Cryopreservation of Grapevine Shoot Tips from In Vitro Plants Using Droplet Vitrification and V Cryo-plate Techniques
The availability of and easy access to Vitis genetic resources are essential for future breeding program advances. Cryopreservation is currently considered an ideal means for the long-term preservation of clonally propagated plant genetic resources. When robust methods for cryopreservation of Vitis spp. are available, there is an opportunity to preserve collections for extended lengths of time with minimal cost and labor requirements and a low risk of loss. This chapter describes the droplet vitrification and V cryo-plate protocols that have been shown to be effective for the cryopreservation of multiple V. vinifera genotypes and other Vitis species.
KeywordsVitis Tissue culture Conservation Genetic resources Germplasm
This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the US Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. All opinions expressed in this paper are the author’s and do not necessarily reflect the policies and views of USDA, DOE, or ORAU/ORISE. The authors are grateful to the ORISE/USDA for the Postdoctoral Researcher stipend granted to Jean Carlos Bettoni to perform research at the USDA-ARS National Laboratory for Genetic Resources Preservation (NLGRP). NLGRP Vitis cryopreservation research is funded in part by the Science and Technology Development Fund (Egypt). Ranjith Pathirana acknowledges the funding by Royal Society Te Apārangi for collaboration with USDA under the Catalyst Seeding project CSG-PAF1702.
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