Protocols for Cellular Evaluation of Targeted Drug Delivery Systems for Cancer and Infectious Diseases
The most critical stage in evaluation of a novel drug or its delivery system is assessment of its safety and efficacy. Traditionally, in vivo animal models were used for this assessment. However, due to growing ethical concerns in animal usage, these in vivo animal models have largely been replaced by cell-based assays. Cell-based assays offer several advantages which have been described in this section. This chapter describes in detail the protocols along with critical parameters for various cell-based assays which can be used for evaluation of targeted drug delivery systems for cancer and infectious diseases.
KeywordsCell-based assays In vitro Cancer Infectious diseases
American Type Culture Collection
Basic fibroblast growth factor
Chick embryo chorioallantoic membrane
The Clinical and Laboratory Standards Institute
Endothelial basal media
(Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid)
European Society of Clinical Microbiology and Infectious Diseases
Forster resonance energy transfer
High performance liquid chromatography
Human umbilical vein endothelial cells
Liquid chromatography-mass spectrometry
Minimum inhibitory concentration
(3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)
Novel drug delivery system
Post antibiotic effect
Vascular endothelial growth factor
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