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Functional and Bioactive Properties of Hemp Proteins

  • Tamara Dapčević-HadnađevEmail author
  • Miroslav Hadnađev
  • Manda Dizdar
  • Nataša Jovanović Lješković
Chapter
  • 33 Downloads
Part of the Sustainable Agriculture Reviews book series (SARV, volume 42)

Abstract

Hemp seeds primarily represent a source of edible oil, which comprises over 30% of the whole seed. However, the role of the hemp seed, as a valuable material for protein extraction, could not be neglected. While the hemp seed is characterized with the protein content of 25%, after oil extraction, the protein content in a hempseed cake, a by-product of the oil extraction process, can increase up to 50%. Hempseed protein mainly consists of a legumin type protein, edestin, which accounts for 60–80% of the total protein content, followed by albumin. Recently, the ability of hemp protein to act as a techno-functional agent in different food applications has been investigated. The role of hemp protein as emulsifiers, foaming agent, gel-forming and biodegradable film-forming material was studied pointing the possibility to replace synthetic agents with the natural ones. Moreover, a large number of studies have revealed a bio-functionality of hemp proteins, i.e. application of enzymatic hydrolysis for the production of bioactive peptides. Bioactivity was mostly investigated by determining antioxidant properties and antihypertensive effects of enzymatic hemp seed protein hydrolysates and their peptide fractions. The hydrolysis was achieved by employing a range of proteases as well as different degrees of hydrolysis, which resulted in significant differences in the antioxidant properties of obtained hemp protein hydrolysates. The present chapter is a review of recent information on hemp protein extraction techniques, with the special emphases to its techno- and bio-functionality.

Keywords

Hemp protein Isolation technique Hydration Gelling Interfacial properties Bioactive peptides 

Abbreviations

ACE

angiotensin-I-converting enzyme

DPPH

2,2-diphenyl-1-picrylhydrazyl

DPPH

2,2-diphenyl-1-picrylhydrazyl radical

DSC

differential scanning calorimetry

HPH

hemp protein hydrolysate

HPI

hemp protein isolate

IEP

alkaline extraction-isoelectric precipitation

MW

molecular weight

OHC

oil holding properties

pI

isoelectric point

S

Svedberg unit

WHC

water holding properties

Notes

Acknowledgements

This work was financially supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia under the Agreement on the Implementation and Financing of Research of the Institute of Food Technology (No. 451-03-68/2020-14/200222) and the Provincial Secretariat for Higher Education and Scientific Research, Republic of Serbia [grant number 142-451-2138/2019-01/02] as a part of the project entitled “Techno-functionality of proteins from alternative plant sources from Vojvodina region”.

References

  1. Aiello G, Fasoli E, Boschin G, Lammi C, Zanoni C, Citterio A, Arnoldi A (2016) Proteomic characterization of hempseed (Cannabis sativa L.). J Proteome 147:187–196.  https://doi.org/10.1016/j.jprot.2016.05.033 CrossRefGoogle Scholar
  2. Alsohaimy SA, Sitohy MZ, El-Masry RA (2007) Isolation and partial characterization of chickpea, lupine and lentil seed proteins. World J Agric Sci 3:123–129. ISSN:1817-3047Google Scholar
  3. Amarowicz R (2010) Modification of emulsifying properties of food proteins by enzymatic hydrolysis. Eur J Lipid Sci Technol 112:695–696.  https://doi.org/10.1002/ejlt.201000382 CrossRefGoogle Scholar
  4. Arntfield SD, Ismond MA, Murray ED (1985) The fate of antinutritional factors during the preparation of a fababean protein isolate using a micellization technique. Can Inst Food Sci Technol J 18:137–143.  https://doi.org/10.1016/S0315-5463(85)71771-3 CrossRefGoogle Scholar
  5. Battin EE, Brumaghim JL (2009) Antioxidant activity of sulfur and selenium: a review of reactive oxygen species scavenging, glutathione peroxidase, and metal-binding antioxidant mechanisms. Cell Biochem Biophys 55:1–23.  https://doi.org/10.1007/s12013-009-9054-7 CrossRefPubMedGoogle Scholar
  6. Bourtoom T (2008) Edible films and coatings: characteristics and properties. Int Food Res J 15:237–248Google Scholar
  7. Boye JI, Akshay S, Ribéreau S, Mondor M, Farnworth E, Rajamohamed SH (2010) Comparison of the functional properties of pea, chickpea and lentil protein concentrates processed using ultrafiltration and isoelectric precipitation techniques. Food Res Int 43:537–546.  https://doi.org/10.1016/j.foodres.2009.07.021 CrossRefGoogle Scholar
  8. Caetano da Silva Lannes S, Natali Miquelim J (2013) Interfacial behavior of food proteins. Curr Nutr Food Sci 9:10–14.  https://doi.org/10.2174/157340113804810914 CrossRefGoogle Scholar
  9. Callaway JC (2004) Hempseed as a nutritional resource: an overview. Euphytica 140:65–72.  https://doi.org/10.1007/s10681-004-4811-6 CrossRefGoogle Scholar
  10. Chang CY, Wu KC, Chiang SH (2007) Antioxidant properties and protein compositions of porcine haemoglobin hydrolysates. Food Chem 100:1537–1543.  https://doi.org/10.1016/j.foodchem.2005.12.019 CrossRefGoogle Scholar
  11. Chen HM, Muramoto K, Yamauchi F (1995) Structural analysis of antioxidative peptides from soybean β-Conglycinin. J Agric Food Chem 43:574–578.  https://doi.org/10.1021/jf00051a004 CrossRefGoogle Scholar
  12. Dapčević-Hadnađev T, Hadnađev M, Lazaridou A, Moschakis T, Biliaderis CG (2019a) Hempseed meal protein isolates prepared by different isolation techniques. Part II. Gelation properties at different ionic strengths. Food Hydrocoll 81:481–489.  https://doi.org/10.1016/j.foodhyd.2018.03.022 CrossRefGoogle Scholar
  13. Dapčević-Hadnađev T, Dizdar M, Pojić M, Krstonošić V, Zychowski LM, Hadnađev M (2019b) Emulsifying properties of hemp proteins: effect of isolation technique. Food Hydrocoll 89:912–920.  https://doi.org/10.1016/j.foodhyd.2018.12.002 CrossRefGoogle Scholar
  14. Erdmann K, Cheung BWY, Schroder H (2008) The possible roles of food derived bioactive peptides in reducing the risk of cardiovascular disease. J Nutr Biochem 19:643–654.  https://doi.org/10.1016/j.jnutbio.2007.11.010 CrossRefPubMedGoogle Scholar
  15. FitzGerald RJ, Meisel H (2000) Milk protein-derived peptide inhibitors of angiotensin-I-converting enzyme. Br J Nutr 8:33–37.  https://doi.org/10.1017/S0007114500002221 CrossRefGoogle Scholar
  16. FitzGerald RJ, Murray BA, Walsh DJ (2004) Hypotensive peptides from milk proteins. J Nutr 134:980–988.  https://doi.org/10.1093/jn/134.4.980S CrossRefGoogle Scholar
  17. Fredrikson M, Biot P, Alminger ML, Carlsson NG, Sandberg AS (2001) Production process for high quality pea protein isolate with low content of oligosaccharides and phytate. J Agric Food Chem 49:1208–1212.  https://doi.org/10.1021/jf000708x CrossRefPubMedGoogle Scholar
  18. Fuhrmeister H, Meuser F (2003) Impact of processing on functional properties of protein products from wrinkled peas. J Food Eng 56:119–129.  https://doi.org/10.1016/S0260-8774(02)00241-8 CrossRefGoogle Scholar
  19. Girgih AT, Udenigwe CC, Aluko RE (2011a) In vitro antioxidant properties of hemp seed (Cannabis sativa L.) protein hydrolysate fractions. J Am Oil Chem Soc 88:381–389.  https://doi.org/10.1007/s11746-010-1686-7 CrossRefGoogle Scholar
  20. Girgih AT, Udenigwe CC, Li H, Adebiyi AP, Aluko RE (2011b) Kinetics of enzyme inhibition and antihypertensive effects of hemp seed (Cannabis sativa L.) protein hydrolysates. J Am Oil Chem Soc 88:1767–1774.  https://doi.org/10.1007/s11746-011-1841-9 CrossRefGoogle Scholar
  21. Girgih AT, Udenigwe CC, Aluko RE (2013) Reverse-phase HPLC separation of hemp seed (Cannabis sativa L.) protein hydrolysate produced peptide fractions with enhanced antioxidant capacity. Plant Foods Hum Nutr 68:39–46.  https://doi.org/10.1007/s11130-013-0340-6 CrossRefPubMedGoogle Scholar
  22. Girgih AT, Alashi AM, He R, Malomo SA, Aluko RE (2014) Preventive and treatment effects of hemp seed (Cannabis sativa L.) meal protein hydrolysate against high blood pressure in spontaneously hypertensive rats. Eur J Nutr 53:1237–1246.  https://doi.org/10.1007/s00394-013-0625-4 CrossRefPubMedGoogle Scholar
  23. Gómez-Guillén MC, Giménez B, López-Caballero MA, Montero MP (2011) Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocoll 25:1813–1827.  https://doi.org/10.1016/j.foodhyd.2011.02.007 CrossRefGoogle Scholar
  24. Hadnađev M, Hadnađev-Dapčević T, Pojić M, Šarić B, Mišan A, Jovanov P, Sakač M (2017) Progress in vegetable proteins isolation techniques: a review. Food Feed Res 44:11–21.  https://doi.org/10.5937/FFR1701011H CrossRefGoogle Scholar
  25. Hadnađev M, Dapčević-Hadnađev T, Lazaridou A, Moschakis T, Michaelidou AM, Popović S, Biliaderis CG (2018a) Hempseed meal protein isolates prepared by different isolation techniques. Part I. Physicochemical properties. Food Hydrocoll 79:526–533.  https://doi.org/10.1016/j.foodhyd.2017.12.015 CrossRefGoogle Scholar
  26. Hadnađev M, Dizdar M, Dapčević Hadnađev T, Jovanov P, Mišan A, Sakač M (2018b) Hydrolyzed hemp seed proteins as bioactive peptides. J Process Energy Agric 22:90–94.  https://doi.org/10.5937/JPEA1802090H CrossRefGoogle Scholar
  27. Hartmann R, Meisel H (2007) Food-derived peptides with biological activity: from research to food applications. Curr Opin Biotechnol 18:163–169.  https://doi.org/10.1016/j.copbio.2007.01.013 CrossRefPubMedGoogle Scholar
  28. Hernandez-Ledesma B, Quiros A, Amigo L, Recio I (2007) Identification of bioactive peptides after digestion of human milk and infant formula with pepsin and pancreatin. Int Dairy J 17:42–49.  https://doi.org/10.1016/j.idairyj.2005.12.012 CrossRefGoogle Scholar
  29. House JD, Neufeld J, Leson G (2010) Evaluating the quality of protein from hemp seed (Cannabis sativa L.) products through the use of the protein digestibility-corrected amino acid score method. J Agric Food Chem 58:11801–11807.  https://doi.org/10.1021/jf102636b CrossRefPubMedGoogle Scholar
  30. Isinguzo G (2011) Physicochemical, functional and in vitro bioactive properties of hempseed (Cannabis sativa) protein isolates and hydrolysates. MSc theses, Faculty of Graduate Studies of the University of Manitoba, CanadaGoogle Scholar
  31. Karaca AC, Low N, Nickerson M (2011) Emulsifying properties of chickpea, faba bean, lentil and pea proteins produced by isoelectric precipitation and salt extraction. Food Res Int 44:2742–2750.  https://doi.org/10.1016/j.foodres.2011.06.012 CrossRefGoogle Scholar
  32. Kaushik P, Dowling K, McKnight S, Barrow CJ, Wang B, Adhikari B (2016) Preparation, characterization and functional properties of flax seed protein isolate. Food Chem 197:212–220.  https://doi.org/10.1016/j.foodchem.2015.09.106 CrossRefPubMedGoogle Scholar
  33. Kim JJ, Lee MY (2011) Isolation and characterization of edestin from Cheungsam hempseed. J Appl Biol Chem 54:84–88.  https://doi.org/10.3839/jabc.2011.015 CrossRefGoogle Scholar
  34. Kim SY, Je JY, Kim SK (2007) Purification and characterization of antioxidant peptide from hoki (Johnius belengerii) frame protein by gastrointestinal digestion. J Nutr Biochem 18:31–38.  https://doi.org/10.1016/j.jnutbio.2006.02.006 CrossRefPubMedGoogle Scholar
  35. Kiosseoglou V, Paraskevopoulou A (2011) Chapter 3: Functional and physicochemical properties of pulse proteins. In: Tiwari BK, Gowen A, Mckenna B (eds) Pulse foods processing: quality and nutritional applications. Academic, San Diego, pp 57–89CrossRefGoogle Scholar
  36. Klompong V, Benjakul S, Kantachote D, Shahidi F (2007) Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroidesleptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chem 102:1317–1327.  https://doi.org/10.1016/j.foodchem.2006.07.016 CrossRefGoogle Scholar
  37. Korhonen H, Pihlanto A (2006) Bioactive peptides: production and functionality. Int Dairy J 16:945–960.  https://doi.org/10.1016/j.idairyj.2005.10.012 CrossRefGoogle Scholar
  38. Korus J, Witczak M, Ziobro R, Juszczak L (2017) Hemp (Cannabis sativa subsp. sativa) flour and protein preparation as natural nutrients and structure forming agents in starch based gluten-free bread. Lebensm Wiss Technol 84:143–150.  https://doi.org/10.1016/j.lwt.2017.05.046 CrossRefGoogle Scholar
  39. Kunwar A, Priyadarsini KI (2011) Free radicals, oxidative stress and importance of antioxidants in human health. J Med Allied Sci 1:53–60. ISSN:2231-1696Google Scholar
  40. Lam RS, Nickerson MT (2013) Food proteins: a review on their emulsifying properties using a structure–function approach. Food Chem 141:975–984.  https://doi.org/10.1016/j.foodchem.2013.04.038 CrossRefPubMedGoogle Scholar
  41. Liu Y, Zhao G, Ren J, Zhao M, Yang B (2011) Effect of denaturation during extraction on the conformational and functional properties of peanut protein isolate. Innov Food Sci Emerg Technol 12:375–380.  https://doi.org/10.1016/j.ifset.2011.01.012 CrossRefGoogle Scholar
  42. López OP, Ordorica-Falomir C (1986) Production of safflower protein isolates: composition, yield and protein quality. J Sci Food Agric 37:1097–1103.  https://doi.org/10.1002/jsfa.2740371107 CrossRefGoogle Scholar
  43. Ly YP, Johnson LA, Jane J (1998) Chapter 6: Soy protein as biopolymer. In: Kaplan D (ed) Biopolymers from renewable resources. Springer, Berlin/Heidelberg, pp 144–176CrossRefGoogle Scholar
  44. Malomo SA, Aluko RE (2015a) A comparative study of the structural and functional properties of isolated hemp seed (Cannabis sativa L.) albumin and globulin fractions. Food Hydrocoll 43:743–752.  https://doi.org/10.1016/j.foodhyd.2014.08.001 CrossRefGoogle Scholar
  45. Malomo SA, Aluko RE (2015b) Conversion of a low protein hemp seed meal into a functional protein concentrate through enzymatic digestion of fibre coupled with membrane ultrafiltration. Innov Food Sci Emerg Technol 31:151–159.  https://doi.org/10.1016/j.ifset.2015.08.004 CrossRefGoogle Scholar
  46. Malomo SA, He R, Aluko RE (2014) Structural and functional properties of hemp seed protein products. J Food Sci 79:C1512–C1521.  https://doi.org/10.1111/1750-3841.12537 CrossRefPubMedGoogle Scholar
  47. Malomo S, Onuh J, Girgih A, Aluko R (2015) Structural and antihypertensive properties of enzymatic hemp seed protein hydrolysates. Nutrients 7:7616–7632.  https://doi.org/10.3390/nu7095358 CrossRefPubMedPubMedCentralGoogle Scholar
  48. Mondor M, Aksay S, Drolet H, Roufik S, Farnworth E, Boye JI (2009) Influence of processing on composition and antinutritional factors of chickpea protein concentrates produced by isoelectric precipitation and ultrafiltration. Innov Food Sci Emerg Technol 10:342–347.  https://doi.org/10.1016/j.ifset.2009.01.007 CrossRefGoogle Scholar
  49. Moure A, Domínguez H, Parajó JC (2006) Antioxidant properties of ultrafiltration-recovered soy protein fractions from industrial effluents and their hydrolysates. Process Biochem 41:447–456.  https://doi.org/10.1016/j.procbio.2005.05.014 CrossRefGoogle Scholar
  50. Murray ED, Myers CD, Barker LD (1979) U.S. Patent No. 4,169,090. U.S. Patent and Trademark Office, Washington, DCGoogle Scholar
  51. Murray ED, Arntfield SD, Ismond MA (1985) The influence of processing parameters on food protein functionality II. Factors affecting thermal properties as analyzed by differential scanning calorimetry. Can Inst Food Sci Technol J 18:158–162.  https://doi.org/10.1016/S0315-5463(85)71774-9 CrossRefGoogle Scholar
  52. Mwasaru MA, Muhammad K, Bakar J, Man YBC (1999) Effects of isolation technique and conditions on the extractability, physicochemical and functional properties of pigeonpea (Cajanus cajan) and cowpea (Vigna unguiculata) protein isolates. I. Physicochemical properties. Food Chem 67:435–443.  https://doi.org/10.1016/S0308-8146(99)00150-8 CrossRefGoogle Scholar
  53. Nagpal R, Behare P, Rana R, Kumar A, Kumar M, Arora S, Morotta F, Jain S, Yadav H (2011) Bioactive peptides derived from milk proteins and their health beneficial potentials: an update. Food Funct 2:18–27.  https://doi.org/10.1039/c0fo00016g CrossRefPubMedGoogle Scholar
  54. Papalamprou EM, Doxastakis GI, Biliaderis CG, Kiosseoglou V (2009) Influence on preparation methods on physicochemical and gelation properties of chickpea protein isolates. Food Hydrocoll 23:337–343.  https://doi.org/10.1016/j.foodhyd.2008.03.006 CrossRefGoogle Scholar
  55. Papalamprou EM, Doxastakis GI, Kiosseoglou V (2010) Chickpea protein isolates obtained by wet extraction as emulsifying agents. J Sci Food Agric 90:304–313.  https://doi.org/10.1002/jsfa.3816 CrossRefPubMedGoogle Scholar
  56. Patel S, Cudney R, McPherson A (1994) Crystallographic characterization and molecular symmetry of edestin, a legumin from hemp. J Mol Biol 235:361–363.  https://doi.org/10.1016/S0022-2836(05)80040-3 CrossRefPubMedGoogle Scholar
  57. Pihlanto A (2006) Antioxidative peptides derived from milk proteins. Int Dairy J 16:1306–1314.  https://doi.org/10.1016/j.idairyj.2006.06.005 CrossRefGoogle Scholar
  58. Pihlanto A, Mattila P, Mäkinen S, Pajari AM (2017) Bioactivities of alternative protein sources and their potential health benefits. Food Funct 8:3443–3458.  https://doi.org/10.1039/C7FO00302A CrossRefPubMedGoogle Scholar
  59. Pojić M, Mišan A, Sakač M, Dapčević Hadnađev T, Šarić B, Milovanović I, Hadnađev M (2014) Characterization of byproducts originating from hemp oil processing. J Agric Food Chem 62:12436–12442.  https://doi.org/10.1021/jf5044426 CrossRefPubMedGoogle Scholar
  60. Pojić M, Dapčević Hadnađev T, Hadnađev M, Rakita S, Brlek T (2015) Bread supplementation with hemp seed cake: a by-product of hemp oil processing. J Food Qual 38(6):431–440.  https://doi.org/10.1111/jfq.12159 CrossRefGoogle Scholar
  61. Ponzoni E, Brambilla IM, Galasso I (2018) Genome-wide identification and organization of seed storage protein genes of Cannabis sativa. Biol Plant 62:693–702.  https://doi.org/10.1007/s10535-018-0810-7 CrossRefGoogle Scholar
  62. Pownall TL, Udenigwe CC, Aluko RE (2010) Amino acid composition and antioxidant properties of pea seed (Pisum sativum L.) enzymatic protein hydrolysate fractions. J Agric Food Chem 58:4712–4718.  https://doi.org/10.1021/jf904456r CrossRefPubMedGoogle Scholar
  63. Quiñones M, Sánchez D, Muguerza B, Moulay L, Laghi S, Miguel M, Aleixandre A (2010) Long-term intake of CocoanOX attenuates the development of hypertension in spontaneously hypertensive rats. Food Chem 122:1013–1019.  https://doi.org/10.1016/j.foodchem.2010.03.059 CrossRefGoogle Scholar
  64. Raikos V, Duthie G, Ranawana V (2015) Denaturation and oxidative stability of hemp seed (Cannabis sativa L.) protein isolate as affected by heat treatment. Plant Foods Hum Nutr 70:304–309.  https://doi.org/10.1007/s11130-015-0494-5 CrossRefPubMedGoogle Scholar
  65. Rajapakse N, Mendis E, Byun HG, Kim SK (2005) Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nutr Biochem 16:562–569.  https://doi.org/10.1016/j.jnutbio.2005.02.005 CrossRefPubMedGoogle Scholar
  66. Rezig L, Chibani F, Chouaibi M, Dalgalarrondo M, Hessini K, Guéguen J, Hamdi S (2013) Pumpkin (Cucurbita maxima) seed proteins: sequential extraction processing and fraction characterization. J Agric Food Chem 61:7715–7721.  https://doi.org/10.1021/jf402323u CrossRefPubMedGoogle Scholar
  67. Rodríguez-Ambriz SL, Martínez-Ayala AL, Millán F, Davila-Ortiz G (2005) Composition and functional properties of Lupinus campestris protein isolates. Plant Foods Hum Nutr 60:99–107.  https://doi.org/10.1007/s11130-005-6835-z CrossRefPubMedGoogle Scholar
  68. Rouimi S, Schorsch C, Valentini C, Vaslin S (2005) Foam stability and interfacial properties of milk protein–surfactant systems. Food Hydrocoll 19:467–478.  https://doi.org/10.1016/j.foodhyd.2004.10.032 CrossRefGoogle Scholar
  69. Ryan JT, Ross RP, Bolton D, Fitzgerald GF, Stanton C (2011) Bioactive peptides from muscle sources: meat and fish. Nutrients 3:765–791.  https://doi.org/10.3390/nu3090765 CrossRefPubMedPubMedCentralGoogle Scholar
  70. Singh U (1988) Antinutritional factors of chickpea and pigeonpea and their removal by processing. Plant Foods Hum Nutr 38:251–261.  https://doi.org/10.1007/BF01092864 CrossRefPubMedGoogle Scholar
  71. Singhal A, Karaca AC, Tyler R, Nickerson M (2016) Chapter 3: Pulse proteins: from processing to structure-function relationships. In: Goyal AK (ed) Grain legumes. IntechOpen, Rijeka, pp 55–78Google Scholar
  72. Söderberg J (2013) Functional properties of legume proteins compared to egg proteins and their potential as egg replacers in vegan food. Swedish University of Agricultural SciencesGoogle Scholar
  73. Tang CH, Ten Z, Wang XS, Yang XQ (2006) Physicochemical and functional properties of hemp (Cannabis sativa L.) protein isolate. J Agric Food Chem 54:8945–8950.  https://doi.org/10.1021/jf0619176 CrossRefPubMedGoogle Scholar
  74. Tang CH, Wang XS, Yang XQ (2009a) Enzymatic hydrolysis of hemp (Cannabis sativa L.) protein isolate by various proteases and antioxidant properties of the resulting hydrolysates. Food Chem 114:1484–1490.  https://doi.org/10.1016/j.foodchem.2008.11.049 CrossRefGoogle Scholar
  75. Tang CH, Peng J, Zhen DW, Chen Z (2009b) Physicochemical and antioxidant properties of buckwheat (Fagopyrumesculentum Moench) protein hydrolysates. Food Chem 115:672–678.  https://doi.org/10.1016/j.foodchem.2008.12.068 CrossRefGoogle Scholar
  76. Tcholakova S, Denkov ND, Ivanov IB, Campbell B (2006) Coalescence stability of emulsions containing globular milk proteins. Adv Colloid Interf Sci 123:259–293.  https://doi.org/10.1016/j.cis.2006.05.021 CrossRefGoogle Scholar
  77. Teh SS, Bekhit AE, Carne A, Birch J (2014) Effect of the defatting process, acid and alkali extraction on the physicochemical and functional properties of hemp, flax and canola seed cake protein isolates. J Food Meas Charact 8:92–104.  https://doi.org/10.1007/s11694-013-9168-x CrossRefGoogle Scholar
  78. Teh SS, Bekhit AE, Carne A, Birch J (2016) Antioxidant and ACE-inhibitory activities of hemp (Cannabis sativa L.) protein hydrolysates produced by the proteases AFP, HT, Pro-G, actinidin and zingibain. Food Chem 203:199–206.  https://doi.org/10.1016/j.foodchem.2016.02.057 CrossRefPubMedGoogle Scholar
  79. Vose JR (1980) Production and functionality of starches and protein isolates from legume seeds (field peas and horse beans). Cereal Chem 57:406–410Google Scholar
  80. Waggle DH, Steinke FH, Shen JL (1989) Isolated soy proteins. In: Matthews RH (ed) Legumes: chemistry, technology and human nutrition. Marcel Dekker, New York, pp 99–138Google Scholar
  81. Wang XS, Tang CH, Yang XQ, Gao WR (2008) Characterization, amino acid composition and in vitro digestibility of hemp (Cannabis sativa L.) proteins. Food Chem 107:11–18.  https://doi.org/10.1016/j.foodchem.2007.06.064 CrossRefGoogle Scholar
  82. Wang XS, Tang CH, Chen L, Yang XQ (2009) Characterization and antioxidant properties of hemp protein hydrolysates obtained with Neutrase®. Food Technol Biotechnol 47:428–434. ISSN:1330-9862Google Scholar
  83. Xu L, Diosady LL (2002) Removal of phenolic compounds in the production of high-quality canola protein isolates. Food Res Int 35:23–30.  https://doi.org/10.1016/S0963-9969(00)00159-9 CrossRefGoogle Scholar
  84. Yang HY, Yang SC, Chen JR, Tzeng YH, Han BC (2004) Soyabean protein hydrolysate prevents the development of hypertension in spontaneously hypertensive rats. Br J Nutr 92:507–512.  https://doi.org/10.1079/BJN20041218 CrossRefPubMedGoogle Scholar
  85. Yin SW, Tang CH, Wen QB, Yang XQ (2007) Properties of cast films from hemp (Cannabis sativa L.) and soy protein isolates. A comparative study. J Agric Food Chem 55:7399–7404.  https://doi.org/10.1021/jf071117a CrossRefPubMedGoogle Scholar
  86. Yin SW, Tang CH, Cao JS, Hu EK, Wen QB, Yang XQ (2008) Effects of limited enzymatic hydrolysis with trypsin on the functional properties of hemp (Cannabis sativa L.) protein isolate. Food Chem 106:1004–1013.  https://doi.org/10.1016/j.foodchem.2007.07.030 CrossRefGoogle Scholar
  87. Yin SW, Tang CH, Wen QB, Yang XQ (2009) Functional and structural properties and in vitro digestibility of acylated hemp (Cannabis sativa L.) protein isolates. Int J Food Sci Technol 44:2653–2661.  https://doi.org/10.1111/j.1365-2621.2009.02098.x CrossRefGoogle Scholar
  88. Zhang W, Yang YJ, Wang JJ (2008) Study on preparation of industrial hemp protein isolates [Chinese]. Sci Technol Food Ind 03. TS201.21Google Scholar
  89. Zhu KX, Zhou HM, Qian HF (2006) Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase. Process Biochem 41:1296–1302.  https://doi.org/10.1016/j.procbio.2005.12.029 CrossRefGoogle Scholar

Copyright information

© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Tamara Dapčević-Hadnađev
    • 1
    • 2
    Email author
  • Miroslav Hadnađev
    • 1
    • 2
  • Manda Dizdar
    • 2
  • Nataša Jovanović Lješković
    • 2
  1. 1.University of Novi Sad, Institute of Food TechnologyNovi SadSerbia
  2. 2.University Business Academy in Novi Sad, Faculty of PharmacyNovi SadSerbia

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