Quantitative Nondestructive Assessment of Paenibacillus larvae in Apis mellifera Hives

  • David LyallEmail author
  • Phil Hansbro
  • Jay Horvat
  • Peter Stanwell
Conference paper
Part of the Advances in Intelligent Systems and Computing book series (AISC, volume 1026)


Paenibacillus larvae is a Gram-positive spore forming bacterium and is the causative agent of American Foulbrood (AFB), a highly contagious infectious disease of Apis Mellifera (the European honey bee), which results in hive death and significant economic losses within the apiary industry world-wide. AFB is typically diagnosed by visual identification which is labour intensive and highly operator dependent. In the absence of a low cost, rapid and reliable industry surveillance tool targeting AFB, the disease has spread around the world. Real-time polymerase chain reaction (rtPCR) is a potential AFB surveillance tool with a demonstrated high sensitivity for the detection and quantification of Paenibacillus larvae infections in Apis Mellifera hives. Current techniques result in the disturbance of the internal hive microclimate by opening and deconstructing the hive. Our research aims to develop a new, non-invasive, rtPCR technique for the detection of AFB in beehives without the need to open the hive.


Paenibacillus larvae American Foulbrood AFB Polymerase Chain Reaction PCR Non-destructive Apis Mellifera European honeybee Bee 


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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • David Lyall
    • 1
    Email author
  • Phil Hansbro
    • 2
  • Jay Horvat
    • 2
  • Peter Stanwell
    • 1
  1. 1.School of Health SciencesThe University of NewcastleCallaghanAustralia
  2. 2.School of Biomedical Sciences and PharmacyThe University of NewcastleCallaghanAustralia

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