Fungal Inoculants for Native Phosphorus Mobilization

  • J. C. Tarafdar
Part of the Soil Biology book series (SOILBIOL, volume 55)


More than 96% of the total native phosphorus present in any agricultural soils is in unavailable inorganic or organic forms. They may be utilized by the plants through the activity of efficient fungi which are secreting/producing/releasing huge amount of acid phosphatase, alkaline phosphatase, phytase, and organic acids. The important fungi capable of doing the job are in the groups of Aspergillus, Emericella, Gliocladium, Penicillium, Trichoderma, and Chaetomium besides some AM fungi like Glomus and Gigaspora. The three efficient fungi already used as inoculums are Chaetomium globosum, Penicillium purpurogenum, and Emericella rugulosa. Seed inoculation using these fungi is mobilizing 45–60 kg P and 16–25% increase in yield of different crops. They are mainly exploiting from labile and moderately labile fractions of phosphorus. Minimum concentration of organic acid of fungal origin required to solubilize P was found between 0.2 and 0.5 mM. In fungal-inoculated plants, microbial contribution was more than the plant contribution. Fungal extracellular enzymes were more efficient than their intracellular counterpart. P uptake occurs around the root tip into epidermal cells with their associated root hairs and into cells in the outer layers of the root cortex. Phosphate can also be taken up by transfer from mycorrhizal fungi to root cortical cells.


Fungal phosphatases and phytases Fungal enzymes Mycorrhizal fungi Mineral nutrition 


  1. Anderson G (1980) Assessing organic phosphorus in soils. In: Khasawneh FE, Sample EC, Kamprath EJ (eds) The role of phosphorus in agriculture. American Society of Agronomy, Madison, pp 411–432Google Scholar
  2. Barber SA (1995) Soil nutrient bioavailability: a mechanistic approach, 2nd edn. Wiley, New York, p 414Google Scholar
  3. Bardiya SA, Gaur AC (1972) Rock phosphate dissolution by bacteria. Indian J Microbiol 12:269–271Google Scholar
  4. Batjes NH (1997) A world data set of derived soil properties by FAO-UNESCO soil unit for global modeling. Soil Use Manag 13:9–16CrossRefGoogle Scholar
  5. Beissner L, Roemer W (1996) Improving the availability of phytase phosphorus to sugar beet (Beta vulgaries L.) by phytase application in soil. Proc Intern Call Plant Nutr, Prague, pp 327–332Google Scholar
  6. Cantrell IC, Linderman RG (2001) Preinoculation of lettuce and onion with VA mycorrhizal fungi reduces deleterious effects and soil salinity. Plant Soil 233:269–281CrossRefGoogle Scholar
  7. Findenegg GR, Neiemans JA (1993) The effect of phytase on the availability of P from myo-inositol hexaphosphate (phytate) for maize roots. Plant Soil 154:189–196CrossRefGoogle Scholar
  8. Foehse D, Claassen N, Jungk A (1988) Phosphorus efficiency of plants. I. External and internal requirement of P uptake efficiency of different plant species. Plant Soil 110:101–109CrossRefGoogle Scholar
  9. Frossard E, Condron LM, Oberson A, Sinaj S, Fardeau JC (2000) processes governing phosphorus availability in temperate soils. J Environ Qual 29:12–53CrossRefGoogle Scholar
  10. Gaume A (2000) Low-P tolerance of various P cultivars: the contribution of the root exudation. PhD Dissertation, Swiss Federal Institute of Technology, Zuerich, SwitzerlandGoogle Scholar
  11. Gharu A, Tarafdar JC (2016) Efficiency of phosphatases in mobilization of native phosphorus fractions under different vegetation. Agric Res 5:335–345CrossRefGoogle Scholar
  12. Greiner R, Konietzny U (2006) Phytase for food application. Food Technol Biotechnol 44:125–140Google Scholar
  13. Guimaraes LHS, Terenzi JA, Jorge FA, Leone ML, Polozeli TM (2003) Extracellular alkaline phosphatase from filamentous fungus Aspergillus caespitosus: purification and biochemical characterization. Folia Microbiol 48:627–632CrossRefGoogle Scholar
  14. Harrison MJ (1999) Molecular and cellular aspects of arbuscular mycorrhizal symbiosis. Ann Rev Plant Physiol Plant Mol Biol 50:361–389CrossRefGoogle Scholar
  15. Harrison MJ, Van Buuren ML (1995) A phosphate transporter from the mycorrhizal fungus Glomus versiforme. Nature 378:626–629CrossRefGoogle Scholar
  16. Haussling M, Marschner H (1989) Organic and inorganic soil phosphates and acid phosphatase activity in the rhizospheres of 80-year-old Norway spruce (Picea abies (L.) Karst.) trees. Biol Fertil Soils 8:128–133CrossRefGoogle Scholar
  17. Hedley MJ, Nye PH, White RE (1982) Plant induced changes in the rhizospheres of rape (Brassica napus var. Emerald) seedlings. II. Origin of the pH change. New Phytol 91:31–44CrossRefGoogle Scholar
  18. Hinsinger P (1998) How do plant roots acquire mineral nutrients? Chemical processes involved in the rhizospheres. Adv Agron 64:225–265CrossRefGoogle Scholar
  19. Hisinger P, Gilkes RJ (1997) Dissolution of phosphate rock in the rhizospheres of five plant species grown in an acid, P-fixing mineral substrate. Geoderma 75:231–249CrossRefGoogle Scholar
  20. Huebel F, Beck E (1993) In-situ determination of the P-relations around the primary root of maize with respect to inorganic and phytate-P. Plant Soil 157:1–9CrossRefGoogle Scholar
  21. Koide TR, Schreiner PR (1992) Regulation of vesicular-arbuscular mycorrhizal symbiosis. Ann Rev Plant Physiol Plant Mol Biol 43:557–581CrossRefGoogle Scholar
  22. Kucey RMN (1988) Effect of Penicillium billai on the solubility and uptake of P and micronutrients from soil by wheat. Can J Soil Sci 68:261–270CrossRefGoogle Scholar
  23. Kucey RMN, Jenzen HH, Leggett ME (1989) Microbially mediated increases in plant available phosphorus. Adv Agron 42:199–228CrossRefGoogle Scholar
  24. Lapeyrie F, Rangers J, Vairelles D (1991) Phosphate solubilizing activity of ecto mycorrhizal fungi in vitro. Can J Bot 69:342–346CrossRefGoogle Scholar
  25. Moose B (1980) Vesicular–arbuscular mycorrhizal research for tropical agriculture. Research Bulletin 194, Hawaii Institute of Tropical Agriculture and Human Resources, University of Hawaii, Honolulu, HIGoogle Scholar
  26. Mosse B (1973) Advances in the study of vesicular arbuscular mycorrhiza. Annu Rev Phytopathol 11:171–196CrossRefGoogle Scholar
  27. Neumann G, Roemheld V (2000) The release of root exudates as affected by the plant physiological status. In: Pinton R, Varanini Z, Nannipieri P (eds) The rhizospheres-biochemistry and organic substances at the soil plant interface. Dekker, New York, pp 41–93Google Scholar
  28. Pant HK, Warman PR (2000) Enzymatic hydrolysis of soil organic phosphorus by immobilized phosphates. Biol Fertil Soils 30:306–311CrossRefGoogle Scholar
  29. Paul NB, Sundara Rao WVB (1971) Phosphate dissolving bacteria and VAM fungi in the rhizospheres of some cultivated legumes. Plant Soil 35:127–132CrossRefGoogle Scholar
  30. Richardson AE (1994) Soil microorganisms and phosphate availability. In: Pankhurst CE, Doulse BM, Gupta VVSR, Grace PR (eds) Soil biota management in sustainable farming systems. CSIRO, Melbourne, pp 50–62Google Scholar
  31. Richardson AEPA, Hadobas JE, Hayes CP, Hara O, Simpson RJ (2001) Utilization of phosphorus and pasture plants supplied with myo-inositol hexaphosphate is enhanced by the presence of soil microorganisms. Plant Soil 229:47–56CrossRefGoogle Scholar
  32. Roos W, Luckner M (1984) Relationships between proton extrusion and fluxes of ammonium ions and organic acid in Penicillium cyclopium. J Gen Microbiol 130:1007–1014Google Scholar
  33. Ruban V, Lopez-Sanchez JF, Pardo P, Rauret G, Muntau H, Quevauviller P (1999) Selection and evaluation of sequential extraction procedures for the determination of phosphorus forms in lake sediment. J Envirn Monit 1:51–56CrossRefGoogle Scholar
  34. Smith SE, Read DJ (1997) Mycorrhizal symbiosis, 2nd edn. Academic, New York, pp 155–159Google Scholar
  35. Subba Rao NS (ed) (1982) Advances in agricultural microbiology. Oxford and IBH Publishing, pp 229–305Google Scholar
  36. Tarafdar JC (1995) Role of VA mycorrhizal fungus on growth and water relations in wheat in presence of organic and inorganic phosphates. J Indian Soc Soil Sci 43:200–204Google Scholar
  37. Tarafdar JC, Claassen N (1988) Organic phosphorus compounds as a phosphorus source for higher plants through the activity of phosphatases produced by plant roots and microorganisms. Biol Fertil Soils 5:308–312CrossRefGoogle Scholar
  38. Tarafdar JC, Claassen N (2005) Preferential utilization of organic and inorganic sources of phosphorus by wheat plant. Plant Soil 275:285–293CrossRefGoogle Scholar
  39. Tarafdar JC, Gharu A (2006) Mobilization of organic and poorly soluble phosphates by Chaetomium globossum. Appl Soil Ecol 32:273–283CrossRefGoogle Scholar
  40. Tarafdar JC, Marschner H (1994) Phosphatase activity in the rhizospheres and hyphosphere of VA mycorrhizal wheat supplied with inorganic and organic phosphorus. Soil Biol Biochem 26:387–395CrossRefGoogle Scholar
  41. Tarafdar JC, Marschner H (1995) Dual inoculation with Aspergillus fumigates and Glomus mosseae enhances biomass production and nutrient uptake in wheat (Triticum aestivum L.) supplied with organic phosphorus as Na-phytate. Plant Soil 173:97–102CrossRefGoogle Scholar
  42. Tarafdar JC, Yadav RS (2011) Hydrolysis of P fractions by phosphatase and phytase producing fungi. Agrochimica 55(4):1–13Google Scholar
  43. Tarafdar JC, Yadav RS, Meena SC (2001) Comparative efficiency of acid phosphatase originated from plant and fungal sources. J Plant Nutr Soil Sci 164:279–282CrossRefGoogle Scholar
  44. Tisdall JM, Smith SE, Rengasamy P (1997) Aggregation of soil by fungal hyphae. Aust J Soil Res 35:55–60CrossRefGoogle Scholar
  45. Turner BL, Paphazy MJ, Haygarth PM, McKelive ID (2002) Inositol phosphates in the environment. Philos Trans R Soc Lond B 357:449–469CrossRefGoogle Scholar
  46. Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: critical adaptations by plants for securing a non renewable resource. New Phytol 157:423–447CrossRefGoogle Scholar
  47. Wyss M, Brugger R, Kronenberger A, Renny R, Fimbel R, Oesterhelt G, Lehmann M, Loon APGMY (1999) Biochemical characterization of fungal phytases (myo-inositol hexaphosphate phosphohydrolases): catalytic properties. Appl Environ Microbial 65:367–373Google Scholar
  48. Yadav RS, Tarafdar JC (2003) Phytase and phosphatase producing fungi in arid and semi-arid soils and their efficiency in hydrolyzing different organic P compounds. Soil Biol Biochem 35:745–751CrossRefGoogle Scholar
  49. Yadav BK, Tarafdar JC (2007) Ability of Emericella rugulosa to mobilize unavailable P compounds during Pearl millet (Pennisetum glaucum (L.) R. Br.) crop under arid condition. Ind J Microbiol 47:57–63CrossRefGoogle Scholar
  50. Yadav BK, Tarafdar JC (2010) Studies on phosphatases activity and clusterbean production as influenced by the P mobilization organism Emericella rugulosa. Legume Res 33:114–118Google Scholar
  51. Yadav BK, Tarafdar JC (2011) Penicillium purpurogenum, unique P mobilizers in arid-ecosystems. Arid Land Res Manage 25:87–99CrossRefGoogle Scholar
  52. Yadav BK, Tarafdar JC, panwar J, Yadav RS (2010) Phytase producing fungi and their efficiency in hydrolyzing phytin-P compounds. Ann Arid Zone 49:85–90Google Scholar
  53. Yang H, Schroeder-Moreno M, Giri B, Hu S (2018) Arbuscular mycorrhizal fungi and their responses to nutrient management. In: Giri B et al (eds) Root biology, soil biology, vol 52, pp 429–449Google Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • J. C. Tarafdar
    • 1
  1. 1.BCKVKalyaniIndia

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