Protocols for Cellular Evaluation of Targeted Drug Delivery Systems for Cancer and Infectious Diseases

  • Aakruti Kaikini
  • Vaibhavi Peshattiwar
  • Padma V. Devarajan
  • Prajakta DandekarEmail author
  • Sadhana SathayeEmail author
Part of the AAPS Advances in the Pharmaceutical Sciences Series book series (AAPS, volume 39)


The most critical stage in evaluation of a novel drug or its delivery system is assessment of its safety and efficacy. Traditionally, in vivo animal models were used for this assessment. However, due to growing ethical concerns in animal usage, these in vivo animal models have largely been replaced by cell-based assays. Cell-based assays offer several advantages which have been described in this section. This chapter describes in detail the protocols along with critical parameters for various cell-based assays which can be used for evaluation of targeted drug delivery systems for cancer and infectious diseases.


Cell-based assays In vitro Cancer Infectious diseases 



3H-labeled thymidine


American Type Culture Collection


Basic fibroblast growth factor




Chick embryo chorioallantoic membrane


Colony-forming units


The Clinical and Laboratory Standards Institute


Dimethyl sulfoxide




Endothelial basal media


Ethylenediaminetetraacetic acid


(Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid)


European Society of Clinical Microbiology and Infectious Diseases


Fluorescein isothiocyanate


Forster resonance energy transfer


High performance liquid chromatography


Human umbilical vein endothelial cells


Liquid chromatography-mass spectrometry


Mueller–Hinton broth


Minimum inhibitory concentration


(3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)


Novel drug delivery system


Post antibiotic effect


Propidium iodide




Vascular endothelial growth factor


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Copyright information

© American Association of Pharmaceutical Scientists 2019

Authors and Affiliations

  • Aakruti Kaikini
    • 1
  • Vaibhavi Peshattiwar
    • 1
  • Padma V. Devarajan
    • 2
  • Prajakta Dandekar
    • 2
    Email author
  • Sadhana Sathaye
    • 1
    Email author
  1. 1.Department of Pharmaceutical Sciences & TechnologyInstitute of Chemical TechnologyMumbaiIndia
  2. 2.Department of Pharmaceutical SciencesInsitute of Chemical Technology, Deemed University, Elite Status and Centre of Excellence, Government of MaharashtraMumbaiIndia

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