Enzyme Immobilization on Chitin and Chitosan-Based Supports for Biotechnological Applications

  • Madan L. Verma
  • Sandeep Kumar
  • Anamika Das
  • Jatinder S. Randhawa
  • Munusamy Chamundeeswari
Part of the Sustainable Agriculture Reviews book series (SARV, volume 35)


Actual industrial enzymes have often high cost and instability. Such issues have restricted commercial application of such fragile biomolecules. Alternatively, immobilization of enzymes on suitable supports improves stability, cost-effectiveness and recyclability. Chitin and chitosan are ideal supporting material because they are biocompatible, biodegradable, plenty of reactive functional groups, non-toxic and cheap. Different derivatives of chitin support such as chitosan, chitosan film, chitosan nanoparticle, and chitosan nanocomposite has been used for enzyme immobilization. Chitosan-bound biomolecules display considerably improved biocatalytic potential as compared to native biomolecules. Chitosan immobilized enzymes have exceptionally high operational stability and reusability, and thus are suitable for industrial processing. This chapter reviews enzymes immobilized on chitin- and chitosan-based biomaterials, and applications to drug delivery and sustainable agriculture.


Enzymes Immobilization Stability Reusability Drug delivery Sustainable agriculture 


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Madan L. Verma
    • 1
    • 2
  • Sandeep Kumar
    • 3
  • Anamika Das
    • 4
  • Jatinder S. Randhawa
    • 5
  • Munusamy Chamundeeswari
    • 6
  1. 1.Centre for Chemistry and BiotechnologyDeakin UniversityGeelongAustralia
  2. 2.Department of BiotechnologyDr. YS Parmar University of Horticulture and ForestrySolanIndia
  3. 3.Teva API (TAPI)Greater NoidaIndia
  4. 4.University College of Paramedical SciencesGuru Kashi UniversityTalwandi SaboIndia
  5. 5.Centre for Environmental Sciences and TechnologyCentral University of PunjabBathindaIndia
  6. 6.Department of BiotechnologySt. Joseph’s College of EngineeringChennaiIndia

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