Heat Shock Protein 90 in Kidney Stone Disease
Since early 2000s, proteomics has been applied to kidney stone research by its capability to uncover the previously unexplored or unknown proteins that are involved in kidney stone pathogenesis but had been previously hidden by the limitation of tools for protein science in the past. Using proteomics approach, several novel findings have been generated and a list of proteins involved in kidney stone formation or development has been expanded tremendously. One of such proteins is heat shock protein 90 (HSP90), which is generally known as a molecular chaperone. Its common roles are stress response and regulation of folding and conformation of many other proteins. However, new findings from recent proteomics studies have shown that HSP90 is also involved in kidney stone formation and disease mechanisms as summarized and discussed in this chapter.
KeywordsCalcium oxalate Inflammation Macrophage Monocyte Nephrolithiasis Proteomics
Affinity purification followed by mass spectrometry
Calcium oxalate monohydrate
Heat shock protein 90
Monocyte chemoattractant protein-1
Small interfering RNA targeting to HSP90
Small interfering RNA
Tandem affinity purification
Tumor necrosis factor-α
This work was supported by Mahidol University research grant and the Thailand Research Fund (IRN60W0004 and IRG5980006).
- Jin S, Song YC, Emili A, Sherman PM, Chan VL (2003) JlpA of Campylobacter jejuni interacts with surface-exposed heat shock protein 90alpha and triggers signalling pathways leading to the activation of NF-kappaB and p38 MAP kinase in epithelial cells. Cell Microbiol 5:165–174PubMedCrossRefPubMedCentralGoogle Scholar
- Manissorn J, Singhto N, Thongboonkerd V (2018) Characterizations of HSP90-interacting complex in renal cells using tandem affinity purification and its potential role in kidney stone formation. Proteomics 18:XXXGoogle Scholar
- Okada A, Yasui T, Hamamoto S, Hirose M, Kubota Y, Itoh Y, Tozawa K, Hayashi Y, Kohri K (2009) Genome-wide analysis of genes related to kidney stone formation and elimination in the calcium oxalate nephrolithiasis model mouse: detection of stone-preventive factors and involvement of macrophage activity. J Bone Miner Res 24:908–924PubMedCrossRefPubMedCentralGoogle Scholar
- Okada A, Yasui T, Fujii Y, Niimi K, Hamamoto S, Hirose M, Kojima Y, Itoh Y, Tozawa K, Hayashi Y et al (2010) Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice: detection by association analysis of stone-related gene expression and microstructural observation. J Bone Miner Res 25:2701–2711PubMedCrossRefPubMedCentralGoogle Scholar