Immobilization Methods of Enzymes: Part I
The crucial issue of cathode construction is to permanently immobilize enzymes on cathode with the activity of enzymes retaining. The achievement of the immobilization of enzymes can be realized by physical and chemical methods, where physical methods include adsorption and gel entrapment. Although simple and no additional reagents are required, the physical association between enzymes and electrode surface is weak. However, chemical methods involve covalent bonding between enzyme and support material. Interaction between enzyme and support is strong, but concern grows over the fact that covalent bonding can disrupt enzyme activity. Enzyme immobilization has also been used to provide electrochemical communication between electrode and enzyme active center.
Consequently, the immobilization methods of enzymes are marked for showing the crucial information to take advantage of the captivating properties of the immobilized enzymes.