Influence of Citric Acid Concentration and Etching Time on Enamel Surface Roughness of Prepared Human Tooth: in vitro Study
In the present paper the average surface roughness Ra of three human enamel specimens subjected to etching using citric acid of different concentrations was evaluated using atomic force microscopy (AFM). The specimens were obtained from a single tooth. The cut sections of enamel were mounted in epoxy resin, ground and polished. Each specimen was etched using one of the concentrations (0.5 ωt. %, 3 ωt. %, 5 ωt. %) of citric acid. For each concentration five etching durations (1 s, 15 s, 30 s, 45 s, 60 s) were applied. The AFM images of similar size and resolution were evaluated for each sample after each etching duration. For each of the images Ra in three directions – horizontal, vertical and diagonal – was calculated and surface profiles constructed. The average value of Ra in three directions was taken as the absolute value of the roughness for a given surface. Dependence of the absolute value of the surface roughness upon the etching time for all samples was calculated.
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This work was supported by the grant of the Government of the Russian Federation (Grant No. 14.Z50.31.0046). E. V. Sadyrin was supported by the scholarship of the President of the Russian Federation no. SP-3672.2018.1. The tooth was extracted in the Dental Department of Rostov State Medical University Clinic. The research was approved by the ethical committee of Rostov State Medical University, the agreement from the patient was obtained. Experiments were conducted in Nanocenter of Research and Education Center “Materials” (http://nano.donstu.ru) and Chemistry Department of Don State Technical University.
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