Comparative Study of Cellulase Production Using Submerged and Solid-State Fermentation

  • Prabhakara Rao Dasari
  • Pramod W. Ramteke
  • Satyam Kesri
  • Prasada Rao Kongala
Part of the Fungal Biology book series (FUNGBIO)


There’s an expanding demand for cellulases for different applications, among which the bioconversion of submerged and solid-state fermentation for cellulose production is the major one. Enhancements within the titers, as well as particular exercises of cellulases, are exceedingly wanted for its utility in bioethanol generation and other applications. This audit bargains with improvements in bioprocess innovations, solid-state and submerged maturation, as well as on the techniques embraced for progressing cellulase generation properties, counting designing the qualities. It moreover gives a brief diagram of commercially accessible cellulase arrangements. Submerged and solid-state fermentation have been widely used for the production of cellulose in a wide variety of substances that are highly beneficial to individuals and industry. Over a long time, maturation strategies have picked up monstrous significance due to their financial and natural preference development of new machinery and processes. Two broad fermentation techniques have emerged as a result of this rapid development: submerged fermentation (SmF) and solid-state fermentation (SSF). These methods have been in advance altered and refined to maximize efficiency. Discovery of the beneficial activity of several secondary metabolites produced by microorganisms (bioactive compounds) has resulted in the further exploration of fermentation as a production technique for these compounds. At the research level, both SSF and SmF have been used; however, some techniques yielded better results than others. Much work still needs to be done to identify the best fermentation technique for each bioactive compound. This paper presents the different fermentation techniques for the production of cellulose compound. This paper presents comparative analysis of submerge and solid state fermentations for production of cellulase.


Cellulase enzyme Enzyme production Solid state fermentation Lignocellulosics biomass Biofuels 


  1. Acharya PB, Acharya DK, Modi HA (2008) Optimization for cellulase production by Aspergillus Niger using saw dust as substrate. Afr J Biotechnol 7:4147–4152Google Scholar
  2. Acharya BK, Mohana S, Jog R, Divecha J, Madamwar D (2010) Utilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation. J Environ Manag 91:2019–2027CrossRefGoogle Scholar
  3. Agrawal R, Satlewal A, Verma AK (2013) Production of an extracellular cellobiase in solid state fermentation. J Microbiol Biotechnol Food Sci 2:2339–2350Google Scholar
  4. Ahuja SK, Ferreira GM, Moreira AR (2004) Utilization of enzymes for environmental applications. Crit Rev Biotechnol 24(2–3):125–154PubMedCrossRefGoogle Scholar
  5. Ang SK, Shaza EM, Adibah Y, Suraini AA, Madihah MS (2013) Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation. Process Biochem 48:1293–1302CrossRefGoogle Scholar
  6. Assamoi AA, Jacqueline D, Delvigne F, Lognay G, Thonart P (2008) Solid-state fermentation of xylanase from Penicillium canescens 10-10c in a multi-layer-packed bed reactor. Appl Biochem Biotechnol 145:87–97PubMedCrossRefPubMedCentralGoogle Scholar
  7. Babu KR, Satyanarayana T (1996) Production of bacterial enzymes by solid state fermentation. J Sci Ind Res 55:464–467Google Scholar
  8. Basha NS, Rekha R, Komala M, Ruby S (2009) Production of extracellular anti-leukaemic enzyme lasparaginase from marine actinomycetes by solid state and submerged fermentation: purification and characterisation. Trop J Pharm Res 8(4):353–360CrossRefGoogle Scholar
  9. Bhat MK (2000) Cellulases and related enzymes in biotechnology. Biotechnol Adv 18(5):355–383PubMedPubMedCentralCrossRefGoogle Scholar
  10. Biswas SR, Jana SC, Mishra AK, Nanda G (1990) Production, purification and characterization of xylanase from a hyperxylanotic mutant of Aspergillus ochraceus. Biotechnol Bioeng 35:244–251PubMedCrossRefGoogle Scholar
  11. Brijwani K, Oberoi HS, Vadlani PV (2010) Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran. Process Biochem 45(1):120–128CrossRefGoogle Scholar
  12. Cen P, Xia L (1999) Production of cellulase in solid state fermentation. In: Scheper T (ed) Recent progress in bioconversion of lignocellulosics, Advances in biochemical engineering/biotechnology, vol 65. Springer, Berlin, p 69CrossRefGoogle Scholar
  13. Coradi GV, Da Visitação VL, De Lima EA, Saito LYT, Palmieri DA, Takita MA et al (2013) Comparing submerged and solid-state fermentation of agro-industrial residues for the production and characterization of lipase by Trichoderma harzianum. Ann Microbiol 63:533–540CrossRefGoogle Scholar
  14. Coughlan MP (1985) Cellulase production, properties and applications. Biochem Soc Trans 13:405–406PubMedCrossRefGoogle Scholar
  15. Cunha FM, Esperanca MN, Zangirolami TC, Badino AC, Farinas CS (2012) Sequential solid-state and submerged cultivation of Aspergillus Niger on sugarcane bagasse for the production of cellulase. Bioresour Technol 112:270–274CrossRefGoogle Scholar
  16. Da Silva DP, Pirota RDB, Codima CA, Tremacoldi CR, Rodrigues A, Farinas CS (2012) Using Amazon forest fungi and agricultural residues as a strategy to produce cellulolytic enzymes. Biomass Bioenergy 37:243–250CrossRefGoogle Scholar
  17. Datta M, Patel S, Parikh H (1989) Solid state fermentation for cellulases and β-glucosidase production by Aspergillus Niger. J Ferment Bioeng 67:424–426CrossRefGoogle Scholar
  18. Debing J, Peijun L, Stagnitti F, Xianzhe X, Li L (2006) Pectinase production by solid fermentation from Aspergillus Niger by a new prescription experiment. Ecotox Environ Safe 64:244–250CrossRefGoogle Scholar
  19. Demain AL (1999) Pharmaceutically active secondary metabolites of microorganisms. Appl Microbiol Biotechnol 52:455–463PubMedCrossRefGoogle Scholar
  20. Du R, Su R, Li X, Tantai X, Liu Z, Yang J (2012) Controlled adsorption of cellulase onto pretreated corncob by pH adjustment. Cellulose 19:371–380CrossRefGoogle Scholar
  21. Durand A, Chereau D (1988) A new pilot reactor for solid-state fermentation: application to the protein enrichment of sugar beet pulp. Biotechnol Bioeng 31(5):476–486PubMedCrossRefGoogle Scholar
  22. Durand R, Renaud J, Maratray SA, Diez M (1996) INRA-Dijon reactors for solid state fermentation: designs and applications. J Sci Ind Res 55(5–6):317–332Google Scholar
  23. Ekundayo FO, Ekundayo EA, Ayodele BB (2017) Comparative studies on glucanases and beta-glucosidase activities of Pleurotus ostreatus and P. Pulmonarius in solid state fermentation. Mycosphere 8(8):1201–1209CrossRefGoogle Scholar
  24. Emert GH, Katzen R (1980) Gulf’s cellulose-to-ethanol process. CHEMTECH 10(10):610–615Google Scholar
  25. Enari TM (1983) Microbial cellulases. In: Forgaty WF (ed) Microbial enzymes and biotechnology, vol I. Applied Sciences Publishers, London, pp 83–223Google Scholar
  26. Fadel M (2000) Production physiology of cellulases and β-glucosidase enzymes of Aspergillus niger grown under solid state fermentation conditions. Online Biol Sci 1:401–411Google Scholar
  27. Farinas CS (2018) Solid-state fermentation for the on-site production of cellulolytic enzymes and their use in the saccharification of lignocellulosic biomass. In: Current developments in biotechnology and bioengineering. Elsevier, Amsterdam, pp 169–183CrossRefGoogle Scholar
  28. Florencio C, Cunha FM, Badino AC, Farinas CS (2015) Validation of a novel sequential cultivation method for the production of enzymatic cocktails from Trichoderma strains. Appl Biochem Biotechnol 175:1389–1402PubMedCrossRefGoogle Scholar
  29. Gautam SP, Bundela PS, Pandey AK, Awasthi MK, Sarsaiya S (2010) Optimization of the medium for the production of cellulase by the Trichoderma viride using submerged fermentation. Int J Environ Sci 1(4):656Google Scholar
  30. Ghildyal NP, Lonsane BK, Sreekantiah KR, Sreenivasa Murthy V (1985) Economics of submerged and solid state fermentation for the production of amyloglucosidase. J Food SciTechnol 22:171–176Google Scholar
  31. Goyal M, Kalra KL, Sareen VK, Soni G (2008) Xylanase production with xylan rich lignocelulasic waste by a local soil isolate of Trichoderma viride. Braz J Microbiol 39:535–541PubMedPubMedCentralCrossRefGoogle Scholar
  32. Grajek W (1987) Comparative studies on the production of cellulases by thermophilic fungi in submerged and solid-state fermentation. Appl Microbiol Biotechnol 26(2):126–129CrossRefGoogle Scholar
  33. Graminha EB, Gonçalves AZ, Pirota RD, Balsalobre MA, Da Silva R, Gomes E (2008) Enzyme production by solid-state fermentation: application to animal nutrition. Anim Feed Sci Technol 144(1–2):1–22CrossRefGoogle Scholar
  34. Gupte A, Madamwar D (1997) Solid state fermentation of lignocellulosic waste for cellulase and β-glucosidase production by cocultivation of Aspergillus ellipticus and Aspergillus fumigatus. Biotechnol Prog 13(2):166–169CrossRefGoogle Scholar
  35. Gutierrez-Correa M, Portal L, Moreno P, Tengerdy RP (1999) Mixed culture solid substrate fermentation of Trichoderma reesei with Aspergillus Niger on sugar cane bagasse. Bioresour Technol 68(2):173–178CrossRefGoogle Scholar
  36. Hafiz, Muhammad Nasir I, Ishtiaq A, Muhammad Anjum Z, Muhammad I (2011) Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility. Adv Biosci Biotechnol 2(3):149–156Google Scholar
  37. Hesseltine CW (1972) Solid state fermentations. Biotechnol Bioeng 14(5):17–32Google Scholar
  38. Hesseltine CW (1977) Solid state fermentation-part 1. Process Biochem 12:24–27Google Scholar
  39. Hesseltine CW (1987) Solid state fermentation—an overview. Int Biodeterior 23(2):79–89CrossRefGoogle Scholar
  40. Hong J, Tamaki H, Akiba S et al (2001) Cloning of a gene encoding a highly stable endo-b-1,4-glucanase from Aspergillus niger and its expression in yeast. J Biosci Bioeng 92:434–441PubMedCrossRefGoogle Scholar
  41. Hui L, Wan C, Hai-tao D, Xue-jiao C, Qi-fa Z, Yu-hua Z (2010) Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation. Bioresour Technol 101:7556–7562CrossRefGoogle Scholar
  42. Isaac GS, Abu-Tahon MA (2015) Enhanced alkaline cellulases production by the thermohalophilic Aspergillus terreus AUMC 10138 mutated by physical and chemical mutagens using corn stover as substrate. Braz J Microbiol 46(4):1269–1277PubMedPubMedCentralCrossRefGoogle Scholar
  43. Ishida N, Saitoh S, Ohnishi T, Tokuhiro K et al (2006) Metabolic engineering of Saccharomyces cerevisiae for efficient production of pure L-(+)-lactic acid. Appl Biochem Biotechnol 131:795–807PubMedCrossRefGoogle Scholar
  44. Iqbal, Hafiz Muhammad Nasir, et al. “Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility.” Advances in Bioscience and Biotechnology 2.03 (2011): 149–156.CrossRefGoogle Scholar
  45. Jabasingh SA, Nachiyar CV (2011) Utilization of pretreated bagasse for the sustainable bioproduction of cellulase by Aspergillus nidulans MTCC344 using response surface methodology. Ind Crop Prod 34:1564–1571CrossRefGoogle Scholar
  46. Jalak J, Kurasin M, Teugjas H, Valjama P (2012) Endo-exo synergism in cellulose hydrolysis revisited. J Biol Chem 287(34):28802–28815PubMedPubMedCentralCrossRefGoogle Scholar
  47. Karmakar M, Ray R (2011) Current trends in research and application of microbial cellulases. Res J Microbiol 6:41–53CrossRefGoogle Scholar
  48. Kirk O, Borchert TV, Fuglsang CC (2002) Industrial enzyme applications. Curr Opin Biotechnol 13(4):345–351PubMedCrossRefGoogle Scholar
  49. Klein-Marcuschamer D, Oleskowicz-Popiel P, Simmons BA, Blanch HW (2012) The challenge of enzyme cost in the production of lignocellulosic biofuels. Biotechnol Bioeng 109(4):1083–1087PubMedCrossRefPubMedCentralGoogle Scholar
  50. Kotwal SM, Gote MM, Sainkar SR, Khan MI, Khire JM (1998) Production of α-galactosidase by thermophilic fungus Humicola sp. in solid-state fermentation and its application in soyamilk hydrolysis. Process Biochem 33(3):337–343CrossRefGoogle Scholar
  51. Kuhad RC, Mehta G, Gupta R, Sharma KK (2010) Fed batch enzymatic saccharification of newspaper cellulosics improves the sugar content in the hydrolysates and eventually the ethanol fermentation by Saccharomyces cerevisiae. Biomass Bioenergy 7:1–6Google Scholar
  52. Kumar S, Sharma HK, Sarkar BC (2011) Effect of substrate and fermentation conditions on pectinase and cellulase production by Aspergillus Niger NCIM 548 in submerged (SmF) and solid state fermentation (SSF). Food Sci Biotechnol 20(5):1289CrossRefGoogle Scholar
  53. Lawal SA, Ugheoke BI (2010) Investigation of alpha-cellulose content of agro-waste products as alternatives for paper production. AU J Technol 13:258–260Google Scholar
  54. Lee YB, Lee B, Jo K, Lee N, Chang C, Lee Y, Lee J (2007) Purification and characterization of cellulase by Bacillus amyoliquefaciens DL3, utilizing rice hull. Bioresour Technol 98(2):288–297CrossRefGoogle Scholar
  55. Lee CK, Darah I, Ibrahim CO (2011) Production and optimization of cellulase enzyme using Aspergillus niger USM AI 1 and comparison with Trichoderma reesei via solid state fermentation system. Biotechnol Res Int 2011:1CrossRefGoogle Scholar
  56. Lever M, Ho G, Cord-Ruwisch R (2010) Ethanol from lignocellulose using crude unprocessed cellulose from solid-state fermentation. Bioresour Technol 101(18):7083–7087CrossRefGoogle Scholar
  57. Limayem A, Ricke SC (2012) Lignocellulosic biomass for bioethanol production: current perspectives, potential issues and future prospects. Prog Energy Combust Sci 38:449–467CrossRefGoogle Scholar
  58. Liu BL, Tzeng YM (1998) Optimization of growth medium for the production of spores from Bacillus thuringiensis using response surface methodology. Bioprocess Biosyst Eng 18:413–418Google Scholar
  59. Liu J, Xue D, He K, Yao S (2012) Cellulase production in solid-state fermentation by marine Aspergillus sp. ZJUBE-1 and its enzymological properties. Adv Sci Lett 16(1):381–386CrossRefGoogle Scholar
  60. Lonsane BK, Ramakrishna M (1989) Microbial enzymes in food processing industry: present status and future prospects in India. Indian Food Industry 8(4):15–31Google Scholar
  61. Ming C, Jing Z, Liming X (2008) Enzymatic hydrolysis of maize straw polysaccharides for the production of reducing sugars. Carbohydr Polym 71(3):411–415CrossRefGoogle Scholar
  62. Mitchell DA, Berovic M, Krieger N (2006) Introduction to solid state fermentation bioreactors. In: Mitchell DA, Krieger N, Berovi M (eds) Solid state bioreactors: fundamentals of design and operation. Springer, Berlin, pp 33–44CrossRefGoogle Scholar
  63. Moo-Young M, Moriera AR, Tengerdy RP (1983) Principles of solid state fermentation. In: Smith JE, Berry DR, Kristiansen B (eds) The filamentous fungi, Fungal biotechnology, vol 4. Edward Arnold Publishers, London, pp 117–144Google Scholar
  64. Mrudula S, Murugammal R (2011) Production of cellulase by Aspergillus Niger under submerged and solid state fermentation using coir waste as a substrate. Braz J Microbiol 42(3):1119–1127PubMedPubMedCentralCrossRefGoogle Scholar
  65. Nampoothiri KM, Pandey A (1996) Solid-state fermentation for L-glutamic acid production using Brevibacterium sp. Biotechnol Letts 18(2):199–204CrossRefGoogle Scholar
  66. Neagu DA, DESTAIN J, THONART P, SOCACIU C (2012) Trichoderma reesei cellulase produced by submerged versus solid state fermentations. Bull UASVM Agric 69:2Google Scholar
  67. Nema N, Alamir L, Mohammad M (2015) Production of cellulase from Bacillus cereus by submerged fermentation using corn husks as substrates. Int Food Res J 22(5):1831–1836.Google Scholar
  68. Padmavathi T, Nandy V, Agarwal P (2012) Optimization of the medium for the production of cellulases by Aspergillus terreus and Mucor plumbeus. Eur J Exp Biol 2(4):1161–1170Google Scholar
  69. Palaniyappan M, Vijayagopa V, Viswanathan R, Viruthagiri T (2009) Screening of natural substrates and optimization of operating variables on the production of pectinase by submerged fermentation using Aspergillus niger MTCC 281. Afr J Biotechnol 8:682–686Google Scholar
  70. Pandey A (1991) Aspects of fermenter design for solid-state fermentations. Process Biochem 26(6):355–361CrossRefGoogle Scholar
  71. Pandey A (1992) Recent development in solid state fermentation. Process Biochem 27(2):109–117CrossRefGoogle Scholar
  72. Pandey A (1996) Recent developments in solid-state fermentation. Process Biochem 27:109–117CrossRefGoogle Scholar
  73. Pandey A (2003) Sold-state fermentation. Biochem Eng J 13:81–84CrossRefGoogle Scholar
  74. Pandey A, Selvakumar P, Soccol CR, Nigam P (1999) Solid-state fermentation for the production of industrial enzymes. Curr Sci 77:149–162Google Scholar
  75. Pandey A, Soccol CR, Nigam P, Soccol VT (2000) Biotechnological potential of agro- industrial residues: sugarcane bagasse. Bioresour Technol 74(1):69–80CrossRefGoogle Scholar
  76. Patil SR, Dayanand A (2006) Exploration of regional agrowastes for the production of pectinase by Aspergillus niger. Food Technol Biotech 44:289–292Google Scholar
  77. Pirota RD, Delabona PS, Farinas CS (2014) Simplification of the biomass to ethanol conversion process by using the whole medium of filamentous fungi cultivated under solid-state fermentation. Bioenergy Res 7:744–752CrossRefGoogle Scholar
  78. Ragauskas AJ, Williams CK, Davison BH, Britovsek G, Cairney J, Eckert CA, Mielenz JR (2006) The path forward for biofuels and biomaterials. Science 311(5760):484–489CrossRefGoogle Scholar
  79. Raimbault M (1988) Enzymes production by solid state fermentation. In: Solid state fermentation in bioconversion of agro-industrial raw materials, vol 5. ORSTOM Centre Montpellier, MontpellierGoogle Scholar
  80. Raimbault M (1998) General and microbiological aspects of solid substrate fermentation. Electron J Biotechnol 27:498–503Google Scholar
  81. Ray RC (2011) Solid-state fermentation for production of microbial cellulase: an overview. In: Cellulase: types and action, mechanism, and uses. Nova Science Publishers Inc, New YorkGoogle Scholar
  82. Reddy GPK, Narasimha G, Kumar KD, Ramanjaneyulu G, Ramya A, Kumari BS, Reddy BR (2015) Cellulase production by Aspergillus Niger on different natural lignocellulosic substrates. Int J Curr Microbiol App Sci 4(4):835–845Google Scholar
  83. Robinson T, Nigam P (2003) Bioreactor design for protein enrichment of agricultural residues by solid state fermentation. Biochem Eng J 13:197–203CrossRefGoogle Scholar
  84. Robinson T, Singh D, Nigam P (2001) Solid-state fermentation: a promising microbial technology for secondary metabolite production. Appl Microbiol Biotechnol 55:284–289PubMedCrossRefGoogle Scholar
  85. Romero-Gómez SJ, Augur C, Viniegra-González G (2000) Invertase production by Aspergillus Niger in submerged and solid-state fermentation. Biotechnol Lett 22(15):1255–1258CrossRefGoogle Scholar
  86. Roussos S (1989) Sugarcane used in solid state fermentation for cellulases production. Solid State Ferment:139–150Google Scholar
  87. Roussos S, Hannibal L, Aquiahuatl MA, Trejo M, Marakatis S (1994) Caffeine degradation by Penicillium verrucosum in solid fermentation of coffee pulp: critical effect of additional inorganic and organic nitrogen sources. J Food Sci Technol 31(4):316–319Google Scholar
  88. Ruth MF, Howard JA, Nikolov Z, Hooker BS et al (1999) Preliminary economic analysis of agricultural production for use in cellulose hydrolysis. In: Abstracts from the 21st symposium on biotechnology for fuels and chemicals, Fort Collins, Colorado, Mays, Abstract, 3–49Google Scholar
  89. Sadhu S, Maiti TK (2013) Cellulase production by bacteria: a review. Brit Microbial Res J 3(3):235–258CrossRefGoogle Scholar
  90. Sekar C, Balaraman K (1998) Optimization studies on the production of cyclosporin A by solid state fermentation. Bioprocess Eng 18(4):293–296CrossRefGoogle Scholar
  91. Shobana P, Maheswari NU (2013) Production of cellulase from Aspergillus fumigatus under submerged and solid state fermentation using agricultural waste. Int J Adv Pharm Biol Chem 2(4):595–599Google Scholar
  92. Shweta A (2015) Solid state fermentation for cellulase production. Biotechnol Res 1(1):108–112Google Scholar
  93. Singh A, Singh N, Bishnoi NR (2009) Production of cellulases by Aspergillus heteromorphus from wheat straw under submerged fermentation. Int J Environ Sci Eng 1:1Google Scholar
  94. Singhania RR, Sukumaran RK, Patel AK, Larroche C, Pandey A (2010) Advancement and comparative profiles in the production technologies using solid-state and submerged fermentation for microbial cellulases. Enzym Microb Technol 46(7):541–549CrossRefGoogle Scholar
  95. Souza PM, Magalhaes PO (2010) Application of microbial - amylase in industry-a review. Braz J Microbiol 41:850–861PubMedPubMedCentralCrossRefGoogle Scholar
  96. Stockton BC, Mitchell DJ, Grohmann K, Himmel ME (1991) Optimum b-D glucosidase supplementation of cellulase for efficient conversion of cellulose to glucose. Biotechnol Lett 13:57–62CrossRefGoogle Scholar
  97. Subramaniyam R, Vimala R (2012) Solid state and submerged fermentation for the production of bioactive substances: a comparative study. Int J Sci Nat 3(3):480–486Google Scholar
  98. Sukumaran RK, Singhania RR, Mathew GM, Pandey A (2009) Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bioethanol production. Renew Energy 34(2):421–424CrossRefGoogle Scholar
  99. Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83(1):1–11PubMedCrossRefGoogle Scholar
  100. Szendefy J, Szakacs G, Christopher L (2006) Potential of solid-state fermentation enzymes of Aspergillus oryzae in biobleaching of paper pulp. Enzym Microb Tech 39:1354–1360CrossRefGoogle Scholar
  101. Vandenberghe LP, Soccol CR, Pandey A, Lebeault JM (2000) Solid-state fermentation for the synthesis of citric acid by Aspergillus Niger. Bioresour Technol 74(2):175–178CrossRefGoogle Scholar
  102. Vintila T, Dragomirescu M, Jurcoane S, Vintila D, Caprita R, Maniu M (2009) Production of cellulase by submerged and solid-state cultures and yeasts selection for conversion of lignocellulose to ethanol. Rom Biotechnol Lett 14(2):4275–4281Google Scholar
  103. Wen Z, Liao W, Chen S (2005) Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure. Process Biochem 40:3087–3094CrossRefGoogle Scholar
  104. Xue M, Liu D, Zhang H, Qi H, Lei Z (1992) A pilot process of solid state fermentation from sugar beet pulp for the production of microbial protein. J Ferment Bioeng 73(3):203–205CrossRefGoogle Scholar
  105. Yang SQ, Yan QJ, Jiang ZQ et al (2006) High-level of xylanase production by the thermophilic Paecilomyces thermophila J18 on wheat straw in solid-state fermentation. Bioresour Technol 97:1794–1800PubMedCrossRefGoogle Scholar
  106. Zeng W, Chen HZ (2009) Air pressure pulsation solid state fermentation of feruloyl esterase by Aspergillus niger. Bioresour Technol 100:1371–1375PubMedCrossRefGoogle Scholar
  107. Zhiyou W, Liao W, Shulin C (2004) Hydrolysis of animal manure lignocellulosics for reducing sugar production. Bioresour Technol 91(1):31–39CrossRefGoogle Scholar

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Authors and Affiliations

  • Prabhakara Rao Dasari
    • 1
  • Pramod W. Ramteke
    • 2
  • Satyam Kesri
    • 1
  • Prasada Rao Kongala
    • 1
  1. 1.Department of Chemistry, Sam Higginbottom University of Agriculture Technology and Sciences, Allahabad, Department of Biological Sciences, Sam Higginbottom University of Agriculture Technology & SciencesPrayagrajIndia
  2. 2.Department of Biological SciencesSam Higginbottom University of Agriculture, Technology and Sciences (SHUATS)AllahabadIndia

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