Advertisement

Immobilization Methods of Enzymes: Part I

  • Kelvii Wei Guo
Chapter
Part of the Fungal Biology book series (FUNGBIO)

Abstract

The crucial issue of cathode construction is to permanently immobilize enzymes on cathode with the activity of enzymes retaining. The achievement of the immobilization of enzymes can be realized by physical and chemical methods, where physical methods include adsorption and gel entrapment. Although simple and no additional reagents are required, the physical association between enzymes and electrode surface is weak. However, chemical methods involve covalent bonding between enzyme and support material. Interaction between enzyme and support is strong, but concern grows over the fact that covalent bonding can disrupt enzyme activity. Enzyme immobilization has also been used to provide electrochemical communication between electrode and enzyme active center.

Consequently, the immobilization methods of enzymes are marked for showing the crucial information to take advantage of the captivating properties of the immobilized enzymes.

Keywords

Enzyme Immobilization methods Physical method Adsorption Gel entrapment Chemical method Covalent bonding Cross-linking 

References

  1. Alamsyah G, Albels VA, Sahlan M, Hermansyah H (2017) Effect of chitosan’s amino group in adsorption-crosslinking immobilization of lipase enzyme on resin to catalyze biodiesel synthesis. Energy Procedia 136:47–52CrossRefGoogle Scholar
  2. Barbosa O, Ortiz C, Berenguer-Murcia Á, Torres R, Rodrigues RC, Fernandez-Lafuente R (2015) Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts. Biotechnol Adv 33(5):435–456CrossRefGoogle Scholar
  3. Bernal C, Rodríguez K, Martínez R (2018) Integrating enzyme immobilization and protein engineering: an alternative path for the development of novel and improved industrial biocatalysts. Biotechnol Adv 36(5):1470–1480CrossRefGoogle Scholar
  4. Cracknell JA, Vincent KA, Armstrong FA (2008) Enzymes as working or inspirational electrocatalysts for fuel cells and electrolysis. Chem Rev 108:2439–2461CrossRefGoogle Scholar
  5. Das R, Talat M, Srivastava ON, Kayastha AM (2018) Covalent immobilization of peanut β-amylase for producing industrial nanobiocatalysts: a comparative study of kinetics, stability and reusability of the immobilized enzyme. Food Chem 245:488–499CrossRefGoogle Scholar
  6. Grigoras AG (2017) Catalase immobilization-A review. Biochem Eng J Part B 117:1–20CrossRefGoogle Scholar
  7. Karajanagi SS, Vertegel AA, Kane RS, Dordick JS (2004) Structure and function of enzymes adsorbed onto single-walled carbon nanotubes. Langmuir 20:11594–11599CrossRefGoogle Scholar
  8. Khaldi K, Sam S, Lounas A, Yaddaden C, Gabouze NE (2017) Comparative investigation of two methods for Acetylcholinesterase enzyme immobilization on modified porous silicon. Appl Surf Sci 421:148–154CrossRefGoogle Scholar
  9. Mehta J, Bhardwaj N, Bhardwaj SK, Kim KH, Deep A (2016) Recent advances in enzyme immobilization techniques: metal-organic frameworks as novel substrates. Coord Chem Rev 322:30–40CrossRefGoogle Scholar
  10. Müller F, Torger B, Allertz PJ, Jähnichen K, Keßler S, Müller M, Simon F, Salchert K, Mäurer H, Pospiech D (2018) Multifunctional crosslinkable itaconic acid copolymers for enzyme immobilization. Eur Polym J 102:47–55CrossRefGoogle Scholar
  11. Nishida VS, de Oliveira RF, Brugnari T, Correa RCG, Peralta RA, Castoldi R, de Souza CGM, Bracht A, Peralta RM (2018) Immobilization of Aspergillus awamori β-glucosidase on commercial gelatin: an inexpensive and efficient process. Int J Biol Macromol 111:1206–1213CrossRefGoogle Scholar
  12. Sharifi M, Robatjazi SM, Sadri M, Mosaabadi JM (2018) Covalent immobilization of organophosphorus hydrolase enzyme on chemically modified cellulose microfibers: statistical optimization and characterization. React Funct Polym 124:162–170CrossRefGoogle Scholar
  13. Shen L, Cheng KCK, Schroeder M, Yang P, Marsh ENG, Lahann J, Chen Z (2016) Immobilization of enzyme on a polymer surface. Surf Sci 648:53–59CrossRefGoogle Scholar
  14. Shinde P, Musameh M, Gao Y, Robinson AJ, Kyratzis I (2018) Immobilization and stabilization of alcohol dehydrogenase on polyvinyl alcohol fibre. Biotechnol Rep 19:e00260CrossRefGoogle Scholar
  15. Souza CJF, Garcia-Rojas EE, Favaro-Trindade CS (2018) Lactase (β-galactosidase) immobilization by complex formation: impact of biopolymers on enzyme activity. Food Hydrocoll 83:88–96CrossRefGoogle Scholar
  16. Tang C, Saquing CD, Sarin PK, Kelly RM, Khan SA (2014) Nanofibrous membranes for single-step immobilization of hyperthermophilic enzymes. J Membr Sci 472:251–260CrossRefGoogle Scholar
  17. Torres JA, Silva MC, Lopes JH, Nogueira AE, Nogueira FGE, Corrêa AD (2018) Development of a reusable and sustainable biocatalyst by immobilization of soybean peroxidase onto magnetic adsorbent. Int J Biol Macromol 114:1279–1287CrossRefGoogle Scholar
  18. Ulu A, Ozcan I, Koytepe S, Ates B (2018) Design of epoxy-functionalized Fe3O4@MCM-41 core–shell nanoparticles for enzyme immobilization. Int J Biol Macromol 115:1122–1130CrossRefGoogle Scholar
  19. Vaz RP, de Souza Moreira LR, Filho EXF (2016) An overview of holocellulose-degrading enzyme immobilization for use in bioethanol production. J Mol Catal B Enzym 133:127–135CrossRefGoogle Scholar
  20. Voběrková S, Solčány V, Vršanská M, Adam V (2018) Immobilization of ligninolytic enzymes from white-rot fungi in crosslinked aggregates. Chemosphere 202:694–707CrossRefGoogle Scholar
  21. Wong DE, Senecal KJ, Goddard JM (2017) Immobilization of chymotrypsin on hierarchical nylon 6,6 nanofiber improves enzyme performance. Colloids Surf B Biointerfaces 154:270–278CrossRefGoogle Scholar
  22. Wu JCY, Hutchings CH, Lindsay MJ, Werner CJ, Bundy BC (2015) Enhanced enzyme stability through site-directed covalent immobilization. J Biotechnol 193:83–90CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Kelvii Wei Guo
    • 1
  1. 1.Department of Mechanical and Biomedical EngineeringCity University of Hong KongKowloon Tong, KowloonHong Kong

Personalised recommendations