Experimental Laceration Spinal Cord Injury Model in Rodents
Experimental laceration spinal cord injury (l-SCI) is an important in vivo model to investigate mechanisms of axonal regeneration and neurite plasticity following SCI. l-SCI is customarily performed freehand under visual guidance that results in variable lesion depths and shapes. In this chapter, a precise l- SCI model will be described for rodents using the Louisville Injury System Apparatus (LISA). This method incorporates: (1) reliable vertebral stabilization and spinal cord exposure and (2) accurate tissue laceration without contusion.
KeywordsSpinal cord injury Laceration SCI model Louisville Injury System Apparatus (LISA) Axonal regeneration
We acknowledge Norton Healthcare for their continued support.
- 1.Bunge RP, Puckett WR, Becerra JL, Marcillo A, Quencer RM. Observations on the pathology of human spinal cord injury. A review and classification of 22 new cases with details from a case of chronic cord compression with extensive focal demyelination. Adv Neurol. 1993;59:75–89.PubMedPubMedCentralGoogle Scholar
- 17.Simonen M, Pedersen V, Weinmann O, Schnell L, Buss A, Ledermann B, Christ F, Sansig G, van der Putten H, Schwab ME. Systemic deletion of the myelin-associated outgrowth inhibitor Nogo-A improves regenerative and plastic responses after spinal cord injury. Neuron. 2003;38:201–11.CrossRefGoogle Scholar
- 23.Zhang YP, Iannotti C, Shields LBE, Han Y, Burke DA, Xu XM, Shields CB. Dural closure, cord approximation, and clot removal: enhancement of tissue sparing in a novel laceration spinal cord injury model. J Neurosurg. 2004;100:343–52.Google Scholar
- 25.Zhang YP, Walker MJ, Shields LBE, Wang X, Walker CL, Xu XM, Shields CB. Controlled cervical laceration injury in mice. J Vis Exp. 2013:50030.Google Scholar