Bioscience Engineering (Biological Engineering)
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This chapter (Bioscience Engineering) begins with a description of the emerging field of bioscience engineering (biological engineering). The chapter continues with a description of the relationship of amino acid sequence and the three-dimensional structure of proteins. This topic will be the central concept for the students’ introduction to bioscience engineering (bioengineering) and its importance will become evident as the reader proceeds in reading the book. The chapter describes the earliest technique used for protein folding, X-ray diffraction crystallography. X-ray diffraction crystallography was the first technique used to visualize and eventually classify proteins. The genetic code and alternative splicing are reviewed. Collections of proteins and their structures are described in this chapter. They include The Human Genome Project, The Protein Data Bank, and the Human Proteome Project. The earliest theory for how proteins fold, Anfinsen’s thermodynamic hypothesis, and its dependence on Gibbs free energy are presented.
Occurs when splicing of pre-mRNA to form mRNA results in more than one protein due to differential splicing.
Is the code used by which the mRNA sequence determines the amino acid sequence of a protein at the ribosome.
Is the driving force of a reaction. A spontaneous reaction occurs if the free energy is negative and does not occur if it is positive.
Has sequenced the entire euchromatic DNA of humans.
Maps the entire number of human proteins.
Involves the reaction of the protein with heavy elements. Crystallography results in a protein heavy metal crystal which supplies X-ray diffraction pictures which are easier to analyze.
Uses a protein of known three-dimensional structure to solve the conformation of a related protein.
Are proteins that conduct their function. If exposed to certain chemicals or heat, the proteins become nonfunctional and thereby are denatured. Removal of the denaturation agent restores them to their proper function (renaturation). Denaturation represents unfolding of the protein. If the protein once denatured cannot be restored to its normal function, it is said to be coagulated.
Is a worldwide depository of proteins.
Are protein regions that bind molecules. The type of recognition site determines the classification of the protein as immunoglobulin, enzyme, structural, transport, or hormone.
Is the degree of “fuzziness” of the X-ray diffraction pattern. In modern times most X-ray diffraction patterns use 2 Å of resolution.
Uses X-rays to strike a protein thus producing diffracted secondary waves which are used to ascertain the three-dimensional structure of the protein usually with the help of the amino acid sequence.
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