Prompt and Convenient Preparation of Oral Vaccines Using Yeast Cell Surface Display
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The budding yeast, Saccharomyces cerevisiae, has been widely used in fermentation technology. In recent, yeast cell surface has been investigated as potential spaces for immobilizing molecules of interest. The technology of yeast cells to display foreign proteins or peptides by genetic manipulation has been called “cell surface engineering (arming technology)” or “molecular display technology.” Here, the yeast molecular display system is described as convenient method to produce an oral vaccine against various infectious diseases. An antigen derived from Candida albicans was successfully displayed, and it was evaluated on an effect in oral vaccination. Additionally, to expand a possibility of the yeast molecular display technology, screening approach on antigens using proteomic analyses is introduced. Useful combination of proteomic analysis and molecular display technology is discussed with practical application.
KeywordsSaccharomyces cerevisiae Cell surface Vaccine Candida albicans
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