Enzyme Discovery and Selection

  • Pablo Carbonell
Part of the Learning Materials in Biosciences book series (LMB)


Enzymes have the ability of catalyzing reactions. Each organism has evolved its very own version of an enzyme. Having to select an enzyme sequence for a target reaction is not always straightforward. In some cases, we may have hundreds of variants coming from different organisms to choose from and with little information about the best choice. In other cases, no known enzyme sequence catalyzing the desired reaction would be known. In this chapter, you will learn some basic techniques based on homology modeling both of the sequence and the chemical reaction allowing to guide us when selecting enzyme sequence candidates. Based on such approaches, at the end of this chapter we will discuss how to expand natural capabilities of enzymes through promiscuity.


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Further Reading

  1. A good introduction to sequence similarity searching:Google Scholar
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  3. A good introduction to protein structure analysis using PyMOL:Google Scholar
  4. Skern, T.: Exploring Protein Structure: Principles and Practice. Learning Materials in Biosciences. Springer International Publishing, Cham (2018)CrossRefGoogle Scholar
  5. EC-BLAST provides a good example of a tool for enzyme discovery and selection focused on the reaction:Google Scholar
  6. Rahman, S.A., Cuesta, S.M., Furnham, N., Holliday, G.L., Thornton, J.M.: EC-BLAST: a tool to automatically search and compare enzyme reactions. Nat. Methods 11(2), 171–174 (2014)CrossRefGoogle Scholar
  7. A formal introduction to extended metabolic space modeling:Google Scholar
  8. Carbonell, P., Delépine, B., Faulon, J.L.: Extended metabolic space modeling. In: Methods in Molecular Biology, vol. 1671, pp. 83–96 (2018)Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Pablo Carbonell
    • 1
  1. 1.Manchester Institute of BiotechnologyUniversity of ManchesterManchesterUK

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