Tripartite Interaction Among Nanoparticles, Symbiotic Microbes, and Plants: Current Scenario and Future Perspectives

  • Ovaid Akhtar
  • Ifra Zoomi
  • Dheeraj Pandey
  • Harbans Kaur Kehri
  • Raghvendra Pratap Narayan
Part of the Nanotechnology in the Life Sciences book series (NALIS)


In recent few decades, our understanding in the field of nanobiotechnology is increased dramatically. A number of nanoparticles (NPs) are synthesized by physical, chemical, as well as relatively safer green synthesis method. NPs seem to be the substitute for their respective bulk particles because of better action and sustained release. In agriculture production NPs could replace the use of chemical fertilizer in bulk, which otherwise deteriorates the soil health in the long term. However, because of unregulated use, it has posed negative impacts on ecosystems. NPs in the soil interact with the belowground microbes. The interaction reported till date is positive, negative, or neutral. For the application of engineered NPs in the agricultural field, it is of prime importance to understand the interaction among NPs, soil microbes, and plant roots. However, being a relatively new field, this understating is still at its beginning. This chapter reports about the interaction of NPs with symbiotic microbes, e.g., arbuscular mycorrhizal (AM) fungi, Rhizobia, etc. The mechanisms behind the reported interactions till date are generalized with the help of artwork. The chapter ends with the future perspectives of NPs along with the symbiotic soil microbes in increasing agronomy and soil heath.


Nanoparticles (NPs) AM fungi Nitrogen fixers P solubilizers Symbioses 



Arbuscular mycorrhiza


Carbon nanotubes


Dissolved organic carbon


Glomalin-related soil proteins


Multi-walled carbon nanotubes




Reactive oxygen species


  1. Arif N, Yadav V, Singh S, Tripathi DK, Dubey NK, Chauhan DK, Giorgetti L (2018) Interaction of copper oxide nanoparticles with plants: uptake, accumulation, and toxicity. In: Nanomaterials in plants, algae, and microorganisms. Academic Press, London, pp 297–310CrossRefGoogle Scholar
  2. Atha DH, Wang H, Petersen EJ et al (2012) Copper oxide nanoparticle mediated DNA damage in terrestrial plant models. Environ Sci Technol 46(3):1819–1827. Scholar
  3. Auffan M, Rose J, Bottero JY et al (2009) Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective. Nat Nanotechnol 4(10):634–641. Scholar
  4. Bandyopadhyay S, Peralta-Videa JR, Plascencia-Villa G et al (2012) Comparative toxicity assessment of CeO2 and ZnO nanoparticles towards Sinorhizobium meliloti, a symbiotic alfalfa associated bacterium: use of advanced microscopic and spectroscopic techniques. J Hazard Mater 241–242:379–386. Scholar
  5. Bandyopadhyay S, Plascencia-Villa G, Mukherjee A et al (2015) Comparative phytotoxicity of ZnO NPs, bulk ZnO, and ionic zinc onto the alfalfa plants symbiotically associated with Sinorhizobium meliloti in soil. Sci Total Environ 515–516:60–69. Scholar
  6. Cao J, Feng Y, Lin X et al (2016) Arbuscular mycorrhizal fungi alleviate the negative effects of iron oxide nanoparticles on bacterial community in rhizospheric soils. Front Environ 4:10. Scholar
  7. Cao J, Feng Y, Lin X et al (2017) Iron oxide magnetic nanoparticles deteriorate the mutual interaction between arbuscular mycorrhizal fungi and plant. J Soils Sediments 17(3):841–851. Scholar
  8. Doody M, Bais H, Jin Y (2014) Effects of citrate-coated silver nanoparticles on interactions between soil bacteria and the major crop plant Zea mays. In: EGU general assembly conference abstracts, Vienna, Austria, 27 April – 2 May, 2014Google Scholar
  9. Du W, Sun Y, Ji R et al (2011) TiO2 and ZnO nanoparticles negatively affect wheat growth and soil enzyme activities in agricultural soil. J Environ Monit 13(4):822–828. Scholar
  10. Feng Y, Cui X, He S et al (2013) The role of metal nanoparticles in influencing arbuscular mycorrhizal fungi effects on plant growth. Environ Sci Technol 47(16):9496–9504. Scholar
  11. Frenk S, Ben-Moshe T, Dror I et al (2013) Effect of metal oxide nanoparticles on microbial community structure and function in two different soil types. PLoS One 8(12):e84441. Scholar
  12. Ge Y, Schimel JP, Holden PA (2011) Evidence for negative effects of TiO2 and ZnO nanoparticles on soil bacterial communities. Environ Sci Technol 45(4):1659–1664. Scholar
  13. Ghasemi Siani N, Fallah S, Pokhrel LR et al (2017) Natural amelioration of Zinc oxide nanoparticle toxicity in fenugreek (Trigonella foenum-graecum) by arbuscular mycorrhizal (Glomus intraradices) secretion of glomalin. Plant Physiol Biochem 112:227–238. Scholar
  14. Hong J, Peralta-Videa JR et al (2014) Evidence of translocation and physiological impacts of foliar applied CeO2 nanoparticles on cucumber (Cucumis sativus) plants. Environ Sci Technol 48(8):4376–4385. Scholar
  15. Jing XX, Su ZZ, Xing HE et al (2016) Biological effects of ZnO nanoparticles as influenced by arbuscular mycorrhizal inoculation and phosphorus fertilization. Huan Jing Ke Xue 37(8):3208–3215. Scholar
  16. Kehri HK, Akhtar O, Zoomi I et al (2018) Arbuscular Mycorrhizal Fungi: Taxonomy and its Systematics. Int J Life Sci Res 6(4):58–71Google Scholar
  17. Kehri HK, Zoomi I, Singh U et al (2019) Review on biogenic synthesis of nanoparticles and their therapeutic applications: List of novel eco-friendly approaches. J Nanosci Tech 5(4):810–816Google Scholar
  18. Khodakovskaya M, Dervishi E, Mahmood M et al (2009) Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano 3(10):3221–3227. Scholar
  19. Koul A, Kumar A, Singh VK, Tripathi DK, Mallubhotla S (2018) Exploring plant-mediated copper, iron, titanium, and cerium oxide nanoparticles and their impacts. In: Nanomaterials in plants, algae, and microorganisms. Academic Press, London, pp 175–194CrossRefGoogle Scholar
  20. Kumari M, Khan SS, Pakrashi S et al (2011) Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa. J Hazard Mater 190(1–3):613–621. Scholar
  21. Larue C, Khodja H, Herlin-Boime N et al (2011) Investigation of titanium dioxide nanoparticles toxicity and uptake by plants. J Phys Conf Ser 304:012057. Scholar
  22. Lee CW, Mahendra S, Zodrow K et al (2010) Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana. Environ Toxicol Chem 29(3):669–675. Scholar
  23. Li S, Liu X, Wang F et al (2015) Effects of ZnO nanoparticles, ZnSO4 and arbuscular mycorrhizal fungus on the growth of maize. Huan Jing Ke Xue 36(12):4615–4622. Scholar
  24. McKee MS, Filser J (2016) Impacts of metal-based engineered nanomaterials on soil communities. Environ Sci Nano 3(3):506–533. Scholar
  25. Medina-Velo IA, Barrios AC, Zuverza-Mena N et al (2017) Comparison of the effects of commercial coated and uncoated ZnO nanomaterials and Zn compounds in kidney bean (Phaseolus vulgaris) plants. J Hazard Mater 332:214–222. Scholar
  26. Mishra S, Keswani C, Abhilash PC et al (2017) Integrated approach of agri-nanotechnology: challenges and future trends. Front Plant Sci 8:471. Scholar
  27. Mushtaq YK (2011) Effect of nanoscale Fe3O4, TiO2 and carbon particles on cucumber seed germination. J Environ Sci Health A 46(14):1732–1735. Scholar
  28. Pallavi, Mehta CM, Srivastava R et al (2016) Impact assessment of silver nanoparticles on plant growth and soil bacterial diversity. 3 Biotech 6(2):254. Scholar
  29. Pérez-de-Luque A (2017) Interaction of nanomaterials with plants: what do we need for real applications in agriculture? Front Environ Sci 5:12. Scholar
  30. Philippot L, Raaijmakers JM, Lemanceau P et al (2013) Going back to the roots: the microbial ecology of the rhizosphere. Nat Rev Microbiol 11(11):789–799. Scholar
  31. Rajput VD, Minkina T, Sushkova S et al (2018) Effect of nanoparticles on crops and soil microbial communities. J Soils Sediments 18(6):2179–2187. Scholar
  32. Raliya R, Franke C, Chavalmane S et al (2016) Quantitative understanding of nanoparticle uptake in watermelon plants. Front Plant Sci 7:1288. Scholar
  33. Rastogi A, Tripathi DK, Yadav S, Chauhan DK, Živčák M, Ghorbanpour M, El-Sheery NI, Brestic M (2019a) Application of silicon nanoparticles in agriculture. 3 Biotech 9(3):90CrossRefGoogle Scholar
  34. Rastogi A, Zivcak M, Tripathi DK, Yadav S, Kalaji HM, Brestic M (2019b) Phytotoxic effect of silver nanoparticles in Triticum aestivum: improper regulation of photosystem I activity as the reason for oxidative damage in the chloroplast. Photosynthetica 57(1):209–216CrossRefGoogle Scholar
  35. Sabo-Attwood T, Unrine JM, Stone JW et al (2012) Uptake, distribution and toxicity of gold nanoparticles in tobacco (Nicotiana xanthi) seedlings. Nanotoxicology 6(4):353–360. Scholar
  36. Sabourin V, Ayande A (2015) Commercial opportunities and market demand for nanotechnologies in agribusiness sector. J Technol Manag Innov 10(1):40–51. Scholar
  37. Shweta, Vishwakarma K, Sharma S, Narayan RP, Srivastava P, Khan AS, Dubey NK, Tripathi DK, Chauhan DK (2017) Plants and carbon nanotubes (CNTs) interface: present status and future prospects. In: Nanotechnology. Springer Singapore, Singapore, pp 317–340CrossRefGoogle Scholar
  38. Shweta, Tripathi DK, Chauhan DK, Peralta-Videa JR (2018) Availability and risk assessment of nanoparticles in living systems: a virtue or a peril? In: Nanomaterials in plants, algae, and microorganisms. Academic Press, London, pp 1–31Google Scholar
  39. Simonin M, Guyonnet JP, Martins JMF et al (2015) Influence of soil properties on the toxicity of TiO2 nanoparticles on carbon mineralization and bacterial abundance. J Hazard Mater 283:529–535. Scholar
  40. Simonin M, Richaume A, Guyonnet JP et al (2016) Titanium dioxide nanoparticles strongly impact soil microbial function by affecting archaeal nitrifiers. Sci Rep 6(1):33643. Scholar
  41. Singh S, Tripathi DK, Dubey NK, Chauhan DK (2016) Effects of nano-materials on seed germination and seedling growth: striking the slight balance between the concepts and controversies. Mater Focus 5(3):195–201CrossRefGoogle Scholar
  42. Singh A, Singh NB, Hussain I et al (2018) Plant-nanoparticle interaction: an approach to improve agricultural practices and plant productivity. Int J Pharm Sci Invent 4(8):25–40Google Scholar
  43. Singh J, Vishwakarma K, Ramawat N, Rai P, Singh VK, Mishra RK, Kumar V, Tripathi DK, Sharma S (2019) Nanomaterials and microbes’ interactions: a contemporary overview. 3 Biotech 9(3):68CrossRefGoogle Scholar
  44. Singhal U, Khanuja M, Prasad R et al (2017) Impact of synergistic association of ZnO-nanorods and symbiotic fungus Piriformospora indica DSM 11827 on Brassica oleracea var. botrytis (Broccoli). Front Microbiol 8:1909. Scholar
  45. Stampoulis D, Sinha SK, White JC (2009) Assay-dependent phytotoxicity of nanoparticles to plants. Environ Sci Technol 43(24):9473–9479. Scholar
  46. Su M, Liu H, Liu C et al (2009) Promotion of nano-anatase TiO2 on the spectral responses and photochemical activities of D1/D2/Cyt b559 complex of spinach. Spectrochim Acta A Mol Biomol Spectrosc 72(5):1112–1116. Scholar
  47. Suresh AK, Pelletier DA, Doktycz MJ (2013) Relating nanomaterial properties and microbial toxicity. Nanoscale 5(2):463–474. Scholar
  48. Taylor AF, Rylott EL, Anderson CWN et al (2014) Investigating the toxicity, uptake, nanoparticle formation and genetic response of plants to gold. PLoS One 9(4):e93793. Scholar
  49. Tripathi DK, Ahmad P, Sharma S, Chauhan DK, Dubey NK (eds) (2018) Nanomaterials in plants, algae, and microorganisms: concepts and controversies, vol 1. Academic Press, LondonGoogle Scholar
  50. Vishwakarma K, Upadhyay N, Kumar N, Tripathi DK, Chauhan DK, Sharma S, Sahi S (2018) Potential applications and avenues of nanotechnology in sustainable agriculture. In: Nanomaterials in plants, algae, and microorganisms. Academic Press, London, pp 473–500CrossRefGoogle Scholar
  51. Wang WZ, Wang FY, Li S et al (2014) Arbuscular mycorrhizal symbiosis influences the biological effects of nano-ZnO on maize. Huan Jing Ke Xue 35(8):3135–3141. Scholar
  52. Wang F, Liu X, Shi Z et al (2016) Arbuscular mycorrhizae alleviate negative effects of zinc oxide nanoparticle and zinc accumulation in maize plants – a soil microcosm experiment. Chemosphere 147:88–97. Scholar
  53. Wang F, Jing X, Adams CA et al (2018) Decreased ZnO nanoparticle phytotoxicity to maize by arbuscular mycorrhizal fungus and organic phosphorus. Environ Sci Pollut Res 25(24):23736–23747. Scholar
  54. Yang J, Cao W, Rui Y (2017) Interactions between nanoparticles and plants: phytotoxicity and defense mechanisms. J Plant Interact 12(1):158–169. Scholar
  55. Zoomi I, Narayan RP, Akhtar O et al (2017) Role of plant growth promoting rhizobacteria in reclamation of wasteland. In: Microbial biotechnology. Springer Singapore, Singapore, pp 61–80. Scholar

Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Ovaid Akhtar
    • 1
  • Ifra Zoomi
    • 2
  • Dheeraj Pandey
    • 2
  • Harbans Kaur Kehri
    • 2
  • Raghvendra Pratap Narayan
    • 3
  1. 1.Department of BotanyKamla Nehru Institute of Physical and Social SciencesSultanpurIndia
  2. 2.Sadasivan Mycopathology Laboratory, Department of BotanyUniversity of AllahabadAllahabadIndia
  3. 3.Netaji Subhash Chandra Bose Government Girls P.G. CollegeLucknowIndia

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