Transient Middle Cerebral Artery Occlusion Model in Rodents

  • Ying Mao
  • Wei Zhu
  • Guo-Yuan YangEmail author
Part of the Springer Series in Translational Stroke Research book series (SSTSR)


Rodent transient middle cerebral artery occlusion (MCAO) is one of the widely used focal ischemia models in the world. This model mimics human cerebral ischemic injury and provides a unique tool for the mechanistic studies of ischemia in brain tissues in vivo. Rodent ischemia and reperfusion models are commonly used because of a greater understanding of rodent genetics, the availability of specific antibodies to rodent epitopes and molecular probes in rodents, and the ability to study transgenic mouse strains. In this chapter, we discuss: (1) the preparation of MCAO in rodents; (2) the intra-luminal MCAO techniques and its modifications; (3) the quantitative evaluation of model success; and (4) the advantages and limitations of MCAO models.


Blood flow Infarct volume Middle cerebral artery occlusion Rodent Transient focal ischemia 


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Neurosurgery and Institute of Neurosurgery, Huashan HospitalFudan University School of MedicineShanghaiChina
  2. 2.Department of Anesthesia and Perioperative Care, Center for Cerebrovascular ResearchUniversity of California San FranciscoSan FranciscoUSA
  3. 3.Department of Neurological SurgeryUniversity of California San FranciscoSan FranciscoUSA

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