Barley, Disease Resistance, and Molecular Breeding Approaches

  • Baljinder Singh
  • Sahil Mehta
  • Sumit Kumar Aggarwal
  • Manish Tiwari
  • Shafiqul Islam Bhuyan
  • Sabhyata Bhatia
  • Md Aminul Islam


At present, the cultivated barley (Hordeum vulgare L.) is among the four major crops produced worldwide used for human food, stews, cattle feed, brewing, and malt production. There is a wide range of biotic variables which affect both quality and quantity components of the multi-nutrient crop. Among the biotic variables, phytopathogens are considered as the most negative constraint on the global barley production. In addition, the intensive monoculture of cultivars along with changing climate conditions has boosted the emergence of new virulent races and pathovars. The earlier conventional breeding efforts were focused mainly on simple genetics, selection, mutation breeding, and hybridization. However, they were ineffective in developing new varieties with durable and broad-spectrum resistance in a short span of time. As a result, the breeders have shifted their focus from conventional approaches to better molecular approaches for enhancing disease resistance in the last 20 years. These better molecular approaches include transgenic technology, VIGS (virus-induced gene silencing), marker-assisted selection (MAS), QTL mapping, gene mapping, and TILLING (targeting induced local lesions in genomes). These approaches have provided novel strategies for enhancing durability and broad-spectrum disease resistance in a short span of time. Furthermore, these technologies have shown tremendous potential to accelerate crop improvement efforts as well as sustained global production, especially for barley. In this chapter, we have focused on the widely employed molecular approaches for enhancing disease resistance in barley (Hordeum vulgare L.).


Barley Cereal Population Yield, disease Genetic resources Powdery mildew Rust QTL mapping Marker-assisted selection Transgenics Gene editing 


  1. Ali MA, Shahzadi M, Zahoor A, Dababat AA, Toktay H, Bakhsh A et al (2019) Resistance to cereal cyst nematodes in wheat and barley: an emphasis on classical and modern approaches. Int J Mol Sci 20:e432PubMedGoogle Scholar
  2. Arru L, Francia E, Pecchioni N (2003) Isolate-specific QTLs of resistance to leaf stripe (Pyrenophora graminea) in the'Steptoe'×'Morex'spring barley cross. Theor Appl Genet 106:668–675PubMedGoogle Scholar
  3. Ashkani S, Rafii MY, Shabanimofrad M, Miah G, Sahebi M, Azizi P et al (2015) Molecular breeding strategy and challenges towards improvement of blast disease resistance in rice crop. Front Plant Sci 6:886PubMedPubMedCentralGoogle Scholar
  4. Babaeizad V, Imani J, Kogel K-H, Eichmann R, Hückelhoven R (2009) Over-expression of the cell death regulator BAX inhibitor-1 in barley confers reduced or enhanced susceptibility to distinct fungal pathogens. Theor Appl Genet 118:455–463PubMedGoogle Scholar
  5. Badr A, Sch R, Rabey HE, Effgen S, Ibrahim H, Pozzi C et al (2000) On the origin and domestication history of barley (Hordeum vulgare). Mol Biol Evol 17:499–510PubMedGoogle Scholar
  6. Barati M, Majidi MM, Mostafavi F, Mirlohi A, Safari M, Karami Z (2018) Evaluation of wild barley species as possible sources of drought tolerance for arid environments. Plant Genet Resour 16:209–217Google Scholar
  7. Bartlett JG, Alves SC, Smedley M, Snape JW, Harwood WA (2008) High-throughput Agrobacterium-mediated barley transformation. Plant Methods 4:22PubMedPubMedCentralGoogle Scholar
  8. Behn A, Hartl L, Schweizer G, Wenzel G, Baumer M (2004) QTL mapping for resistance against non-parasitic leaf spots in a spring barley doubled haploid population. Theor Appl Genet 108:1229–1235PubMedGoogle Scholar
  9. Bellard C, Bertelsmeier C, Leadley P, Thuiller W, Courchamp F (2012) Impacts of climate change on the future of biodiversity. Ecol Lett 15:365–377PubMedPubMedCentralGoogle Scholar
  10. Bilgic H, Steffenson B, Hayes P (2006) Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley. Phytopathology 96:699–708PubMedGoogle Scholar
  11. Boivin NL, Zeder MA, Fuller DQ, Crowther A, Larson G, Erlandson JM et al (2016) Ecological consequences of human niche construction: examining long-term anthropogenic shaping of global species distributions. Proc Natl Acad Sci 113:6388–6396PubMedGoogle Scholar
  12. Brown JK (1994) Chance and selection in the evolution of barley mildew. Trends Microbiol 2:470–475PubMedGoogle Scholar
  13. Budhagatapalli N, Rutten T, Gurushidze M, Kumlehn J, Hensel G (2015) Targeted modification of gene function exploiting homology-directed repair of TALEN-mediated double strand breaks in barley. G3 (Bethesda) 5:1857–1863Google Scholar
  14. Bulgarelli D, Collins N, Tacconi G, Dellaglio E, Brueggeman R, Kleinhofs A et al (2004) High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. Theor Appl Genet 108:1401–1408PubMedGoogle Scholar
  15. Burdon JJ, Laine A-L (2019) Evolutionary dynamics of plant pathogen interactions. Cambridge University Press, CambridgeGoogle Scholar
  16. Cakir M, Gupta S, Li C, Hayden M, Mather DE, Ablett GA et al (2011) Genetic mapping and QTL analysis of disease resistance traits in the barley population Baudin× AC Metcalfe. Crop Pasture Sci 62:152–161Google Scholar
  17. Case AJ, Bhavani S, Macharia G, Pretorius Z, Coetzee V, Kloppers F et al (2018) Mapping adult plant stem rust resistance in barley accessions Hietpas-5 and GAW-79. Theor Appl Genet 131:2245–2266PubMedGoogle Scholar
  18. Castro A, Capettini F, Corey A, Filichkina T, Hayes P, Kleinhofs A et al (2003) Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley. Theor Appl Genet 107:922–930PubMedGoogle Scholar
  19. Castro AJ, Gamba F, German S, Gonzalez S, Hayes PM, Pereyra S et al (2012) Quantitative trait locus analysis of spot blotch and leaf rust resistance in the BCD47× Baronesse barley mapping population. Plant Breed 131:258–266Google Scholar
  20. Cazalis V, Loreau M, Henderson K (2018) Do we have to choose between feeding the human population and conserving nature? Modelling the global dependence of people on ecosystem services. Sci Total Environ 634:1463–1474PubMedGoogle Scholar
  21. Ceccarelli S, Grando S, Hamblin J (1992) Relationship between barley grain yield measured in low-and high-yielding environments. Euphytica 64:49–58Google Scholar
  22. Cejnar P, Ohnoutková L, Ripl J, Vlčko T, Kundu JK (2018) Two mutations in the truncated Rep gene RBR domain delayed the Wheat dwarf virus infection in transgenic barley plants. J Integr Agric 17:2492–2500Google Scholar
  23. Chawla HS (2009) Introduction to plant biotechnology (3/e). CRC Press, Boca RatonGoogle Scholar
  24. Chen G, Liu Y, Ma J, Zheng Z, Wei Y, McIntyre CL et al (2013a) A novel and major quantitative trait locus for Fusarium crown rot resistance in a genotype of wild barley (Hordeum spontaneum L.). PLoS One 8:e58040PubMedPubMedCentralGoogle Scholar
  25. Chen G, Liu Y, Wei Y, McIntyre C, Zhou M, Zheng Y-L et al (2013b) Major QTL for Fusarium crown rot resistance in a barley landrace. Theor Appl Genet 126:2511–2520PubMedGoogle Scholar
  26. Chopra R, Saini R (2014) Transformation of blackgram (Vigna mungo (L.) Hepper) by barley chitinase and ribosome-inactivating protein genes towards improving resistance to Corynespora leaf spot fungal disease. Appl Biochem Biotechnol 174:2791–2800PubMedGoogle Scholar
  27. Christensen AB, Thordal-Christensen H, Zimmermann G, Gjetting T, Lyngkjær MF, Dudler R et al (2004) The germinlike protein GLP4 exhibits superoxide dismutase activity and is an important component of quantitative resistance in wheat and barley. Mol Plant-Microbe Interact 17:109–117PubMedGoogle Scholar
  28. Chutimanitsakun Y, Nipper RW, Cuesta-Marcos A, Cistué L, Corey A, Filichkina T et al (2011) Construction and application for QTL analysis of a Restriction Site Associated DNA (RAD) linkage map in barley. BMC Genomics 12:4PubMedPubMedCentralGoogle Scholar
  29. Comai L, Young K, Till BJ, Reynolds SH, Greene EA, Codomo CA et al (2004) Efficient discovery of DNA polymorphisms in natural populations by Ecotilling. Plant J 37:778–786PubMedGoogle Scholar
  30. Cunniffe NJ, Koskella B, Metcalf CJE, Parnell S, Gottwald TR, Gilligan CA (2015) Thirteen challenges in modelling plant diseases. Epidemics 10:6–10PubMedGoogle Scholar
  31. Dai F, Zhang G (2016) Domestication and improvement of cultivated barley. In: Exploration, identification and utilization of barley germplasm. Academic Press, pp 1–26. Available at Scholar
  32. Dawson AM, Ferguson JN, Gardiner M, Green P, Hubbard A, Moscou MJ (2016) Isolation and fine mapping of Rps6: an intermediate host resistance gene in barley to wheat stripe rust. Theor Appl Genet 129:831–843PubMedPubMedCentralGoogle Scholar
  33. del Blanco IA, Hegarty J, Gallagher L, Falk B, Brown-Guedira G, Pellerin E et al (2014) Mapping of QTL for tolerance to Cereal yellow dwarf virus in two-rowed spring barley. Crop Sci 54:1468–1475PubMedGoogle Scholar
  34. Douchkov D, Lueck S, Hensel G, Kumlehn J, Rajaraman J, Johrde A et al (2016) The barley (Hordeum vulgare) cellulose synthase-like D2 gene (HvCslD2) mediates penetration resistance to host-adapted and nonhost isolates of the powdery mildew fungus. New Phytol 212:421–433PubMedGoogle Scholar
  35. Dracatos PM, Khatkar MS, Singh D, Park RF (2014) Genetic mapping of a new race specific resistance allele effective to Puccinia hordei at the Rph9/Rph12 locus on chromosome 5HL in barley. BMC Plant Biol 14:1598PubMedPubMedCentralGoogle Scholar
  36. Drader T, Kleinhofs A (2010) A synteny map and disease resistance gene comparison between barley and the model monocot Brachypodium distachyon. Genome 53:406–417PubMedGoogle Scholar
  37. Eichmann R, Bischof M, Weis C, Shaw J, Lacomme C, Schweizer P et al (2010) BAX INHIBITOR-1 is required for full susceptibility of barley to powdery mildew. Mol Plant-Microbe Interact 23:1217–1227PubMedGoogle Scholar
  38. Esvelt KK, Gordon T, Bregitzer P, Hayes P, Chen X, Del Blanco I et al (2016) Barley stripe rust resistance QTL: development and validation of SNP markers for resistance to Puccinia striiformis f. sp. hordei. Phytopathology 106:1344–1351Google Scholar
  39. FAOSTAT FPAC (2016) Food and agriculture organization of the United Nations, Roma, p 2010Google Scholar
  40. Francia E, Tondelli A, Rizza F, Badeck FW, Nicosia OLD, Akar T et al (2011) Determinants of barley grain yield in a wide range of Mediterranean environments. Field Crop Res 120:169–178Google Scholar
  41. Frantzeskakis L, Kracher B, Kusch S, Yoshikawa-Maekawa M, Bauer S, Pedersen C et al (2018) Signatures of host specialization and a recent transposable element burst in the dynamic one-speed genome of the fungal barley powdery mildew pathogen. BMC Genomics 19:381PubMedPubMedCentralGoogle Scholar
  42. Friedt W, Horsley RD, Harvey BL, Poulsen DME, Lance RCM, Ceccarelli S, Grando S, Capettini F (2011) Barley breeding history, progress, objectives, and technology. In: Barley: production, improvement, and uses. Blackwell Publishing, pp 160–220. ISBN 9780813801230, 9780470958636Google Scholar
  43. Gottwald S, Bauer P, Komatsuda T, Lundqvist U, Stein N (2009) TILLING in the two-rowed barley cultivar'Barke'reveals preferred sites of functional diversity in the gene HvHox1. BMC Res Notes 2:258PubMedPubMedCentralGoogle Scholar
  44. Graner A, Streng S, Drescher A, Jin Y, Borovkova I, Steffenson B (2000) Molecular mapping of the leaf rust resistance gene Rph7 in barley. Plant Breed 119:389–392Google Scholar
  45. Gresshoff PM (2017) Technology transfer of plant biotechnology. CRC Press. Available at
  46. Grewal T, Rossnagel B, Pozniak C, Scoles G (2008a) Mapping quantitative trait loci associated with barley net blotch resistance. Theor Appl Genet 116:529–539PubMedGoogle Scholar
  47. Grewal TS, Rossnagel BG, Scoles GJ (2008b) Validation of molecular markers for covered smut resistance and marker-assisted introgression of loose and covered smut resistance into hulless barley. Mol Breed 21:37–48Google Scholar
  48. Grewal TS, Rossnagel BG, Scoles GJ (2012) Mapping quantitative trait loci associated with spot blotch and net blotch resistance in a doubled-haploid barley population. Mol Breed 30:267–279Google Scholar
  49. Gupta S, Li C, Loughman R, Cakir M, Platz G, Westcott S et al (2010) Quantitative trait loci and epistatic interactions in barley conferring resistance to net type net blotch (Pyrenophora teres f. teres) isolates. Plant Breed 129:362–368Google Scholar
  50. Haas M, Menke J, Chao S, Steffenson BJ (2016) Mapping quantitative trait loci conferring resistance to a widely virulent isolate of Cochliobolus sativus in wild barley accession PI 466423. Theor Appl Genet 129:1831–1842PubMedGoogle Scholar
  51. Hamwieh A, Alo F, Ahmed S (2018) Molecular tools developed for disease resistant genes in wheat, barley, lentil and chickpea: a review. Arab J Plant Protect 36:50–56Google Scholar
  52. Hanemann A, Schweizer GF, Cossu R, Wicker T, Röder MS (2009) Fine mapping, physical mapping and development of diagnostic markers for the Rrs2 scald resistance gene in barley. Theor Appl Genet 119:1507–1522PubMedGoogle Scholar
  53. Hansson M, Komatsuda T, Stein N, Muehlbauer GJ (2018) Molecular mapping and cloning of genes and QTLs. In: The barley genome. Springer, Cham, pp 139–154. Available at Scholar
  54. Hao Q, Wang W, Han X, Wu J, Lyu B, Chen F et al (2018) Isochorismate-based salicylic acid biosynthesis confers basal resistance to Fusarium graminearum in barley. Mol Plant Pathol 19:1995–2010Google Scholar
  55. Harwood W (2016) Barley as a cereal model for biotechnology applications. In: Jones HD (ed) Biotechnology of major cereals. CABI, Wallingford, pp 80–87Google Scholar
  56. Harwood WA (2019) An introduction to barley: the crop and the model. Springer, Barley, pp 1–5Google Scholar
  57. Hatta MAM, Johnson R, Matny O, Smedley MA, Yu G, Chakraborty S et al (2018) The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley. bioRxiv. Scholar
  58. Hecht VL, Temperton VM, Nagel KA, Rascher U, Postma JA (2016) Sowing density: a neglected factor fundamentally affecting root distribution and biomass allocation of field grown spring barley (Hordeum vulgare L.). Front Plant Sci 7:944PubMedPubMedCentralGoogle Scholar
  59. Hein I, Barciszewska-Pacak M, Hrubikova K, Williamson S, Dinesen M, Soenderby IE et al (2005) Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley. Plant Physiol 138:2155–2164PubMedPubMedCentralGoogle Scholar
  60. Hickey L, Lawson W, Platz G, Dieters M, Arief V, German S et al (2011) Mapping Rph20: a gene conferring adult plant resistance to Puccinia hordei in barley. Theor Appl Genet 123:55–68PubMedGoogle Scholar
  61. Hickey LT, Lawson W, Platz GJ, Fowler RA, Arief V, Dieters M et al (2012) Mapping quantitative trait loci for partial resistance to powdery mildew in an Australian barley population. Crop Sci 52:1021–1032Google Scholar
  62. Hofmann K, Silvar C, Casas AM, Herz M, Büttner B, Gracia MP et al (2013) Fine mapping of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces. Theor Appl Genet 126:3091–3102PubMedGoogle Scholar
  63. Holzberg S, Brosio P, Gross C, Pogue GP (2002) Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J 30:315–327PubMedGoogle Scholar
  64. Hori K, Sato K, Kobayashi T, Takeda K (2006) QTL analysis of Fusarium head blight severity in recombinant inbred population derived from a cross between two-rowed barley varieties. Breed Sci 56:25–30Google Scholar
  65. Horler R, Turner A, Fretter P, Ambrose M (2017) SeedStor: a germplasm information management system and public database. Plant Cell Physiol 59:e5–e5PubMedCentralGoogle Scholar
  66. Horsley RD, Schmierer D, Maier C, Kudrna D, Urrea CA, Steffenson BJ et al (2006) Identification of QTLs associated with Fusarium head blight resistance in barley accession CIho 4196. Crop Sci 46:145–156Google Scholar
  67. Horvath H, Rostoks N, Brueggeman R, Steffenson B, Von Wettstein D, Kleinhofs A (2003) Genetically engineered stem rust resistance in barley using the Rpg1 gene. Proc Natl Acad Sci 100:364–369PubMedGoogle Scholar
  68. Hu X, Qi X-l, Lv B, J-j W, D-l F (2012) TILLING-based analysis of disease resistance genes in barley [J]. Journal of Shandong Agricultural University (Natural Science Edition) 1:002Google Scholar
  69. Huang Y, Haas M, Heinen S, Steffenson BJ, Smith KP, Muehlbauer GJ (2018) QTL mapping of fusarium head blight and correlated agromorphological traits in an elite barley cultivar Rasmusson. Front Plant Sci 9:1260PubMedPubMedCentralGoogle Scholar
  70. Hudcovicová M, Šudyová V, Šliková S, Gregová E, Kraic J, Ordon F et al (2008) Marker-assisted selection for the development of improved barley and wheat lines. Acta Agronomica Hungarica 56:385–392Google Scholar
  71. Jefferies S, King B, Barr A, Warner P, Logue S, Langridge P (2003) Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley. Plant Breed 122:52–56Google Scholar
  72. Jena KK, Mackill DJ (2008) Molecular markers and their use in marker-assisted selection in rice. Crop Sci 48:1266–1276Google Scholar
  73. Jost M, Szurman-Zubrzycka M, Gajek K, Szarejko I, Stein N (2019) TILLING in barley. Springer, Barley, pp 73–94Google Scholar
  74. Kai H, Takata K, Tsukazaki M, Furusho M, Baba T (2012) Molecular mapping of Rym17, a dominant and rym18 a recessive barley yellow mosaic virus (BaYMV) resistance genes derived from Hordeum vulgare L. Theor Appl Genet 124:577–583PubMedGoogle Scholar
  75. Käsbauer CL, Pathuri IP, Hensel G, Kumlehn J, Hückelhoven R, Proels RK (2018) Barley ADH-1 modulates susceptibility to Bgh and is involved in chitin-induced systemic resistance. Plant Physiol Biochem 123:281–287PubMedGoogle Scholar
  76. Kis A, Hamar É, Tholt G, Bán R, Havelda Z (2019) Creating highly efficient resistance against Wheat dwarf virus in barley by employing CRISPR/Cas9 system. Plant Biotechnol J. Scholar
  77. Kis A, Tholt G, Ivanics M, Várallyay É, Jenes B, Havelda Z (2016) Polycistronic artificial miRNA-mediated resistance to W heat dwarf virus in barley is highly efficient at low temperature. Mol Plant Pathol 17:427–437PubMedGoogle Scholar
  78. König J, Kopahnke D, Steffenson B, Przulj N, Romeis T, Röder M et al (2012) Genetic mapping of a leaf rust resistance gene in the former Yugoslavian barley landrace MBR1012. Mol Breed 30:1253–1264Google Scholar
  79. Lawrenson T, Shorinola O, Stacey N, Li C, Østergaard L, Patron N et al (2015) Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol 16:258PubMedPubMedCentralGoogle Scholar
  80. Le Gouis J, Devaux P, Werner K, Hariri D, Bahrman N, Beghin D et al (2004) RYM15 from the Japanese cultivar Chikurin Ibaraki 1 is a new barley mild mosaic virus (BaMMV) resistance gene mapped on chromosome 6H. Theor Appl Genet 108:1521–1525PubMedGoogle Scholar
  81. Leckband G, Lörz H (1998) Transformation and expression of a stilbene synthase gene of Vitis vinifera L. in barley and wheat for increased fungal resistance. Theor Appl Genet 96:1004–1012Google Scholar
  82. Lee S, Neate S (2007) Molecular mapping of Rsp 1, Rsp 2, and Rsp 3 genes conferring resistance to Septoria speckled leaf blotch in barley. Phytopathology 97:155–161PubMedGoogle Scholar
  83. Leng Y, Zhao M, Wang R, Steffenson BJ, Brueggeman RS, Zhong S (2018) The gene conferring susceptibility to spot blotch caused by Cochliobolus sativus is located at the Mla locus in barley cultivar Bowman. Theor Appl Genet 131:1531–1539PubMedGoogle Scholar
  84. Li C, Gupta S, Zhang X-Q, Westcott S, Yang J, Park R et al (2013) A major QTL controlling adult plant resistance for barley leaf rust. In: Advance in barley sciences. Springer, Dordrecht, pp 285–300Google Scholar
  85. Li H, Zhou M (2011) Quantitative trait loci controlling barley powdery mildew and scald resistances in two different barley doubled haploid populations. Mol Breed 27:479–490Google Scholar
  86. Li H, Zhou M, Liu C (2009) A major QTL conferring crown rot resistance in barley and its association with plant height. Theor Appl Genet 118:903–910PubMedGoogle Scholar
  87. Li J, Huang X, Heinrichs F, Ganal M, Röder M (2006) Analysis of QTLs for yield components, agronomic traits, and disease resistance in an advanced backcross population of spring barley. Genome 49:454–466PubMedGoogle Scholar
  88. Lightfoot DJ, Mcgrann GR, Able AJ (2017) The role of a cytosolic superoxide dismutase in barley–pathogen interactions. Mol Plant Pathol 18:323–335PubMedGoogle Scholar
  89. Lindley J, Techera EJ, Webster D (2019) 11 Extreme human behaviours affecting marine resources and industries. Marine Extremes: Ocean Safety, Marine Health and the Blue Economy 63Google Scholar
  90. Liu BH (2017) Statistical genomics: linkage, mapping, and QTL analysis. CRC press, Boca RatonGoogle Scholar
  91. Looseley M, Newton A, Atkins SD, Fitt BD, Fraaije B, Thomas W et al (2012) Genetic basis of control of Rhynchosporium secalis infection and symptom expression in barley. Euphytica 184:47–56Google Scholar
  92. Ma Z, Lapitan NL, Steffenson B (2004) QTL mapping of net blotch resistance genes in a doubled-haploid population of six-rowed barley. Euphytica 137:291–296Google Scholar
  93. Mace E (2016) Molecular biology support for barley improvement-North. Available at
  94. Maguire K, Charlton W, Yoxall T, Burnett F. (2018) The challenges of managing multiple barley pathogens in winter and spring barley. The Dundee Conference. Crop Production in Northern Britain 2018, Dundee, UK, 27–28 February 2018. The Association for Crop Protection in Northern Britain. p. 73–78Google Scholar
  95. Mall T, Gupta M, Dhadialla TS, Rodrigo S (2019) Overview of biotechnology-derived herbicide tolerance and insect resistance traits in plant agriculture. In: Kumar S, Barone P, Smith M (eds) Transgenic plants: methods and protocols. Springer New York, New York, pp 313–342Google Scholar
  96. Mammadov J, Zwonitzer J, Biyashev R, Griffey C, Jin Y, Steffenson B et al (2003) Molecular mapping of leaf rust resistance gene Rph 5 in barley. Crop Sci 43:388–393Google Scholar
  97. Manninen O, Jalli M, Kalendar R, Schulman A, Afanasenko O, Robinson J (2006) Mapping of major spot-type and net-type net-blotch resistance genes in the Ethiopian barley line CI 9819. Genome 49:1564–1571PubMedGoogle Scholar
  98. Manoharan M, Dahleen LS, Hohn TM, Neate SM, Yu X-H, Alexander NJ et al (2006) Expression of 3-OH trichothecene acetyltransferase in barley (Hordeum vulgare L.) and effects on deoxynivalenol. Plant Sci 171:699–706Google Scholar
  99. Mascher M, Gundlach H, Himmelbach A, Beier S, Twardziok SO, Wicker T et al (2017) A chromosome conformation capture ordered sequence of the barley genome. Nature 544:427–433PubMedGoogle Scholar
  100. McCallum CM, Comai L, Greene EA, Henikoff S (2000a) Targeted screening for induced mutations. Nat Biotechnol 18:455–457PubMedGoogle Scholar
  101. McCallum CM, Comai L, Greene EA, Henikoff S (2000b) Targeting induced local lesions in genomes (TILLING) for plant functional genomics. Plant Physiol 123:439–442PubMedPubMedCentralGoogle Scholar
  102. Miedaner T, Korzun V (2012) Marker-assisted selection for disease resistance in wheat and barley breeding. Phytopathology 102:560–566PubMedPubMedCentralGoogle Scholar
  103. Milne RJ, Dibley KE, Schnippenkoetter WH, Mascher M, Lui AC, Wang L et al (2018) The wheat Lr67 gene of the Sugar Transport Protein family confers multipathogen resistance in barley. Plant Physiol:00945.02018. Scholar
  104. Miyazaki C, Osanai E, Saeki K, Ito K, Konishi T, Sato K et al (2001) Mapping of quantitative trait loci conferring resistance to barley yellow mosaic virus in a Chinese barley landrace Mokusekko 3. Breed Sci 51:171–177Google Scholar
  105. Mohamed A, Ali R, Elhassan O, Suliman E, Mugoya C, Masiga CW et al (2014) First products of DNA marker-assisted selection in sorghum released for cultivation by farmers in sub-saharan Africa. J Plant Sci Mol Breed 3:1–10Google Scholar
  106. Montanari A, Ceola S, Laio F. (2017) Increasing human pressure on freshwater resources threatens sustainability at the global scale. AGU Fall Meeting AbstractsGoogle Scholar
  107. Mundt CC (2014) Durable resistance: a key to sustainable management of pathogens and pests. Infect Genet Evol 27:446–455PubMedPubMedCentralGoogle Scholar
  108. Nelson R, Wiesner-Hanks T, Wisser R, Balint-Kurti P (2018) Navigating complexity to breed disease-resistant crops. Nat Rev Genet 19:21PubMedPubMedCentralGoogle Scholar
  109. Niks R, Habekuss A, Bekele B, Ordon F (2004) A novel major gene on chromosome 6H for resistance of barley against the barley yellow dwarf virus. Theor Appl Genet 109:1536–1543PubMedGoogle Scholar
  110. Nowara D, Gay A, Lacomme C, Shaw J, Ridout C, Douchkov D et al (2010) HIGS: host-induced gene silencing in the obligate biotrophic fungal pathogen Blumeria graminis. Plant Cell 22:3130–3141PubMedPubMedCentralGoogle Scholar
  111. Oerke EC (2005) Crop losses to pests. J Agric Sci 144:31–43Google Scholar
  112. Oliver R (2019) Integrated disease management of wheat and barley. Burleigh Dodds Science Publishing Limited. Available at
  113. Ordon F, Bauer E, Graner A (1995) Marker-based selection for the ym4 BaMMV-resistance gene in barley using RAPDs. Agronomie 15:481–485Google Scholar
  114. Pathak MD, Khan ZR (1994) Insect pests of rice. International Rice Research Institute, Los BanosGoogle Scholar
  115. Perovic D, Kopahnke D, Habekuss A, Ordon F, Serfling A (2019) Marker-based harnessing of genetic diversity to improve resistance of barley to fungal and viral diseases. In: Applications of genetic and genomic research in cereals. Woodhead Publishing, pp 137–164. Available at Scholar
  116. Pessarakli M (2016) Handbook of plant and crop stress. CRc press, Boca RatonGoogle Scholar
  117. Pickering R, Ruge-Wehling B, Johnston P, Schweizer G, Ackermann P, Wehling P (2006) The transfer of a gene conferring resistance to scald (Rhynchosporium secalis) from Hordeum bulbosum into H. vulgare chromosome 4HS. Plant Breed 125:576–579Google Scholar
  118. Pliego C, Nowara D, Bonciani G, Gheorghe DM, Xu R, Surana P et al (2013) Host-induced gene silencing in barley powdery mildew reveals a class of ribonuclease-like effectors. Mol Plant-Microbe Interact 26:633–642PubMedGoogle Scholar
  119. Qi X-l, Xu Z-b, Pei H-c, Hu X, Wu J-j, Li X-b et al (2012) Construction and functional evaluation of an EMS-induced mutant population in barley [J]. J Triticeae Crops 5:008Google Scholar
  120. Ragimekula N, Varadarajula NN, Mallapuram SP, Gangimeni G, Reddy RK, Kondreddy HR (2013) Marker assisted selection in disease resistance breeding. J Plant Breed Genet 1:90–109Google Scholar
  121. Rahnamaeian M, Langen G, Imani J, Khalifa W, Altincicek B, Von Wettstein D et al (2009) Insect peptide metchnikowin confers on barley a selective capacity for resistance to fungal ascomycetes pathogens. J Exp Bot 60:4105–4114PubMedPubMedCentralGoogle Scholar
  122. Rahnamaeian M, Vilcinskas A (2012) Defense gene expression is potentiated in transgenic barley expressing antifungal peptide metchnikowin throughout powdery mildew challenge. J Plant Res 125:115–124PubMedGoogle Scholar
  123. Rajaraman J, Douchkov D, Hensel G, Stefanato FL, Gordon A, Ereful N et al (2016) An LRR/malectin receptor-like kinase mediates resistance to non-adapted and adapted powdery mildew fungi in barley and wheat. Front Plant Sci 7:1836PubMedPubMedCentralGoogle Scholar
  124. Rehman S (2018) Proceedings of the 2nd international workshop on barley leaf diseasesGoogle Scholar
  125. Richards JK, Friesen TL, Brueggeman RS (2017) Association mapping utilizing diverse barley lines reveals net form net blotch seedling resistance/susceptibility loci. Theor Appl Genet 130:915–927PubMedGoogle Scholar
  126. Richardson K, Vales M, Kling J, Mundt C, Hayes P (2006) Pyramiding and dissecting disease resistance QTL to barley stripe rust. Theor Appl Genet 113:485–495PubMedGoogle Scholar
  127. Risk JM, Selter LL, Chauhan H, Krattinger SG, Kumlehn J, Hensel G et al (2013) The wheat L r34 gene provides resistance against multiple fungal pathogens in barley. Plant Biotechnol J 11:847–854PubMedGoogle Scholar
  128. Ritala A, Aspegren K, Kurtén U, Salmenkallio-Marttila M, Mannonen L, Hannus R et al (1994) Fertile transgenic barley by particle bombardment of immature embryos. Plant Mol Biol 24:317–325PubMedGoogle Scholar
  129. Roberts D, Mattoo A (2018) Sustainable agriculture—Enhancing environmental benefits, food nutritional quality and building crop resilience to abiotic and biotic stresses. Agriculture 8:8Google Scholar
  130. Romero CC, Vermeulen JP, Vels A, Himmelbach A, Mascher M, Niks RE (2018) Mapping resistance to powdery mildew in barley reveals a large-effect nonhost resistance QTL. Theor Appl Genet 131:1031–1045PubMedPubMedCentralGoogle Scholar
  131. Rose DC, Sutherland WJ, Barnes AP, Borthwick F, Ffoulkes C, Hall C et al (2019) Integrated farm management for sustainable agriculture: lessons for knowledge exchange and policy. Land Use Policy 81:834–842Google Scholar
  132. Ruge B, Linz A, Pickering R, Proeseler G, Greif P, Wehling P (2003) Mapping of Rym14 Hb, a gene introgressed from Hordeum bulbosum and conferring resistance to BaMMV and BaYMV in barley. Theor Appl Genet 107:965–971PubMedGoogle Scholar
  133. Russell J, Mascher M, Dawson IK, Kyriakidis S, Calixto C, Freund F et al (2016) Exome sequencing of geographically diverse barley landraces and wild relatives gives insights into environmental adaptation. Nat Genet 48:1024–1030PubMedGoogle Scholar
  134. Saisho D, Takeda K (2011) Barley: emergence as a new research material of crop science. Plant Cell Physiol 52:724–727PubMedGoogle Scholar
  135. Sánchez-Vallet A, Fouché S, Fudal I, Hartmann FE, Soyer JL, Tellier A et al (2018) The genome biology of effector gene evolution in filamentous plant pathogens. Annu Rev Phytopathol 56:21–40PubMedGoogle Scholar
  136. Sandhu K, Forrest K, Kong S, Bansal U, Singh D, Hayden M et al (2012) Inheritance and molecular mapping of a gene conferring seedling resistance against Puccinia hordei in the barley cultivar Ricardo. Theor Appl Genet 125:1403–1411PubMedGoogle Scholar
  137. Savary S, Ficke A, Aubertot J-N, Hollier C (2012) Crop losses due to diseases and their implications for global food production losses and food security. Food Security 4(2):519–537Google Scholar
  138. Sayed H, Baum M (2018) Marker-assisted selection for scald (Rhynchosporium commune L.) resistance gene (s) in barley breeding for dry areas. J Plant Protect Res 58:335–344Google Scholar
  139. Schultheiss H, Hensel G, Imani J, Broeders S, Sonnewald U, Kogel K-H et al (2005) Ectopic expression of constitutively activated RACB in barley enhances susceptibility to powdery mildew and abiotic stress. Plant Physiol 139:353–362PubMedPubMedCentralGoogle Scholar
  140. Serna-Saldivar SO (2016) Cereal grains: properties, processing, and nutritional attributes. CRC press. Available at
  141. Shakoor N, Lee S, Mockler TC (2017) High throughput phenotyping to accelerate crop breeding and monitoring of diseases in the field. Curr Opin Plant Biol 38:184–192Google Scholar
  142. Shtaya M, Marcel T, Sillero JC, Niks RE, Rubiales D (2006) Identification of QTLs for powdery mildew and scald resistance in barley. Euphytica 151:421–429Google Scholar
  143. Sieling K, Christen O (2015) Crop rotation effects on yield of oilseed rape, wheat and barley and residual effects on the subsequent wheat. Arch Agron Soil Sci 61:1531–1549Google Scholar
  144. Silvar C, Dhif H, Igartua E, Kopahnke D, Gracia MP, Lasa JM et al (2010) Identification of quantitative trait loci for resistance to powdery mildew in a Spanish barley landrace. Mol Breed 25:581–592Google Scholar
  145. Singh D, Dracatos P, Derevnina L, Zhou M, Park RF (2015) Rph23: a new designated additive adult plant resistance gene to leaf rust in barley on chromosome 7H. Plant Breed 134:62–69Google Scholar
  146. Soldanova M, Ištvánek J, Řepková J, Dreiseitl A (2013) Newly discovered genes for resistance to powdery mildew in the subtelomeric region of the short arm of barley chromosome 7H. Czech J Genet Plant Breed 49:95–102Google Scholar
  147. Srivastava J, Damania A (1989) Use of collections in cereal improvement in semi-arid areas. The use of plant genetic resources. Cambridge University Press, Cambridge, pp 88–104Google Scholar
  148. St. Pierre S, Gustus C, Steffenson B, Dill-Macky R, Smith K (2010) Mapping net form net blotch and Septoria speckled leaf blotch resistance loci in barley. Phytopathology 100:80–84PubMedGoogle Scholar
  149. Stein N, Mascher M (2018) Barley genome sequencing and assembly—a first version reference sequence. In: The barley genome. Springer, Cham, pp 57–71. Avaialble at Scholar
  150. Stenberg JA, Heil M, Åhman I, Björkman C (2015) Optimizing crops for biocontrol of pests and disease. Trends Plant Sci 20:698–712PubMedGoogle Scholar
  151. Sui X, He Z, Lu Y, Wang Z, Xia X (2010) Molecular mapping of a non-host resistance gene YrpstY1 in barley (Hordeum vulgare L.) for resistance to wheat stripe rust. Hereditas 147:176–182PubMedGoogle Scholar
  152. Szurman-Zubrzycka ME, Zbieszczyk J, Marzec M, Jelonek J, Chmielewska B, Kurowska MM et al (2018) HorTILLUS—a rich and renewable source of induced mutations for forward/reverse genetics and pre-breeding programs in barley (Hordeum vulgare L.). Front Plant Sci 9:216PubMedPubMedCentralGoogle Scholar
  153. Tacconi G, Cattivelli L, Faccini N, Pecchioni N, Stanca A, Vale G (2001) Identification and mapping of a new leaf stripe resistance gene in barley (Hordeum vulgare L.). Theor Appl Genet 102:1286–1291Google Scholar
  154. Talamè V, Bovina R, Sanguineti MC, Tuberosa R, Lundqvist U, Salvi S (2008) TILLMore, a resource for the discovery of chemically induced mutants in barley. Plant Biotechnol J 6:477–485PubMedGoogle Scholar
  155. The International Barley Genome Sequencing C, Mayer KFX, Waugh R, Langridge P, Close TJ, Wise RP et al (2012) A physical, genetic and functional sequence assembly of the barley genome. Nature 491:711–716Google Scholar
  156. Toojinda T, Broers L, Chen X, Hayes P, Kleinhofs A, Korte J et al (2000) Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare). Theor Appl Genet 101:580–589Google Scholar
  157. Travella S, Ross S, Harden J, Everett C, Snape J, Harwood W (2005) A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques. Plant Cell Rep 23:780–789PubMedGoogle Scholar
  158. Wagner C, Schweizer G, Krämer M, Dehmer-Badani A, Ordon F, Friedt W (2008) The complex quantitative barley–Rhynchosporium secalis interaction: newly identified QTL may represent already known resistance genes. Theor Appl Genet 118:113–122PubMedGoogle Scholar
  159. Walls J, Rajotte E, Rosa C (2019) The past, present, and future of barley yellow dwarf management. Agriculture 9:23Google Scholar
  160. Wang M-B, Abbott DC, Upadhyaya NM, Jacobsen JV, Waterhouse PM (2001) Agrobacterium tumefaciens-mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes. Funct Plant Biol 28:149–156Google Scholar
  161. Wang R, Leng Y, Zhao M, Zhong S (2018) Fine mapping of a dominant gene conferring resistance to spot blotch caused by a new pathotype of Bipolaris sorokiniana in barley. Theor Appl Genet 132:41–51PubMedGoogle Scholar
  162. Wang Y, Ren X, Sun D, Sun G (2015) Origin of worldwide cultivated barley revealed by NAM-1 gene and grain protein content. Front Plant Sci 6:803PubMedPubMedCentralGoogle Scholar
  163. Wiesner-Hanks T, Nelson R (2016) Multiple disease resistance in plants. Annu Rev Phytopathol 54:229–252Google Scholar
  164. Wonneberger R, Ficke A, Lillemo M (2017) Mapping of quantitative trait loci associated with resistance to net form net blotch (Pyrenophora teres f. teres) in a doubled haploid Norwegian barley population. PLoS One 12:e0175773PubMedPubMedCentralGoogle Scholar
  165. Yan G, Chen X (2006) Molecular mapping of a recessive gene for resistance to stripe rust in barley. Theor Appl Genet 113:529–537PubMedGoogle Scholar
  166. Yu G, Franckowiak J, Neate S, Zhang B, Horsley R (2010) A native QTL for Fusarium head blight resistance in North American barley (Hordeum vulgare L.) independent of height, maturity, and spike type loci. Genome 53:111–118PubMedGoogle Scholar
  167. Yu X, Kong HY, Meiyalaghan V, Casonato S, Chng S, Jones EE et al (2018) Genetic mapping of a barley leaf rust resistance gene Rph26 introgressed from Hordeum bulbosum. Theor Appl Genet 131:2567–2580PubMedGoogle Scholar
  168. Zang W, Eckstein PE, Colin M, Voth D, Himmelbach A, Beier S et al (2015) Fine mapping and identification of a candidate gene for the barley Un8 true loose smut resistance gene. Theor Appl Genet 128:1343–1357PubMedGoogle Scholar
  169. Zhong S, Toubia-Rahme H, Steffenson BJ, Smith KP (2006) Molecular mapping and marker-assisted selection of genes for septoria speckled leaf blotch resistance in barley. Phytopathology 96:993–999PubMedGoogle Scholar
  170. Ziems LA, Hickey LT, Platz GJ, Franckowiak JD, Dracatos PM, Singh D et al (2017) Characterization of Rph24: a gene conferring adult plant resistance to Puccinia hordei in barley. Phytopathology 107:834–841PubMedGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Baljinder Singh
    • 1
  • Sahil Mehta
    • 2
  • Sumit Kumar Aggarwal
    • 3
  • Manish Tiwari
    • 1
  • Shafiqul Islam Bhuyan
    • 4
  • Sabhyata Bhatia
    • 1
  • Md Aminul Islam
    • 1
  1. 1.National Institute of Plant Genome ResearchNew DelhiIndia
  2. 2.International Centre for Genetic Engineering and BiotechnologyNew DelhiIndia
  3. 3.ICAR- Indian Institute of Maize ResearchLudhianaIndia
  4. 4.Department of BotanyPandit Deendayal Upadhayay Aadarsha Mahavidyalaya, BehaliBiswanathIndia

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