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Single Compartment Approach for Assembling Photosynthetic Protocells

  • Emiliano Altamura
  • Paola Albanese
  • Francesco Milano
  • Massimo Trotta
  • Pasquale Stano
  • Fabio MavelliEmail author
Conference paper
  • 52 Downloads
Part of the Lecture Notes in Bioengineering book series (LNBE)

Abstract

In this work, we show the extraction, purification and reconstitution of the bacterial protein complex bc1 in giant lipid vesicles used as versatile micro-sized enzymatic reactors. Firstly, it is demonstrated that the bc1 shows an enzymatic activity also as micellar suspension when the proper substrates are added. Moreover, it has been possible to reconstitute, with the droplet transfer method, the bc1 complex in artificial giant lipid vesicles. The reconstitution has been proved by confocal microscopy analysis demonstrating a change of internal pH, thanks to a pH-sensitive fluorescent dye, upon the addition of a trigger substrate.

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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Emiliano Altamura
    • 1
  • Paola Albanese
    • 1
  • Francesco Milano
    • 2
  • Massimo Trotta
    • 3
  • Pasquale Stano
    • 4
  • Fabio Mavelli
    • 1
    • 5
    Email author
  1. 1.Department of ChemistryUniversity of Bari Aldo MoroBariItaly
  2. 2.CNR-ISPA, Institute of Sciences of Food Production, Lecce UnitLecceItaly
  3. 3.CNR-IPCF, Institute for Physical and Chemical ProcessesBariItaly
  4. 4.Department of Biological and Environmental Sciences and TechnologiesUniversity of SalentoLecceItaly
  5. 5.Institute of Nanotechnology, CNR NANOTECBariItaly

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