Biocatalysis pp 113-138 | Cite as

Immobilization of α-amylases and Their Analytical Applications

  • Om Prakash
  • Saumya Khare


The enzyme stability is a key parameter for its analytical applications. In this backdrop, the current chapter gives an insight about enzyme immobilization using various strategies highlighting its pros and cons. The chapter provides an overview about industrial application of immobilized α-amylase on various matrices with major focus on emerging technologies. The nanotechnology coupled with biotechnological advances provides new avenue in the area of enzyme immobilization. The chapter reveals the excellent properties of nanomaterials as efficient matrices for enzyme immobilization with enhanced catalytic activity, stability and reusability leading the process economically viable and environmentally feasible. The immobilized α-amylase on nanomatrices possess excellent properties contrary to their soluble forms are more preferred for industrial processes. Hence, the present chapter gives an idea about the ongoing research on industrially important α-amylase highlighting new approaches of nanotechnology for enzyme immobilization, which suggests its efficient role for various analytical applications.


Alpha-amylase Enzyme immobilization Nanomaterial matrices Industrial application 



Amino-functionalized silica-coated magnetite nanoparticles




Ag nanoparticles


Ag-nanoparticle-decorated eggshell membrane


Calcium (alginate.starch/polyethyleneimine/glutaraldehyde)


Crosslinked enzyme aggregate




Encapsulated enzyme


Eggshell membrane


Gold nanorods


1-3 (hydroxyethylmethacrylate glycidyl methacrylate)


Isophorone diisocyanate


Layered double-hydroxide


Luffa operculata fibre


Macromolecular cross-linked enzyme aggregates


Magnetic particles immobilization α-amylase


Magnetic Molecular Imprinted Polymer




Nanostructured metal oxides


Porcine pancreatic α-amylase




3-D poly(ethylene-alt-maleic anhydride)


Poly(octadecene-alt-maleic anhydride)


Polypyrrole/silver nanocomposite


Polyurethane urea


Poly(vinyl alcohol)


Poly vinyl alcohol/poly acrylic acid


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Om Prakash
    • 1
  • Saumya Khare
    • 1
  1. 1.Department of Biochemistry, Institute of ScienceBanaras Hindu UniversityVaranasiIndia

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