The Optical Clearing Method pp 1-15 | Cite as
Tissue Optics
Abstract
Light interaction with biological materials depends on the material’s optical properties. From those properties, the absorption and scattering coefficients are the most important, since they quantify how much of a light beam is attenuated when traveling inside a tissue. The scattering coefficient is known to be significantly higher than the absorption coefficient in biological materials, meaning that most of the light is scattered, turning optical methods in clinical practice limited. Such difference between the scattering and absorption coefficients is mainly due to a refractive index mismatch between tissue components and fluids. We explain this concept in the present chapter before introducing the technique that efficiently minimizes this effect in the following chapters.
Keywords
Absorption coefficient Scattering coefficient Anisotropy factor Reduced scattering coefficient Light attenuation Light penetration depth Refractive index mismatchReferences
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