Precipitation of Particles Using Combined High Turbulence Extraction Assisted by Ultrasound and Supercritical Antisolvent Fractionation

  • Diego T. SantosEmail author
  • Ádina L. Santana
  • M. Angela A. Meireles
  • Ademir José Petenate
  • Eric Keven Silva
  • Juliana Q. Albarelli
  • Júlio C. F. Johner
  • M. Thereza M. S. Gomes
  • Ricardo Abel Del Castillo Torres
  • Tahmasb Hatami
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)


High Turbulence Extraction Assisted by Ultrasound combined with Supercritical Antisolvent Fractionation (SAF) was done to intensify the recovery of bixin and phenolic compounds from semi-defatted annatto seeds. Annatto seeds are extensively used due to its colorant properties and bioactive potential in human health. Modifications were performed in the SAF experimental apparatus in order to prevent losses of product. Results show that supercritical fractionation resulted in particle yields between 72.2–80.5%, 20–50% phenolics, and 66% bixin.



R. Abel C. Torres thanks Capes for his doctorate assistantship. Ádina L. Santana thanks Capes (88882.305824/2013-01) for her postdoctoral financial support. M. Angela A. Meireles thanks CNPq for the productivity grant (302423/2015-0). The authors acknowledge the financial support from FAPESP (process 2015/13299-0).


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Copyright information

© The Author(s), under exclusive license to Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Diego T. Santos
    • 1
    Email author
  • Ádina L. Santana
    • 2
  • M. Angela A. Meireles
    • 3
  • Ademir José Petenate
    • 4
  • Eric Keven Silva
    • 5
  • Juliana Q. Albarelli
    • 6
  • Júlio C. F. Johner
    • 7
  • M. Thereza M. S. Gomes
    • 8
  • Ricardo Abel Del Castillo Torres
    • 9
  • Tahmasb Hatami
    • 10
  1. 1.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  2. 2.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  3. 3.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  4. 4.Process ImprovementEDTICampinasBrazil
  5. 5.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  6. 6.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  7. 7.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  8. 8.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  9. 9.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil
  10. 10.LASEFI/DEA, School of Food EngineeringUniversity of Campinas—UNICAMPCampinasBrazil

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