The Staining of Mast Cells

  • Domenico RibattiEmail author


The histochemical techniques for subdividing mast cells stemmed from the observation by Ehrlich’ in 1876 that their lysosomal granules have the capacity to take up and stain metachromatically with basic dyes, such as toluidine blue. Among the histochemical methods to stain mast cells, it is to note to mention Sudan Black B, Luna stain or even Ziehl-Neelsen, which has been assessed historically (Rest and Lee 1979).


  1. Ackroyd W (1876) Metachronism or Colour Change. Chem News 33:60Google Scholar
  2. Benditt EP (1956) An enzyme in mast cells with some properties resembling chymotrypsin. Fed Proc 1956(15):507Google Scholar
  3. Benditt EP, Arase M (1959) An enzyme in mast cells with properties like chymotrypsin. J Exp Med 110:451–460CrossRefGoogle Scholar
  4. Combs JW (1965) Differentiation and proliferation of embryonic mast cells of the rat. J Cell Biol 25:577–592CrossRefGoogle Scholar
  5. D’mello AXP, Sylvester TV, Ramya V et al (2016) Metachromasia and Metachromatic dyes: a review. Int J Adv Health Sci 2:12–17Google Scholar
  6. Dimlich RV, Meineke HA, Reilly FD et al (1980) The fluorescent staining of heparin in mast cells using berberine sulfate: compatibility with paraformaldehyde or o-phthalaldehyde induced fluorescence and metachromasia. Stain Technol 55:217–223CrossRefGoogle Scholar
  7. Ehrlich P (1879) Beiträge zur Kenntnis der Granulierten Bindegewebszellen und der Eosinophilen Leukozyten. Arch Anat Physiol 3:166–169Google Scholar
  8. Ehrlich P (1894) Farbenanalytisehe Untersuchungen zur Histologie und Klinik Des Blutes. DMW—Dtsch med Wochenschr 20:135–136CrossRefGoogle Scholar
  9. Ehrlich PLA (1898) Die Anemie, 1. Normale und Patologische Histologie des Blutes Wien: HolderGoogle Scholar
  10. Enerbäck L (1966a) Mast cells in rat gastrointestinal Mucosa. I. Effects of fixation. Acta Pathol Microbiol Scand 66:289–302CrossRefGoogle Scholar
  11. Enerbäck L (1966b) Mast cells in rat gastrointestinal Mucosa. 2. Dye-binding and metachromatic properties. Acta Pathol Microbiol Scand 66:303–312CrossRefGoogle Scholar
  12. Enerbäck L (1974) Berberine sulphate binding to mast cell polyanions: a cytofluorometric method for the quantitation of heparin. Histochemistry 42:301–313CrossRefGoogle Scholar
  13. Enerbäck L (1986) Mast cell heterogeneity: the evolution of the concept of a specific mucosal mast cell. In: Befus AD, Bienenstock J, Denburg JA (eds) Mast cell differentiation and heterogeneity. Raven Press, New York, pp 1–26Google Scholar
  14. Escribano L, Dı́az-Agustı́n B, Bellas C et al (2001) Utility of flow cytometric analysis of mast cells in the diagnosis and classification of adult mastocytosis. Leuk Res 25:563–70CrossRefGoogle Scholar
  15. Glenner GG, Cohen LA (1960) Histochemical demonstration of a species-specific trypsin-like enzyme in mast cells. Nature 185:846–847CrossRefGoogle Scholar
  16. Gomori G (1953) Chloroacyl esters as histochemical substrates. J Histochem Cytochem 1:469–470CrossRefGoogle Scholar
  17. Holmgren HWO (1937) Beitrag zur Kenntnis der Chemie und Funktion der Ehrlichschen Mastzellen. Z Mikrosk Anat Forsch 42:242–278Google Scholar
  18. Irani AM, Schwartz LB (1989) Mast cell heterogeneity. Clin Exp Allergy 19:143–155CrossRefGoogle Scholar
  19. Irani AM, Schwartz LB (1994) Human mast cell heterogeneity. Allergy Proc 15:303–308CrossRefGoogle Scholar
  20. Irani AA, Schechter NM, Craig SS et al (1986) Two types of human mast cells that have distinct neutral protease compositions. Proc Natl Acad Sci USA 83:4464–4468CrossRefGoogle Scholar
  21. Jorpes EHH, Wilander O (1937) Über das Vorkommen von Heparin in den Gefässwänden und in den Augen. Ein Beitrag zur Physiologie der Ehrlichschen Mastzellen. Z Mikrosk Anat Forsch 42:279–301Google Scholar
  22. Juhlin L, Shelley WB (1966) Detection of histamine by a new fluorescent o-phthalaldehyde stain. J Histochem Cytochem 14:525–528CrossRefGoogle Scholar
  23. Klatt EC, Lujes RJ, Meyer PR (1983) Benign and malignant mast cell proliferations. Diagnosis and separation using a Ph-dependent toluidine blue staining in tissue section. Cancer 51:1119–1124CrossRefGoogle Scholar
  24. Kramer H, Windrum GM (1955) the metachromatic staining reaction. J Histochem Cytochem 3:227–237CrossRefGoogle Scholar
  25. Lammie A, Dobnojak M, Gerald W et al (1994) Expression of C-Kit and kit ligand proteins in normal human tissues. J Histochem Cytochem 42:1417–1425CrossRefGoogle Scholar
  26. Lennert K (1962) Zur pathologischen Anatomie von Urticaria pigmentosa und Mastzellenreticulose. Klin Wochenschr 40:61–67CrossRefGoogle Scholar
  27. Moloney WC, McPherson K, Fliegelman L (1960) Esterase activity in leukocytes demonstrated by the use of naphthol as-D chloroacetate substrate. J Histochemi Cytochemi 8:200–207CrossRefGoogle Scholar
  28. Patruno R, Marech I, Zizzo N et al (2014) c-kit expression, angiogenesis, and grading in canine mast cell tumour: a unique model to study c-kit driven human malignancies. Biomed Res Int 2014:730246CrossRefGoogle Scholar
  29. Qi JC, Li L, Li Y et al (2003) An antibody raised against in vitro-derived human mast cells identifies mature mast cells and a population of cells that are Fc epsilon Ri (+), tryptase (−), and chymase (−) in a variety of human tissues. J Histochem Cytochem 51:643–653CrossRefGoogle Scholar
  30. Rest JR, Lee RL (1979) staining of mast cell granules by the ziehl-neelsen method and differential diagnosis of malignant dermal tumours in the dog. Vet Rec 104:79CrossRefGoogle Scholar
  31. Ribatti D, Crivellato E (2014) Mast cell ontogeny: an historical overview. Immunol Lett 159:11–14CrossRefGoogle Scholar
  32. Ribatti D, Crivellato E, Candussio L et al (2001a) Mast cells and their secretory granules are angiogenic in the chick embryo chorioallantoic membrane. Clin Exp Allergy 31:602–608CrossRefGoogle Scholar
  33. Ribatti D, Vacca A, Nico B et al (2001b) The role of mast cells in tumour angiogenesis. Brit J Haematol 115:514–521CrossRefGoogle Scholar
  34. Ribatti D, Nico B, Finato N et al (2009) Co-localization of tryptase and cathepsin-G in mast cells in cutaneous mastocytosis. Cancer Lett 279:209–212CrossRefGoogle Scholar
  35. Ribatti D, Guidolin D, Marzullo A et al (2010) Mast cells and angiogenesis in gastric carcinoma. Int J Exp Pathol 91:350–356CrossRefGoogle Scholar
  36. Schechter NM, Choi JK, Slavin DA et al (1986) Identification of a chymotrypsin-like proteinase in human mast cells. J Immunol 137:962–970PubMedGoogle Scholar
  37. Schwartz LB, Lewis RA, Austen KF (1981) Tryptase from human pulmonary mast cells. Purification and characterization. J Biol Chem 256:11939–11943PubMedGoogle Scholar
  38. Schwartz LB, Irani AA, Roller K et al (1987) quantitation of histamine, tryptase and chymase in dispersed human T and TC mast cells. J Immunol 138:2611–2615PubMedGoogle Scholar
  39. Schwartz LB, Sakai K, Bradford TR et al (1995) The alpha form of human tryptase is the predominant form present in blood at baseline in normal subjects and is elevated in those with systemic mastocytosis. J Clin Invest 96:2702–2710CrossRefGoogle Scholar
  40. Scott JE, Quintarelli G, Dellovo MC (1964) The chemical and histochemical properties of alcian blue. Histochemie 4:73–85CrossRefGoogle Scholar
  41. Shelley WB, ÖHman S, Parnes HM (1968) Mast cell stain for histamine in freeze-dried embedded tissue. J Histochem Cytochem 16:433–439CrossRefGoogle Scholar
  42. Spicer SS (1960) A correlative study of the histochemical properties of rodent acid mucopolysaccharides. J Histochem Cytochem 8:18–35CrossRefGoogle Scholar
  43. Spicer SS (1963) Histochemical properties of mucopolysaccharixe and basic protein in mast cells. Ann N Y Acad Sci 103:322–333CrossRefGoogle Scholar
  44. Spicer SS, Leppy TJ (1967) Histochemistry of connective tissue mucopolysaccharides in: “The Connective Tissue”. International Academy of Pathology Monograph N7, The Williams and Wilkins Company, Baltimore 1967Google Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Biomedical Sciences, Neurosciences and Sensory OrgansUniversity of BariBariItaly

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