms, in particular, the possibility of biofilm formation. Microorganisms in biofilms are better adapted and much more resistant to high concentrations of various groups of xenobiotics. In some cases, the matrix itself is involved in bioremediation, sorbing and retaining toxic substances from the aqueous phase.
KeywordsEnvironmental protection Phosphogypsum Modeling influence E. coli Bioremediation
We thank Prof. Leonid Plyatsuk (Sumy State University) for reading the manuscript and helpful discussion and Takahiro Hamaguchi (Chubu University) for his technical support. This work has been supported by the fellowship program of the Matsumae International Foundation (to YC), and the research fund from Chubu University (to KH).
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