Nanotechnological Advances with PGPR Applications

  • A. R. Nayana
  • Bicky Jerin Joseph
  • Ashitha Jose
  • E. K. RadhakrishnanEmail author
Part of the Sustainable Agriculture Reviews book series (SARV, volume 41)


Plant growth promoting rhizobacteria (PGPR) are soil bacteria which have the potential for direct and indirect effects on plant growth. These organisms may have the capability to limit or replace the use of chemical fertilizers and inputs of toxic chemicals. Exploring PGPR in agriculture is, thus, one of the more promising techniques for increasing agricultural production without harming ecosystems. At the same time, nano-technological applications are greatly imparting their influence in agriculture. When compared against conventional fertilizers, nano-fertilizers play an effective role in promoting plant growth as they are rapidly absorbed by plants. Hence, nano-materials such as nano-fibers, nano-fertilizers and nano-pesticides may produce revolutionary effects in the agricultural sector. PGPR together with nanomaterials may thus be a favorable strategy for managing plant growth and productivity. The application of nanomaterials like silver, titanium, zinc oxide, silica, gold and others with PGPR holds great promise. However, there can be both positive and negative impacts of engineered metal nanoparticles on rhizobacteria. Hence, engineered nanoparticle (ENPs) must be studied further to explore their use as ecofriendly agents for field application. In this chapter we describe the effects of nanofertilizers with PGPR as an innovative method for improving crop productivity.


Agriculture Biofertilizer Nanofertilizer Nanoparticle PGPR 


  1. Aa E et al (2016) The silica-nano particles treatment of squash foliage and survival and development of Spodoptera littoralis (Bosid.) larvae. J Entomol Zool Stud 4(1):175–180Google Scholar
  2. Abdel Latef AAH et al (2018) Titanium dioxide nanoparticles improve growth and enhance tolerance of broad bean plants under saline soil conditions. Land Degrad Dev. John Wiley & Sons, Ltd, 29(4):1065–1073. CrossRefGoogle Scholar
  3. Aljabali AAA et al (2018) Synthesis of gold nanoparticles using leaf extract of Ziziphus zizyphus and their antimicrobial activity. Nanomaterials:1–15. PubMedCentralCrossRefGoogle Scholar
  4. Al-whaibi M, Mohammad F (2017) Role of nanoparticles in plants chapter 2 role of nanoparticles in plants. Nanotechnol Plant Sci (March). Google Scholar
  5. Arora S, Sharma P (2012) Gold-nanoparticle induced enhancement in growth and seed yield of Brassica juncea. Plant Growth Regul:303–310. CrossRefGoogle Scholar
  6. Ashkavand P et al (2015) Effect of SiO2 nanoparticles on drought resistance in hawthorn seedlings. De Gruyter 76(4):350–359. CrossRefGoogle Scholar
  7. Atici Ö, Aǧar G, Battal P (2005) Changes in phytohormone contents in chickpea seeds germinating under lead or zinc stress. Biol Plant 49(2):215–222. CrossRefGoogle Scholar
  8. Babu MY et al (2014) Application of biosynthesized silver nanoparticles in agricultural and marine application of biosynthesized silver nanoparticles in agricultural and marine Pest control. Curr Nanosci (May). CrossRefGoogle Scholar
  9. Bashan Y, de-Bashan LE, Prabhu SR, Hernandez JP (2014) Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013). Plant Soil 378(1–2):1–33CrossRefGoogle Scholar
  10. Basheer J (2017) Plant growth promoting potential of endophytic bacteria isolated from Piper nigrum plant growth promoting potential of endophytic bacteria isolated from Piper nigrum. Plant Growth Regul (September 2013). CrossRefGoogle Scholar
  11. Beneduzi A, Ambrosini A, Passaglia LMP (2012) Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents. Genet Mol Biol 4(35):1044–1051CrossRefGoogle Scholar
  12. Boonchuay P et al (2013) Effect of different foliar zinc application at different growth stages on seed zinc concentration and its impact on seedling vigor in rice. Soil Sci Plant Nutr 59(2):180–188. CrossRefGoogle Scholar
  13. Buzea C, Pacheco I (2017) Nanomaterials and their classification. In: EMR/ESR/EPR spectroscopy for characterization of nanomaterials. Springer, New Delhi, pp 3–45CrossRefGoogle Scholar
  14. Cakmak I, Kutman UB (2018) Agronomic biofortification of cereals with zinc: a review. Eur J Soil Sci 69(1):172–180. CrossRefGoogle Scholar
  15. Castañeda L et al (2014) Innovative Rice seed coating (Oryza Sativa) with polymer nanofibres and microparticles using the electrospinning method. J Res Updates Polym Sci 3(1):33–39. CrossRefGoogle Scholar
  16. Chaudhary V, Jangra S, Yadav NR (2018) Nanotechnology based approaches for detection and delivery of microRNA in healthcare and crop protection. J Nanobiotechnol 16(1):40CrossRefGoogle Scholar
  17. Dang H et al (2008) Dominant chloramphenicol-resistant bacteria and resistance genes in coastal marine waters of Jiaozhou Bay, China. World J Microbiol Biotechnol 24(2):209–217. CrossRefGoogle Scholar
  18. Dargo H, Ayaliew A, Kassa H (2017) Synthesis paradigm and applications of silver nanoparticles (AgNPs), a review. Sustain Mater Technol Elsevier 13(August):18–23. CrossRefGoogle Scholar
  19. De Gregorio PR, Michavila G, Muller LR, de Souza Borges C, Pomares MF, de Sa ELS, Pereira C, Vincent PA (2017) Beneficial rhizobacteria immobilized in nanofibers for potential application as soybean seed bioinoculants. PLoS One 12(5):e0176930PubMedPubMedCentralCrossRefGoogle Scholar
  20. Gao F et al (2008) Was improvement of spinach growth by nano-TiO(2) treatment related to the changes of Rubisco activase? Biometals. PubMedCrossRefPubMedCentralGoogle Scholar
  21. Gherasim O, Ficai A, Andronescu E (2018) Biomedical applications of silver nanoparticles : an up-to-date overview. Nanomaterials:1–25. PubMedCentralCrossRefGoogle Scholar
  22. Glick BR (2012) Plant growth-promoting bacteria: mechanisms and applications. Scientifica 2012Google Scholar
  23. Gordienko AS, Kurdish I (2007) Surface electrical properties of Bacillus subtilis cells and the effect of interaction with silicon dioxide particles. Biophysics. CrossRefGoogle Scholar
  24. Goudar KM et al (2018) Response of sunflower (Helianthus annuus L.) to nano boron nitride fertilization. Int J Chem Stud 6(5):2624–2630Google Scholar
  25. Hafeez B (2014) Role of zinc in plant nutrition- a review. Am J Exp Agric 3(2):374–391. CrossRefGoogle Scholar
  26. Haris Z, Ahmad I (2017) Impact of metal oxide nanoparticles on beneficial soil microorganisms and their secondary metabolites. Int J Life-Sci Sci Res 3(April):1020–1030. CrossRefGoogle Scholar
  27. Herizchi R et al (2014) Current methods for synthesis of gold nanoparticles. Artif Cells Nanomed Biotechnol. PubMedCrossRefPubMedCentralGoogle Scholar
  28. Jampílek J, Kráľová K (2017) Nanomaterials for delivery of nutrients and growth-promoting compounds to plants. In: Nanotechnology. Springer, Singapore, pp 177–226CrossRefGoogle Scholar
  29. Jasim B et al (2017) Plant growth and diosgenin enhancement effect of silver nanoparticles in Fenugreek (Trigonella foenum-graecum L.). Saudi Pharm J King Saud Univ 25(3):443–447. Scholar
  30. Kamran S et al (2017) Contribution of Zinc solubilizing bacteria in growth promotion and zinc content of Wheat. Front Microbiol 8(December).
  31. Karami A, Sepehri A (2018) Nano titanium dioxide and nitric oxide alleviate salt induced changes in seedling growth, physiological and photosynthesis attributes of barley. Zemdirbyste-Agric 105(2):123–132. CrossRefGoogle Scholar
  32. Karunakaran G et al (2013) Effect of nanosilica and silicon sources on plant growth promoting rhizobacteria, soil nutrients and maize seed germination. IET Nanobiotechnol (April):1–8. PubMedCrossRefGoogle Scholar
  33. Khan N, Bano A (2015) Role of plant growth promoting Rhizobacteria and Ag-nano particle in the bioremediation of heavy metals and maize growth under municipal wastewater irrigation. Int J Phytoremediation 6514(October). CrossRefGoogle Scholar
  34. Khati P et al (2017) Nanochitosan supports growth of Zea mays and also maintains soil health following growth. 3 Biotech. Springer Berlin Heidelberg 7(1):1–9.
  35. Khati P et al (2018) Effect of nanozeolite and plant growth promoting rhizobacteria on maize. 3 Biotech. Springer Berlin Heidelberg (February)
  36. Khoramdel RSSS (2016) Effects of Nano-zinc oxide and seed inoculation by plant growth promoting Rhizobacteria (PGPR) on yield, yield components and grain filling period of soybean (Glycine max L.). Iran J Field Crop Res 13(4):738–753Google Scholar
  37. Kumar V et al (2013) Science of the total environment gold nanoparticle exposure induces growth and yield enhancement in Arabidopsis thaliana. Sci Tot Environ The Elsevier B.V:461–462, 462–468. PubMedCrossRefPubMedCentralGoogle Scholar
  38. Larue C et al (2012) Comparative uptake and impact of TiO 2 nanoparticles in Wheat and Rapeseed. J Toxicol Environ Health Part A. CrossRefGoogle Scholar
  39. Lateef A et al (2016) Microporous and mesoporous materials synthesis and characterization of zeolite based nano e composite : an environment friendly slow release fertilizer. Microporous Mesoporous Mater. Elsevier Ltd 232:174–183. Scholar
  40. Laware SL, Raskar S (2014) Influence of Zinc oxide nanoparticles on growth, flowering and seed productivity in onion. Int J Appl Microbiol 3(7):874–881Google Scholar
  41. Lyu S et al (2017) Titanium as a beneficial element for crop production. Front Plant Sci 8(April):1–19. CrossRefGoogle Scholar
  42. Ma D et al (2017) Physiological responses and yield of wheat plants in Zinc-mediated alleviation of drought stress. Front Plant Sci 8(May):1–12. CrossRefGoogle Scholar
  43. Maiyappan S et al (2011) Isolation, evaluation and formulation of selected microbial consortia for sustainable agriculture. J Biofertil Biopestic 2(2):2–7. CrossRefGoogle Scholar
  44. Mahesh M et al (2018) Zeolite farming : a sustainable agricultural prospective. Int J Appl Sci 7(5):2912–2924Google Scholar
  45. Mala R et al (2017) Evaluation of Nano structured slow release fertilizer on the soil fertility, yield and nutritional profile of Vigna radiata. Recent Pat Nanotechnol 11(1):50–62. CrossRefPubMedPubMedCentralGoogle Scholar
  46. Malerba M, Cerana R (2016) Chitosan effects on plant systems. Int J Mol Sci 17(7):1–15. CrossRefGoogle Scholar
  47. Mao B et al (2018) Silver nanoparticles have lethal and sublethal adverse effects on development and longevity by inducing ROS-mediated stress responses. Sci Rep. Springer US (500):1–16.
  48. Maret W (2017) Zinc in cellular regulation : the nature and significance of “Zinc signals”. Int J Mol Sci. PubMedCentralCrossRefGoogle Scholar
  49. Maziah M, Zuraida AR, Halimi MS, Zulkifli HS, Sreeramanan S (2010) Influence of boron on the growth and biochemical changes in plant growth promoting rhizobacteria (PGPR) inoculated banana plantlets. World J Microbiol Biotechnol 26(5):933–944CrossRefGoogle Scholar
  50. Mccall KA, Huang C, Fierke CA (2000) Zinc and health : current status and future directions. Function and mechanism of zinc Metalloenzymes 1. Am Soc Nutr Sci 29(5):1437S–1446SGoogle Scholar
  51. Mendis HC, Thomas VP, Schwientek P, Salamzade R, Chien JT, Waidyarathne P, Kloepper J, De La Fuente L (2018) Strain-specific quantification of root colonization by plant growth promoting rhizobacteria Bacillus firmus I-1582 and Bacillus amyloliquefaciens QST713 in non-sterile soil and field conditions. PLoS One 13(2):e0193119PubMedPubMedCentralCrossRefGoogle Scholar
  52. Mirzaei M, Ali A, Safari A (2015) Aggregation stability and organic carbon fraction in a soil amended with some plant residues, nanozeolite, and natural zeolite. Int J Recycling Organic Waste Agric:11–22. CrossRefGoogle Scholar
  53. Mishra R, Sarma VV (2018) Secondary metabolite production by endophytic Fungi: the gene clusters, nature, and expression in. In: Jha S (ed) Endophytes and secondary metabolites. Springer, Dordrecht, pp 1–16. CrossRefGoogle Scholar
  54. Mohamed MA, Hashim AF, Alghuthaymi MA, Abd-Elsalam KA (2018) Nano-carbon: plant growth promotion and protection. In: Nanobiotechnology applications in plant protection. Springer, Cham, pp 155–188CrossRefGoogle Scholar
  55. Mousavi SR (2011) Zinc in crop production and interaction with phosphorus. Aust J Basic Appl Sci 5(9):1503–1509. Available at:
  56. Mukherjee A et al (2016) Carbon nanomaterials in agriculture: a critical review. Front Plant Sci 7(February):1–16. CrossRefGoogle Scholar
  57. Mumtaz MZ et al (2017) Zinc solubilizing Bacillus spp. potential candidates for biofortification in maize. Microbiol Res. Elsevier, 202(June):51–60. Scholar
  58. Obata H, Umebayashi M (1988) Effect of zinc deficiency on protein synthesis in cultured tobacco plant cells. Soil Sci Plant Nutr 34(3):351–357. CrossRefGoogle Scholar
  59. Palmqvist NGM et al (2015) Nano titania aided clustering and adhesion of beneficial bacteria to plant roots to enhance crop growth and stress management. Nature Publishing Group. Nature Publishing Group (December 2014):1–12.
  60. Pandey P, Bisht S, Sood A, Aeron A, Sharma GD, Maheshwari DK (2012) Consortium of plant-growth-promoting bacteria: future perspective in agriculture. In: Bacteria in agrobiology: plant probiotics. Springer, Berlin/Heidelberg, pp 185–200CrossRefGoogle Scholar
  61. Panichikkal J, Thomas R, John JC (2019) Biogenic gold nanoparticle supplementation to plant beneficial pseudomonas monteilii was found to enhance its plant probiotic effect. Curr Microbiol. Springer US 0(0):0. Scholar
  62. Park MR et al (2008) Influence of nanophase titania topography on bacterial attachment and metabolism. Int J Nanomedicine 3(4):497–504PubMedPubMedCentralGoogle Scholar
  63. Pestovsky YS, Martínez-antonio A (2017) The use of nanoparticles and Nanoformulations in agriculture. J Nanosci Nanotechnol 17(12):8699–8730. CrossRefGoogle Scholar
  64. Phenrat T et al (2009) Partial oxidation (“ Aging ”) and surface modification decrease the toxicity of Nanosized Zerovalent. Environ Sci Technol. CrossRefGoogle Scholar
  65. Prasad R, Kumar M, Kumar V (2017) Nanomaterials for delivery of nutrients and growth-promoting compounds to plants. Nanotechnol Agric Paradigm. Google Scholar
  66. Process DG (2013) Detonation gun process Zinc Biochemistry: from a single Zinc enzyme to a key element of life. Am Soc Nutr Adv Nutr (1):82–91.
  67. Qureshi A, Singh DK, Dwivedi S (2018) Nano-fertilizers: a novel way for enhancing nutrient use efficiency and crop productivity. Int J Curr Microbiol App Sci 7(2):3325–3335. CrossRefGoogle Scholar
  68. Radhakrishnan EK (2017) Microbially and phytofabricated AgNPs with different mode of bactericidal action were identified to have comparable potential for surface fabrication of central venous catheters to combat Staphylococcus aureus biofilm. J Photochem Photobiol B Biol Elsevier B.V. CrossRefGoogle Scholar
  69. Raghuvanshi P et al (2017) Biosynthesis and characterization of gold nanoparticles from plant growth promoting rhizobacteria. Int J Chem Stud 5(5):525–532Google Scholar
  70. Ramesh K, Reddy DD (2011) Zeolites and their potential uses in agriculture. Adv Agron 113. Google Scholar
  71. Ramprasad D, Sahoo D, Sreedhar B (2015) Plant growth promoting Rhizobacteria – an overview. Eur J Biotechnol Biosci 2(2):30–34Google Scholar
  72. Rangaraj S et al (2014) Effect of silica nanoparticles on microbial biomass and silica availability in maize rhizosphere. Biotechnol Appl Biochem. PubMedCrossRefPubMedCentralGoogle Scholar
  73. Rashid R, Murtaza G, Zahra A (2014) Gold nanoparticles: synthesis and applications in drug. Trop J Pharm Res 13(July):1169–1177Google Scholar
  74. Rastogi A et al (2019) Application of silicon nanoparticles in agriculture. 3 Biotech. Springer International Publishing 9(3):1–11.
  75. Roshmi T et al (2015) Effect of biofabricated gold nanoparticle-based antibiotic conjugates on minimum inhibitory concentration of bacterial isolates of clinical origin. Gold Bull:63–71. CrossRefGoogle Scholar
  76. Sangeetha C, Baskar P (2016) Zeolite and its potential uses in agriculture : a critical review. Agric Rev 37(2):101–108. CrossRefGoogle Scholar
  77. Savithramma N, Ankanna S, Bhumi G (2012) Effect of nanoparticles on seed germination and seedling growth of Boswellia ovalifoliolata an endemic and endangered medicinal tree taxon. Nano Vision 2(1):2Google Scholar
  78. Shah M et al (2014) Gold nanoparticles: various methods of synthesis and antibacterial applications. Fron Biosci (Landmark Ed) 1(1):1320–1344CrossRefGoogle Scholar
  79. Shalaby TA, Bayoumi Y, Abdalla N, Taha H, Alshaal T, Shehata S, Amer M, Domokos-Szabolcsy É, El-Ramady H (2016) Nanoparticles, soils, plants and sustainable agriculture. In: Nanoscience in food and agriculture 1. Springer, Cham, pp 283–312CrossRefGoogle Scholar
  80. Sharma A, Patni B, Shankhdhar D (2013) Zinc – an indispensable micronutrient. Physiol Mol Biol Plants 19(March):11–20. CrossRefPubMedPubMedCentralGoogle Scholar
  81. Sharma D, Kanchi S, Bisetty K (2015) Biogenic synthesis of nanoparticles : a review. Arab J Chem. CrossRefGoogle Scholar
  82. Shireen F et al (2018) Boron : functions and approaches to enhance its availability in plants for sustainable agriculture. Int J Mol Sci:95–98. PubMedCentralCrossRefPubMedGoogle Scholar
  83. Shukla SK et al (2015) Prediction and validation of gold nanoparticles (GNPs) on plant growth promoting rhizobacteria (PGPR): a step toward development of. De Gruyter 4(5):439–448. CrossRefGoogle Scholar
  84. Siddiqui MH, Al-whaibi MH (2014) Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds mill.). Saudi J Biol Sci. King Saud Univ 21(1):13–17. Scholar
  85. Siddiqui MH, Al-Whaibi MH, Mohammad F (2015) Nanotechnology and plant sciences: nanoparticles and their impact on plants. Nanotechnol Plant Sci Nanopart Impact Plants:1–303. Google Scholar
  86. Stierle AA, Stierle DB (2016) Bioactive secondary metabolites produced by the fungal endophytes of conifers. HHS Public Access 10(10):1671–1682Google Scholar
  87. Strout G et al (2013) Silica nanoparticles aid in structural leaf coloration in the Malaysian tropical rainforest understorey herb Mapania caudata. Ann Bot:1141–1148. PubMedPubMedCentralCrossRefGoogle Scholar
  88. Sultana U, Desai S, Reddy G, TNKVV P (2019) Zinc solubilizing plant growth promoting microbes produce zinc nanoparticles. bioRxiv:602219Google Scholar
  89. Suriyaprabha R et al (2012) Growth and physiological responses of maize (Zea mays L.) to porous silica nanoparticles in soil. J Nanopart Res 14(12).
  90. Timmusk S et al (2017) Perspectives and challenges of microbial application for crop improvement. Front Plant Sci 8(February):1–10. CrossRefGoogle Scholar
  91. Timmusk S, Seisenbaeva G, Behers L (2018) Titania (TiO2) nanoparticles enhance the performance of growth-promoting rhizobacteria. Sci Rep. Springer US:1–13.
  92. Tong Z, Bischoff M, Nies L, Applegate B, Turco RF (2007) Impact of fullerene (C60) on a soil microbial community. Environ Sci Technol 41(8):2985–2991PubMedCrossRefPubMedCentralGoogle Scholar
  93. Wang H, Liu RL, Jin JY (2009) Effects of zinc and soil moisture on photosynthetic rate and chlorophyll fluorescence parameters of maize. Biol Plant 53(1):191–194. CrossRefGoogle Scholar
  94. Wang XP et al (2018) Effects of zinc oxide nanoparticles on the growth, photosynthetic traits, and antioxidative enzymes in tomato plants. Biol Plant 62(4):801–808. CrossRefGoogle Scholar
  95. Yılmaz E, Sönmez İ, Demir H (2014) Effects of zeolite on seedling quality and nutrient contents of cucumber plant (Cucumis sativus L. cv. Mostar F1) grown in different mixtures of growing media. Commun Soil Sci Plant Anal 45(21):2767–2777CrossRefGoogle Scholar
  96. Yin L, Colman BP, McGill BM, Wright JP, Bernhardt ES (2012) Effects of silver nanoparticle exposure on germination and early growth of eleven wetland plants. PLoS One 7(10):e47674. CrossRefPubMedPubMedCentralGoogle Scholar
  97. Yu G (2018) Root exudates and microbial communities drive mineral dissolution and the formation of Nano-size minerals in soils: implications for soil carbon storage. Root Biol 52(May). Google Scholar

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Authors and Affiliations

  • A. R. Nayana
    • 1
  • Bicky Jerin Joseph
    • 1
  • Ashitha Jose
    • 1
  • E. K. Radhakrishnan
    • 1
    Email author
  1. 1.School of BiosciencesMahatma Gandhi UniversityKottayamIndia

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