Transport, Cryopreservation and Storage of Ovarian Tissue

  • Jana LiebenthronEmail author


Cryopreservation of ovarian tissue using the slow freezing method is an established fertility preservation procedure in post-pubertal patients. In comparison to cryopreservation of oocytes, only a few days are required, and therefore gonadotoxic treatment does not necessarily need to be postponed. In diseases with a low risk of ovarian metastasis, the efficacy has been proven with a cumulative live birth of approximately 30–35%. Experience with this technique is still very limited in pre-pubertal patients.


Centralized cryobanking Fertility preservation FertiPROTEKT Gonadotoxic therapy Ovarian tissue cryopreservation Overnight transportation Slow freezing 


  1. 1.
    Schmidt KL, Ernst E, Byskov AG, Nyboe AA, Yding AC. Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation. Hum Reprod. 2003;18(12):2654–9. Scholar
  2. 2.
    Liebenthron J, Montag M, Reinsberg J, Köster M, Isachenko V, van der Ven K, et al. Overnight ovarian tissue transportation for centralized cryobanking: a feasible option. Reprod Biomed Online. 2019;38(5):740–9. Epub 2019 Jan 19CrossRefPubMedGoogle Scholar
  3. 3.
    von Wolff M, Andersen CY, Woodruff TK, Nawroth F. FertiPROTEKT, Oncofertility consortium and the Danish fertility-preservation networks - what can we learn from their experiences? Clin Med Insights Reprod Health. 2019;13:1179558119845865. eCollection 2019CrossRefGoogle Scholar
  4. 4.
    Rosendahl M, Schmidt KT, Ernst E, Rasmussen PE, Loft A, Byskov AG, et al. Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique. Reprod Biomed Online. 2011;22(2):162–71. Epub 2010 Nov 16CrossRefPubMedGoogle Scholar
  5. 5.
    Gellert SE, Pors SE, Kristensen SG, Bay-Bjørn AM, Ernst E, Yding AC. Transplantation of frozen-thawed ovarian tissue: an update on worldwide activity published in peer-reviewed papers and on the Danish cohort. J Assist Reprod Genet. 2018;35(4):561–70. Epub 2018 Mar 1CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Donnez J, Dolmans MM. Ovarian tissue freezing: current status. Curr Opin Obstet Gynecol. 2015;27(3):222–30. ReviewCrossRefPubMedGoogle Scholar
  7. 7.
    Bastings L, Liebenthron J, Westphal JR, Beerendonk CC, van der Ven H, Meinecke B, et al. Efficacy of ovarian tissue cryopreservation in a major European center. J Assist Reprod Genet. 2014;31(8):1003–12. Epub 2014 Jun 14CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Beckmann MW, Lotz L, Toth B, Baston-Büst DM, Fehm T, Frambach T, et al. Concept paper on the technique of cryopreservation, removal and transplantation of ovarian tissue for fertility preservation. Geburtshilfe Frauenheilkd. 2019;79(1):53–62. Epub 2018 Oct 9CrossRefPubMedGoogle Scholar
  9. 9.
    Deutsche Gesellschaft für Gynäkologie und Geburtshilfe (DGGG), Deutsche Gesellschaft für Reproduktionsmedizin (DGRM), Deutsche Gesellschaft für Urologie (DGU). Leitlinie: Fertilitätserhaltung bei onkologischen Therapien. Level S2k, AWMF Register Nr. 015/082, November 2017.
  10. 10.
    Gosden RG, Baird DT, Wade JC, Webb R. Restoration of fertility to oophorectomized sheep by ovarian autografts stored at −196°C. Hum Reprod. 1994;9:597–603. Scholar
  11. 11.
    Liebenthron J, Reinsberg J, van der Ven H, Saenger N, Kruessel JS, von Wolff M. Serum anti-Mullerian hormone concentration and follicle density throughout reproductive life and in different diseases - implications in fertility preservation. Hum Reprod. 2019;34(12):2513–22. Scholar
  12. 12.
    Isachenko E, Isachenko V, Nawroth F, Rahimi G, Weiss JM. Effect of long-term exposure at suprazero temperatures on activity and viability of human ovarian cortex. Fertil Steril. 2009;91(4 Suppl):1556–9. Epub 2008 Nov 20CrossRefPubMedGoogle Scholar
  13. 13.
    Mortimer D, Cohen J, Mortimer ST, Fawzy M, McCulloh DH, Morbeck DE, et al. Cairo consensus on the IVF laboratory environment and air quality: report of an expert meeting. Reprod Biomed Online. 2018;36(6):658–74. Epub 2018 Mar 2CrossRefPubMedGoogle Scholar

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© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.UniCareD, University Cryobank for Assisted Reproductive Medicine and Fertility Protection at UniKiD Duesseldorf, University Hospital DuesseldorfDuesseldorfGermany

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