Detection and Identification Technologies for CBRN Agents

  • Olivier MattmannEmail author


This chapter provides an overview of available technologies for the detection and identification of agents released (alone or in mixtures), at the scene of a major intentional, accidental or natural CBRNE incident. These technologies are crucial to ensure adequate risk assessment, optimal risk management, and proper counter-measures including medical treatment, personal protection and restored access to infrastructure. In addition, monitoring/screening of these agents (and their precursors/dual agents) is also essential during production, storage and transport.


  1. Abingdonhealth. n.d. What is a lateral flow immunoassay?
  2. Adamovica, M. 2017. Gas detection: How catalytic bead sensors work and why O2 sensors are omportant. PK Safety. Accessed 8 Apr 2018.
  3. Agilent Technologies. 2011. Webinar, microwave plasma – Atomic emission spectroscopy, a revolutionary new technique and plasma source that increases performance while eliminating expensive gas requirements., Accessed 8 Apr 2019.
  4. AJP- 3.15. 2018. Allied Joint Doctrine for countering improvised explosive devices. Edition C Version 1, February 2018. Available at
  5. Anderson, G. P, and C.A. Rowe-Taitt. 2000. Water quality monitoring using an automated portable fiber optic biosensor: RAPTOR. Photonic detection and intervention technologies for safe food. Proc. SPIE 2000, 4206, 418742.Google Scholar
  6. Antec Scientific. n.d. Electrochemical detection. Accessed 8 Apr 2019.
  7. Bakour, S., J. Rathored, S. Sankar, P. Biagini. 2016. Identification of virulence factors and antibiotic resistance markers using bacterial genomics. Future Microbiology, March 2016. DOI: Scholar
  8. Beck, K. 2018. The types of electrophoresis. Sciencing (25 July 2018).
  9. Bergström, T. 2016. Protein identification and characterization through peptide mass spectrometry. Method development for improved ricin and botulinum neurotoxin analysis. Doctoral Thesis, Swedish University of Agricultural Sciences, Umeå.Google Scholar
  10. Carey, J.R., K.S. Suslick, K.I. Hulkower, et al. 2011. Rapid identification of bacteria with a disposable colorimetric sensing array. Journal of the American Chemical Society 133: 7571–7576.CrossRefGoogle Scholar
  11. CDC. 2012. Possession, use, and transfer of select agents and toxins, centers for disease control and prevention, department of health and human services. Biennial Review; Final Rule Federal Register 77 (194): 61083–61115. Scholar
  12. CERN. n.d. Solid state detectors. Available at
  13. Chalmers, J. M. 2013. Infrared spectroscopy, reference module in chemistry, molecular sciences and chemical engineering. Elsevier pp. 402–415. Available at Accessed 8 Apr 2019.
  14. Chasteen, T.G. 2009a. Flame photometric GC detector. The Chemiluminescence Home Page. Accessed 7 Apr 2019.
  15. ———. 2009b. The flame ionization detector. Sam Houston State University.
  16. Clarke, W. 2017. Chapter 1 – Mass spectrometry in the clinical laboratory: Determining the need and avoiding pitfalls. In Mass spectrometry for the clinical laboratory, vol. 2017. Elsevier. Scholar
  17. Davies, A.M.C. n.d. An introduction to near infrared (NIR) spectroscopy, IMPublications website. Accessed 8 Apr 2019.
  18. Dembek, Z.F., ed. 2011. USAMRIID’s medical management of biological casualties handbook. 7th ed. Fort Detrick: U.S. Army Medical Research Institute of Infectious Diseases.Google Scholar
  19. Dutrow, B.L, and C.M. Clark. n.d. X-ray powder diffraction (XRD). The Science Education Research Center at Carleton College. Accessed 8 Apr 2019.
  20. El Sohly, M.A., W. Gul, and M. Salem. 2008. Chapter 5 – Cannabinoids analysis: analytical methods for different biological specimens. In Handbook of analytical separations, vol. 6, 203–241. Elsevier. Accessed 7 Apr 2019.Google Scholar
  21. European Nuclear Society (ENS). n.d. Monitoring area. Accessed 23 Mar 2019.
  22. FAIMS website. n.d. What is high-field asymmetric waveform ion mobility spectrometry?
  23. Favrot, C. 2015. Polymerase chain reaction: Advantages and drawbacks. In 3. Congresso Latinoamericano de Dermatologia Veterinaria, 26–27. Argentina: Buenos Aires.Google Scholar
  24. Feltis, B.N., B.A. Sexton, F.L. Glenn, et al. 2008. A Hand-held surface plasmon resonance biosensor for the detection of ricin and other biological agents. Biosensors and Bioelectronics 23: 1131–1136.CrossRefGoogle Scholar
  25. Feng, S., R. Caire, B. Cortazar, et al. 2014. Immunochromatographic diagnotic test analysis using google glass. ACS Nano 8: 3069–3079.CrossRefGoogle Scholar
  26. Fornara, A., P. Johansson, K. Petersson, et al. 2008. Tailored magnetic nanoparticles for direct and sensitive detection of biomolecules in biological samples. Nano Letters 2008 (8): 3423–3428.CrossRefGoogle Scholar
  27. Gannon, M. 2018. An unknown ‘disease X’ could become an epidemic. Can we find it before it’s too late? LifeScience (18 Oct. 2018).
  28. Ghann, W., and J. Uddin. 2017. Terahertz (THz) spectroscopy: a cutting-edge technology. DOI 10.5772/67031. Accessed 8 Apr 2019.CrossRefGoogle Scholar
  29. Hao, R.Z., H.B. Song, G.M. Zuo, et al. 2011. Probe functionalized QCM biosensor based on gold nanoparticle amplification for bacillus anthracis detection. Biosensors and Bioelectronics (26): 3398–3404.CrossRefGoogle Scholar
  30. Hitoshi, K., F. Yosuke, D.M. Joel, et al. 2018. Vapor detection and discrimination with a panel of odorant receptors. Nature Communications 9 (1).
  31. Ho, Y.P., and P.M. Reddy. 2010. Identification of pathogens by mass spectrometry. Clinical Chemistry 56: 525–536.CrossRefGoogle Scholar
  32. Jamshaid, T., E.T.T. Neto, M.M. Eissa, et al. 2016. Magnetic particles: From preparation to lab-on-a-chip, biosensors, microsystems and microfluidics applications. Trends in Analytical Chemistry 79: 344–362.CrossRefGoogle Scholar
  33. JoVE. 2019. Gas chromatography (GC) with flame-ionization detection. Science Education Database. Analytical Chemistry. Cambridge. Accessed 7 Apr 2019.
  34. Krammer, M. n.d. Scintillators. Vienna: Institute of High Energy Physics. Available at
  35. KROMEK website. n.d. About CZT: cadmium zinc telluride. Available at
  36. Kwak, S.-W., S.-S. Chang, and H.-S. Yoo. 2010. Radiation detection system for prevention of illicit trafficking of nuclear and radioactive materials. Journal of Radiological Protection 35 (4): 167–171. Scholar
  37. Lamont-Doherty Earth Observatory. n.d. Ion chromatograph to detect major anions in precipitation (snow), groundwaters and drinking waters from New York. Earth Institute, Columbia University. Accessed 7 Apr 2019.
  38. LCGC. n.d. Quadrupole mass analyzers: An introduction. LCGC Europe 25(11).Google Scholar
  39. Li, D., Y. Feng, L. Zhou, et al. 2011. Label-Free capacitive immunosensor based on quartz crystal Au electrode for rapid and sensitive detection of Escherichia coli O157: H7. Analytica Chimica Acta 687: 89–96.CrossRefGoogle Scholar
  40. Long, M. Scott. 2019. Basic principles of LC, HPLC, MS, & MS. Chemix website. Accessed 8 Apr 2019.
  41. Lonsdale, C.L., B. Taba, N. Queralto, et al. 2013. The use of colorimetric sensor arrays to discriminate between pathogenic bacteria. PLoS One 2013 (8): e62726.CrossRefGoogle Scholar
  42. MacDonald, J., et al. 2003. Alternatives for landmine detection. RAND.Google Scholar
  43. Mariani, S., and M. Minunni. 2014. Surface plasmon resonance applications in clinical analysis. Analytical and Bioanalytical Chemistry 406: 2303–2323.CrossRefGoogle Scholar
  44. Maziejuk, M., W. Lisowski, M. Szyposzynska, T. Sikora, and A. Zalewska. 2015. Differential ion mobility spectrometry in application to the analysis of gases and vapors. Solid State Phenomena 223.CrossRefGoogle Scholar
  45. Mcdermott, J. 2016. Professor designs explosives detector to rival a dog’s nose. (16 February 2016).
  46. Med.Navy.Mil. n.d. Information paper: Hand held assay (HHA).
  47. MedPhys. n.d. Chapter 4: Scintillation detectors. In Radioisotopes and radiation methodology. Med Phys 4R06/6R03, pp. 4-1 to 10.
  48. Meenakshi, A. 2018. Cell culture media: A review. Mater Methods 3:175. (Revised 11 Nov 2018).
  49. Mellon, F.A. 2003. Mass spectrometry – Principles and instrumentation. In Encyclopedia of food sciences and nutrition, 2nd ed. Elsevier.Google Scholar
  50. Miglierini, M. 2004. Detectors of radiation. E. Wigner Course on Reactor Physics Experiments, April 27 – May 15, 2004, at
  51. Miles, S. 1997. Rapid purification of small molecule libraries by ion exchange chromatography. Tetrahedron Letters. 38:3357–3358. Via Elsevier Science Direct.Google Scholar
  52. MSA. n.d. Photo ionization detectors (PIDs). Theory, uses and applications, MSA.
  53. Nanophoton. n.d. What is Raman spectroscopy? Accessed 8 Apr 2019.
  54. Nationalmaglab. n.d.-a. Tandem mass spectrometry (MS/MS). National High Magnetic Field Laboratory.
  55. ———. n.d.-b. Matrix-assisted laser desorption ionization (MALDI). National High Magnetic Field Laboratory at
  56. NCRJS. n.d. Immunoassay technologies, pp. 29–30. At
  57. Neubauer, K. 2009. Advantages and disadvantages of different column types for speciation analysis by LC-ICP-MS. Spectroscopy Online. Accessed 8 Apr 2019.
  58. Ong, T.-H., T. Mendum, G. Geurtsen, et al. 2017. Use of mass spectrometric vapor analysis to improve canine explosive detection efficiency. Analytical Chemistry 89 (12): 6482–6490.CrossRefGoogle Scholar
  59. Priest, J.R. 2017. A primer to clinical genome sequencing. Current Opinion in Pediatrics (29): 513–519.CrossRefGoogle Scholar
  60. Prime Faraday Technology Watch. 2002. An introduction to MEMS (Micro-electromechanical Systems). Accessed 8 Apr 2019.
  61. Radboud University, Faculty of Science. n.d. About NMR (nuclear magnetic resonance). Accessed 8 Apr 2019.
  62. Rebmann, A., M.H. Sorg, and E. David. 2000. Cadaver dog handbook. Boca Raton, London, New York, Washington D.C.: CRC Press LLC.Google Scholar
  63. Reusch, W. 2013. Visible and ultraviolet spectroscopy. Michigan State University, Department of Chemistry. Accessed 8 Apr 2019.
  64. Ridha, A.-A. n.d. Chapter 7 – Nuclear detectors. Nuclear Physics 80–92.
  65. Sanchez-Rodas, D., W.T. Corns, B. Chen, and P.B. Stockwell. 2010. Atomic fluorescence spectrometry: A suitable detection technique in speciation studies for arsenic, selenium, antimony and mercury. Journal of Analytical Atomic Spectrometry 25: 933–946.CrossRefGoogle Scholar
  66. SANDIA. 2011. Rapid, automated point-of-care system (RapiDx). Accessed 7 Apr 2019.
  67. ———. 2013. SpinDx™: Point-of-care diagnostics using centrifugal microfluidics. Accessed 7 Apr 2019.
  68. SAVER. 2005. Guide for the selection of chemical agent and toxic industrial material detection equipment for emergency first responders, Guide 100-04, Volume I and II: Summary; pp. 1–5.Google Scholar
  69. Schelkanova, I., A. Pandya, A. Muhaseen, G. Saiko, and A. Douplik. 2015. Chapter 13 – Early optical diagnosis of pressure ulcers. In Biophotonics for medical applications, vol. 2015, 347–375. Woodhead Publication Series Biomaterials Elsevier.Google Scholar
  70. Semrock website. n.d. Surface-enhanced Raman scattering (SERS). Accessed 8 Apr 2019.
  71. Senseor. n.d. How SAW sensors operate?
  72. Seo, Y., J.-e Kim, Y. Jeong, et al. 2016. Engineered nanoconstructs for the multiplexed and sensitive detection of high-risk pathogens. Nanoscale 8: 1944–1951.CrossRefGoogle Scholar
  73. Sevostianova, E. n.d. Atomic absorption spectroscopy. Accessed 8 Apr 2019.
  74. Sferopoulos, R. 2008. A review of chemical warfare agent (CWA) detector technologies and commercial-off-the-shelf items. Human Protection and Performance Division, Defense Science and Technology Organization, Australian Government. DSTO-GD-0570.Google Scholar
  75. Siebach, J. 2010. Characterization of He-3 detectors typically used in international safeguards monitoring, Thesis, Brigham Young University.
  76. Stauffer, E., J.A. Dolan, and R. Newman. 2008. Chapter 5 – Detection of ignitable liquid residues at fire scenes. In Fire debris analysis, vol. 2008, 131–161. Elsevier. Accesses 7 Apr 2019.CrossRefGoogle Scholar
  77. Sun, Y., and K.Y. Ong. 2005. Detection technologies for chemical warfare agents and Toxic Vapors. 1st ed, 272. Boca Raton: CRC Press.Google Scholar
  78. Taitt, C.R., L.C. Shriver-Lake, M.M. Ngundi, and F.S. Ligler. 2008. Array biosensor for toxin detection: Continued advances. Sensors 8: 8361–8377.CrossRefGoogle Scholar
  79. Terelii, M., and A. Tüzüni. 2014. New molecular methods for detection of bioterrorism agents. Türk Bilimsel Derlemeler Dergisi 7 (1): 46–48.Google Scholar
  80. Thakur, S.N. 2007. Chapter 2 – Atomic emission spectroscopy. In Laser-induced breakdown spectroscopy, 23–48. Elsevier.Google Scholar
  81. The Sam Houston State University. n.d. The pulsed flame photometric detector.
  82. Thomas III, S.W., Joly, G.D. and T.M. Swager. 2007. Chemical sensors based on amplifying fluorescent conjugated polymers, Chemistry Review vol. 107, 1339−1386. Accessed 8 Apr 2019.
  83. Tomšič, U. 2003. Detection of explosives: Dogs vs. CMOS capacitive sensors. University of Ljubljana, Faculty of Mathematics and Physics, Department of Physics. Seminars (March 2003). Available at
  84. University of Massachusetts Amherst UMASS. n.d. The flame photometric detector. Accessed 8 Apr 2019.
  85. Vekey, K. 2001. Mass spectrometry and mass-selective detection in chromatography. Journal of Chromatography A 921: 227–236.CrossRefGoogle Scholar
  86. Vincent, A.T., N. Derome, B. Boyle, A.I. Culley, and S.J. Charette. 2017. Next-generation sequencing (NGS) in the microbiological world: How to make the most of your money. Journal of Microbiological Methods 138: 60–71.CrossRefGoogle Scholar
  87. Visser, M., R. Bester, J.T. Burger, and H.J. Maree. 2016. Next-generation sequencing for virus detection: Covering all the bases. Virology Journal 13: 85.CrossRefGoogle Scholar
  88. Vivekanand, L. S. n.d. Radiation surveys, Instrumentation and Dosimetry. NXP Semiconductors. Available at
  89. Voborova, O., M. Bentahir, A.-S. Piette, and J.-L. Gala. 2015. CBRN: Detection and identification innovations. Crisis Response Journal 2015: 36–38.Google Scholar
  90. Walper, S.A., G.L. Aragonés, K.E. Sapsford, et al. 2018. Detecting biothreat agents: From current diagnostics to developing sensor technologies. ACS Sensing 2018 (3): 1894–2024.CrossRefGoogle Scholar
  91. Warwick, B., and B. Dunn. 2011. Chapter 2 – Mass spectrometry in systems biology: An introduction. In Methods in enzymology, vol. 500. Elsevier.Google Scholar
  92. Wirth, K., and A. Barth. n.d. X-ray fluorescence (XRF). The Science Education Research Center at Carleton College. Accessed 8 Apr 2019.
  93. Wunderlich, B. 2001. Thermal analysis. In Encyclopedia of materials: Science and technology, 2nd ed. Elsevier.Google Scholar
  94. Zasada, A.A., K. Forminska, K. Zacharczuk, D. Jacob, and R. Grunow. 2015. Comparison of eleven commercially available rapid tests for detection of bacillus anthracis, francisella tularensis and yersinia pestis. Letters in Applied Microbiology 60: 409–413.CrossRefGoogle Scholar
  95. Zubarev, R.A., and A. Makarov. 2013. Orbitrap mass spectrometry. Analytical Chemistry 85 (11): 5288–5296.CrossRefGoogle Scholar

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Authors and Affiliations

  1. 1.Hotzone Solutions GroupThe HagueThe Netherlands

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