Potential of Microalgae for Integrated Biomass Production Utilizing CO2 and Food Industry Wastewater

  • Jitendra Mehar
  • Ajam Shekh
  • Nethravathy Uthaiah Malchira
  • Sandeep Mudliar


The concomitant generation of renewable energy and material resources with distinct environmental applications for CO2 mitigation and wastewater treatment is one of the hallmarks of microalgal research. Microalgae are photoautotrophic microorganisms with simple growth requirements (light, CO2, N, P, and K) that can synthesize commodity biomolecules (lipids, proteins, and carbohydrates) and high-value metabolites in large amount over a short period of time. Requirement of microalgae for C, N, P, and K is usually met by providing technical grade chemicals which ultimately increases the cost of biomass production. However, since microalgae have the potential to utilize CO2 as well as N, P, and K from wastewater, high-density cultivation of microalgae can be accomplished by utilizing wastewater and CO2.

Microalgae biomass produced through CO2 fixation and wastewater treatment can potentially be used for the production of biofuels, pharmaceuticals, and feed grade products. The use of wastewater with co-utilization of CO2 for microalgae cultivation is beneficial since it reduces the requirements of freshwater and essential nutrients (N, P, and K). Wastewater generated from domestic, agricultural, and industrial activities contains a variety of ingredients which can be utilized as a cultivation medium for microalgae. Cultivation of microalgae using wastewater also helps in removal of COD, nitrates, and phosphates aiding its safe disposal and/or utilization. This chapter summarizes the potential of microalgae for integrated biomass production utilizing CO2 and food industry wastewater. The authors focus on the concepts and application of CO2 and wastewater utilization by microalgae. The challenges and future needs for cultivation of microalgae in wastewater are also reviewed.


CO2 sequestration  Integrated biomass production  Wastewater treatment  Food industry wastewater 


  1. Adenan NS, Yusoff FM, Medipally SR, Shariff M (2016) Enhancement of lipid production in two marine microalgae under different levels of nitrogen and phosphorus deficiency. J Environ Biol 37:669–679Google Scholar
  2. Azadia P, Brownbridgea PEA, Mosbacha S, Inderwildib OR, Kraft M (2014) Production of biorenewable hydrogen and syngas via algae gasification: a sensitivity analysis. Energy Procedia 61:2767–2770CrossRefGoogle Scholar
  3. Aziz MA, Ng WJ (1992) Feasibility of wastewater treatment using the activated-algae process. Water Sci Technol 28:71–76CrossRefGoogle Scholar
  4. Badger MR, Price GD (1994) The role of carbonic anhydrase in photosynthesis. Annu Rev Plant Physiol Plant Mol Bio 45:369–392CrossRefGoogle Scholar
  5. Balannec BM, Vourch M, Rabiller-Baudry, Chaufer B (2005) Comparative study of different nanofiltration and reverse osmosis membranes for dairy effluent treatment by dead-end filtration. Sep Purif Technol 42:195–200CrossRefGoogle Scholar
  6. Barsanti L, Gualtieri P (2006) Algae-anatomy, biochemistry and biotechnology, 2nd edn. CRC Press, USA, pp 162–209Google Scholar
  7. Becker EW (1988) Micro-algae for human and animal consumption. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge, pp 222–256Google Scholar
  8. Becker EW (1994) Microalgae: biotechnology and microbiology. Cambridge University Press, Cambridge. ISBN 978-0-521-06113Google Scholar
  9. Becker EW (2004) Microalgae in human and animal nutrition. In: Richmond A (ed) Handbook of microalgal culture. Biotechnology and applied phycology. Blackwell Science, Oxford, pp 312–351Google Scholar
  10. Beneroso D, Bermudez JM, Arenillas A, Menendez JA (2014) Microwave pyrolysis of microalgae for high syngas production. Bioresour Technol 144:240–246CrossRefGoogle Scholar
  11. Bhalamurugan GL, Valerie O, Mark L (2018) Valuable bioproducts obtained from microalgal biomass and their commercial applications: a review. Environ Eng Res 23:229–241CrossRefGoogle Scholar
  12. Bharathiraja B, Jayamuthunagai J, Sudharsana T, Bharghavia A, Praveenkumar R, Chakravarthy M, Yuvaraj D (2017) Biobutanol – an impending biofuel for future: a review on upstream and downstream processing techniques. Renew Sust Energy Rev 68:788–807CrossRefGoogle Scholar
  13. Bhuvaneswari K, Devika R (2005) Studies on the physico-chemical and biological characteristics of Coovum river. Asian J Microbiol Biotechnol Environ Sci 41:7449–7451Google Scholar
  14. Borowitzka MA (1998) Limits to growth. In: Wong YS, Tam NFY (eds) Wastewater treatment with algae. Springer-Verlag, Berlin, pp 203–226CrossRefGoogle Scholar
  15. Bruneel C, Lemaheiu C, FraeyeI, Ryckebosch E, Muylaert K, Buyse J, Foubert I (2013) Impact of microalgal feed supplementation on omega-3 fatty acid enrichment of hen eggs. J Funct Foods 5:897–904CrossRefGoogle Scholar
  16. Cai T, Park SY, Li Y (2013) Nutrient recovery from wastewater streams by microalgae: status and prospects. Renew Sust Energ Rev 19:360–369CrossRefGoogle Scholar
  17. Carvalho AP, Meireles LA, Malcata XF (2006) Microalgal reactors: a review of enclosed system designs and performances. Biotechnol Prog 22:1490–1506CrossRefGoogle Scholar
  18. Chanakya HUN, Mahapatra DM, Sarada R, Chauhan VS, Abitha R (2012) Sustainability of large-scale algal biofuel production in India. J Indian Inst Sci 92:63–98Google Scholar
  19. Chaudhary L, Pradhan P, Soni N, Singh P, Tiwari A (2014) Algae as a feedstock for bioethanol production: new entrance in biofuel world. Int J Chem Technol Res 6:1381–1389Google Scholar
  20. Chen YC (2003) Immobilized Isochrysis galbana (Haptophyta) for long-term storage and applications for feed and water quality control in clam (Meretrixlusoria) cultures. J Appl Phycol 15:439–444CrossRefGoogle Scholar
  21. Cheng HH, Whang LM, Chan KC, Chung MC, Wua SH, Liu CP, Tien SY, Chen SY, Chang JS, Lee WJ (2014) Biological butanol production from microalgae-based biodiesel residues by Clostridium acetobutylicum. Bioresour Technol 184:379–385CrossRefGoogle Scholar
  22. Chinnasamy S, Bhatnagar A, Hunt RW, Das KC (2010) Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. Bioresour Technol 101:3097–3105CrossRefGoogle Scholar
  23. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefGoogle Scholar
  24. Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends in Biotechnology 26(3):126–131CrossRefGoogle Scholar
  25. Choi HJ (2016) Dairy wastewater treatment using microalgae for potential biodiesel application. Environ Eng Res 21(4):393–400CrossRefGoogle Scholar
  26. Christensen L, Sims R (2011) Production and harvesting of microalgae for wastewater treatment, biofuels, and bioproducts. Biotechnol Adv 29:686–702CrossRefGoogle Scholar
  27. Crofcheck CL, Xinyi E, Shea AP, Monstross M, Crocker M, Andrews R (2012) Influence of media composition on the growth rate of Chlorella vulgaris and Scenedesmus acutus utilized for CO2 mitigation. J Biochem Technol 4:589–594Google Scholar
  28. Day JG, Fleck RA, Benson EE (1998) Cryopreservation of multicellular algae: problems and perspectives. Cryo Letters 19:205–206Google Scholar
  29. Demirel B, Yenigun O, Onay TT (2005) Anaerobic treatment of dairy wastewaters: a review. Process Biochem 40:2583–2595CrossRefGoogle Scholar
  30. Ding JF, Zhao FM, Cao YF, Xing L, Liu W, Mei S, Li SJ (2014) Cultivation of microalgae in dairy wastewater without sterilization. Int J Phytoremediation 17:222–227CrossRefGoogle Scholar
  31. Ding S, Chen M, Gong M, Fan X, Qin B, Xu H, Gao SS, Jin S, Daniel CW, Tsang C, Zhang C (2018) Internal phosphorus loading from sediments causes seasonal nitrogen limitation for harmful algal blooms. Sci Total Environ 625:872–884CrossRefGoogle Scholar
  32. Ebadi AG, Hisoriev H, Zarnegar M, Ahmadi H (2017) Hydrogen and syngas production by catalytic gasification of algal biomass (Cladophoraglomerata L.) using alkali and alkaline-earth metals compounds (AAEM). Environ Technol:1479–1487Google Scholar
  33. El-Sayed WMM, Ibrahim HAH, Abdul-Raouf UM, El-Nagar MM (2016) Evaluation of bioethanol production from Ulva lactuca by Saccharomyces cerevisiae. J Biotechnol Biomater 6:226CrossRefGoogle Scholar
  34. Evans AM, Smith DL, Moritz JS (2015) Effects of algae incorporation into broiler starter diet formulations on nutrient digestibility and 3 to 21 d bird performance. J Appl Poult Res 24:206–214CrossRefGoogle Scholar
  35. Fulke AB, Mudliar SN, Yadav R, Shekh AY, Srinivasan N, Ramanan R, Krishnamurthi K, Devi SS, Chakrabarti T (2010) Bio-mitigation of CO2, calcite formation and simultaneous biodiesel precursors production using Chlorella sp. Bioresour Technol 101:8473–8476CrossRefGoogle Scholar
  36. Gatrell S, Lum K, Kim J, Lei XG (2014) Nonruminant nutrition symposium: potential of defatted microalgae from the biofuel industry as an ingredient to replace corn and soybean meal in swine and poultry diets. J Anim Sci 92:1306–1314CrossRefGoogle Scholar
  37. Ghayal MS, Pandya MT (2013) Microalgae biomass: a renewable source of energy. Energy Procedia 32:242–250CrossRefGoogle Scholar
  38. Goncalves AL, Pires JCM, Simoes M (2017) A review on the use of microalgal consortia for wastewater treatment. Algal Res 24:403–415CrossRefGoogle Scholar
  39. Gough C (2008) State of the art in carbon dioxide capture and storage in the UK: an experts’ review. Int J Greenhouse Gas Control 2:155–168CrossRefGoogle Scholar
  40. Griffiths MJ, Harrison TL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493–507CrossRefGoogle Scholar
  41. Grobbelaar JU (1982) Potential of algal production. Water SA 8:79–85Google Scholar
  42. Grobbelaar JU (2004) Algal nutrition - mineral nutrition. In: Richmond A (ed) Handbook of sMicroalgal culture: biotechnology and applied phycology. Blackwell Science Ltd, Oxford, pp 3–19Google Scholar
  43. Guldhe A, Kumari S, Ramanna L, Ramsundar P, Singh P, Rawat I, Bux F (2017) Prospects, recent advancements and challenges of different wastewater streams for microalgal cultivation. J Environ Manag 203:299–315CrossRefGoogle Scholar
  44. Gülyurt MO, Özçimen D, Inan B (2016) Biodiesel production from Chlorella protothecoides oil by microwave-assisted Transesterification. Int J Mol Sci 17:579CrossRefGoogle Scholar
  45. Gupta H, Fan LS (2002) Carbonation–calcination cycle using high reactivity calcium oxide for carbon dioxide separation from flue gas. Ind Eng Chem Res 41:4035–4042CrossRefGoogle Scholar
  46. Gupta S, Pandey RA, Pawar SB (2016) Microalgal bioremediation of food-processing industrial wastewater under mixotrophic conditions: kinetics and scaleup approach. Front Chem Sci Eng 10(4):499–508CrossRefGoogle Scholar
  47. GuanHua Huang, Feng Chen, Dong Wei, XueWu Zhang, Gu Chen (2010) Biodiesel production by microalgal biotechnology. Applied Energy 87(1):38–46CrossRefGoogle Scholar
  48. Harun R, Danquah MK, Forde GM (2009) Microalgal biomass as a fermentation feedstock for bioethanol production. J Chem Technol Biotechnol 85:199–203Google Scholar
  49. He PJ, Mao B, Shen CM, Shao LM, Lee DJ, Chang JS (2013) Cultivation of Chlorella vulgaris on wastewater containing high levels of ammonia for biodiesel production. Bioresour Technol 129:177–181CrossRefGoogle Scholar
  50. Hellebust JA, Ahmad I (1989) Regulation of nitrogen assimilation in green microalgae. Biol Oceanogr 6:241–255Google Scholar
  51. Hempel N, Petrick I, Behrendt F (2012) Biomass productivity and productivity of fatty acids and amino acids of microalgae strains as key characteristics of suitability for biodiesel production. J Appl Phycol 24:1407–1418CrossRefGoogle Scholar
  52. Hena S, Fatimah S, Tabassum S (2015) Cultivation of algae consortium in a dairy farm wastewater for biodiesel production. Water Resour Ind 10:1–14CrossRefGoogle Scholar
  53. Hernandez D, Riano B, Coca M, Garcia-Gonzalez MC (2015) Saccharification of carbohydrates in microalgal biomass by physical, chemical and enzymatic pre-treatments as a previous step for bioethanol production. Chem Eng J 262:939–945CrossRefGoogle Scholar
  54. Ho SH, Shu-Huang W, Chen CY, Hasunuma T, Kondo A, Chang JS (2013) Bioethanol production using carbohydrate-rich microalgae biomass as feedstock. Bioresour Technol 135:191–198CrossRefGoogle Scholar
  55. Hodaifa G, Martínez ME, Sánchez S (2009) Daily doses of light in relation to the growth of Scenedesmus obliquus in diluted three-phase olive mill wastewater. J Chem Technol Biotechnol 84:1550–1558CrossRefGoogle Scholar
  56. Hosseinia NS, Shang H, Scott JA (2018) Biosequestration of industrial off-gas CO2 for enhanced lipid productivity in open microalgae cultivation systems. Renew Sust Energy Rev 92:458–469CrossRefGoogle Scholar
  57. Hu Z, Ma X, Li L (2013) The characteristic and evaluation method of fast pyrolysis of microalgae to produce syngas. Bioresour Technol 140:220–226CrossRefGoogle Scholar
  58. Hu Z, Ma X, Li L, Wu J (2014) The catalytic pyrolysis of microalgae to produce syngas. Energy Convers Manag 85:545–550CrossRefGoogle Scholar
  59. Hwang J, Kabra A, Ji M, Choi J, El-Dalatony M, Jeon B (2016) Enhancement of continuous fermentative bioethanol production using combined treatment of mixed microalgal biomass. Algal Res 17:14–20CrossRefGoogle Scholar
  60. IPCC, Intergovernmental Panel on Climate Change (2014) The scientific basis.
  61. Jambo SA, Abdulla R, Mohd Azhar SH, Marbawi H, Gansau JA, Ravindra P (2016) A review on third generation bioethanol feedstock. Renew Sust Energ Rev 65:756–769CrossRefGoogle Scholar
  62. John RP, Anisha GS, Nampoothiri KM, Pandey A (2011) Micro and macroalgal biomass: a renewable source for bioethanol. Bioresour Technol 102:186–193CrossRefGoogle Scholar
  63. Johnson MB, Wen Z (2010) Development of an attached microalgal growth system for biofuel production. Appl Microbiol Biotechnol 85:525–534CrossRefGoogle Scholar
  64. Jorquera O, Kiperstok A, Sales EA, Embiruçu M, Ghirardi ML (2010) Comparative energy life-cycle analyses of microalgal biomass production in open ponds and photobioreactors. Bioresour Technol 101:1406–1413CrossRefGoogle Scholar
  65. Kotasthane (2017) Potential of microalgae for sustainable biofuel production. J Marine Sci Res Dev 7:2CrossRefGoogle Scholar
  66. Kothari R, Prasad R, Kumar V, Singh DP (2013) Production of biodiesel from microalgae Chlamydomonas polypyrenoideum grown on dairy industry wastewater. Bioresour Technol 144:499–503CrossRefGoogle Scholar
  67. Kunjapur AM, Bruce R (2010) Photobioreactor design for commercial biofuel production from Microalgae. Ind Eng Chem Res 49:3516–3526CrossRefGoogle Scholar
  68. Lam MK, Lee KT (2012) Microalgae biofuels: a critical review of issues, problems and the way forward. Biotechnol Adv 30:673–690CrossRefGoogle Scholar
  69. Lavric L, Cerar A, Fanedl L, Lazar B, Zitnik M, Logar RM (2017) Thermal pretreatment and bioaugmentation improve methane yield of microalgal mix produced in thermophilic anaerobic digestate. Anaerobe 46:162–169CrossRefGoogle Scholar
  70. Lee DH (2016) Levelized cost of energy and financial evaluation for biobutanol, algal biodiesel and biohydrogen during commercial development. Int J Hydrog Energy 41:2158–2159Google Scholar
  71. Levine RB, Costanza-Robinson MS, Spatafora GA (2011) Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production. Biomass Bioenergy 34:40–49CrossRefGoogle Scholar
  72. Liu Z, Campbell V, Heidelberg KB, Caron DA (2016) Gene expression demonstrates different nutritional strategies among three mixotrophicprotists. FEMS Microbiol Ecol 92:fiw106CrossRefGoogle Scholar
  73. Maizatul AY, Radin M, Mohamed SR, Adel A, Al-Gheethi MK, Hashim A (2017) An overview of the utilisation of microalgae biomass derived from nutrient recycling of wet market wastewater and slaughter house wastewater. Int Aquat Res 9:177–193CrossRefGoogle Scholar
  74. Makareviciene V, Skorupskaite V, Andruleviciute V (2013) Biodiesel fuel from microalgae-promising alternative fuel for the future: a review. Rev Environ Sci Biotechnol 12:119–130CrossRefGoogle Scholar
  75. Malla FA, Fayaz A, Khan SA, Rashmi, Sharma GK, Gupta N, Abraham G (2015) Phycoremediation potential of Chlorella minutissima on primary and tertiary treated wastewater for nutrient removal and biodiesel production. Ecol Eng 75:343–349CrossRefGoogle Scholar
  76. Martinez M, Jimenez J, Yousfi FEI (1999) Influence of phosphorus concentration and temperature on growth and phosphorus uptake by the microalga Scenedesmus obliqus. Bioresour Technol 67:233–240CrossRefGoogle Scholar
  77. Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew sust energy rev 14:217–232CrossRefGoogle Scholar
  78. Mobin S, Alam F (2014) Third generation biofuel from algae. Procedia Eng 105:763–768Google Scholar
  79. Mulbry W, Kondrad S, Buyer J (2008a) Treatment of dairy and swine manure effluents using freshwater algae: fatty acid content and composition of algal biomass at different manure loading rates. J Appl Phycol 20:1079–1085CrossRefGoogle Scholar
  80. Mulbry W, Kondrad S, Pizarro C, Elizabeth KW (2008b) Treatment of dairy manure effluent using freshwater algae: algal productivity and recovery of manure nutrients using pilot-scale algal turf scrubbers. Bioresour Technol 99:8137–8142CrossRefGoogle Scholar
  81. National Algal Biofuels Technology Review (2016) U.S. Department of Energy, Office of Energy Efficiency and Renewable EnergyGoogle Scholar
  82. Niess D, Reisser W, Wiessner (1981) The role of endosymbiotic algae in photoaccumulation of green Paramecium bursaria. Planta 152(3):268–271CrossRefGoogle Scholar
  83. Oswald WJ (1988) Micro-algae and waste-water treatment. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University press, Cambridge, pp 305–328Google Scholar
  84. Passos F, Astals S, Ferrer I (2013) Anaerobic digestion of microalgal biomass after ultrasound pretreatment. Waste Manag 34:2098–2103CrossRefGoogle Scholar
  85. Picardo MC, Medeiros JL, Queiroz FAO, Chaloub RM (2013) Effects of CO2 enrichment and nutrients supply intermittency on batch cultures of Isochrysis galbana. Bioresour Technol 143:242–250CrossRefGoogle Scholar
  86. Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102:17–12CrossRefGoogle Scholar
  87. Popp J, Lakner Z, Harangi-Rákos M, Fári M (2014) The effect of bioenergy expansion: food, energy, and environment. Renew Sust Energ Rev 32:559–578CrossRefGoogle Scholar
  88. Qin L, Shu Q, Wang Z, Shang C, Zhu S, Xu J, Li R, Zhu R, Yuan Z (2013) Cultivation of Chlorella vulgaris in dairy wastewater pretreated by UV irradiation and sodium hypochlorite. Appl Biochem Biotechnol 172:1121–1130CrossRefGoogle Scholar
  89. Raheem A, Azlina W, Taufiq Y, Michael KD, Razif H (2015) Optimization of the microalgae Chlorella vulgaris for syngas production using central composite design. RSC Adv 88:71805–71815CrossRefGoogle Scholar
  90. Raheem A, Dupont V, Channa AK, Zhao X, Vuppaladadiyam AK, Yun-Hin, Taufiq-Yap, Zhao M, Harun R (2017) Parametric characterisation of air gasification of Chlorella vulgaris biomass. Energy Fuel 31:2959–2969CrossRefGoogle Scholar
  91. Ramjeawon, Cleaner T (2000) Production in Mauritian cane-sugar factories. J Clean Prod 8:503–510CrossRefGoogle Scholar
  92. Reyimu Z, OzcImen D (2017) Batch cultivation of marine microalgae Nannochloropsis oculata and Tetraselmis suecica in treated municipal wastewater toward bioethanol production. J Clean Prod 150:40–46CrossRefGoogle Scholar
  93. Rhee G-Y (1978) Effects of N:P atomic ratios and nitrate limitation on algal growth, cell composition and nitrate uptake. Limnol Oceanogr 23:1CrossRefGoogle Scholar
  94. Rizza LS, Smachetti MES, Nascimento MD, Salerno GL, Curatti L (2017) Bioprospecting for native microalgae as an alternative source of sugars for the production of bioethanol. Algal Res 22:140–147CrossRefGoogle Scholar
  95. Rubio CF, Fernandez FG, Perez JA, Camacho FG, Grima EM (1999) Prediction of dissolved oxygen and carbon dioxide concentration profiles in tubular photobioreactors for microalgal culture. Biotechnol Bioeng 62:71–86CrossRefGoogle Scholar
  96. Richmond A (2013) Biological principles of mass cultivation of photoautotrophic microalgae. In: Richmond A, Hu Q (eds) Handbook of microalgal culture: applied phycology and biotechnology, 2nd edn. John Wiley & Sons, Chichester, pp 171–204Google Scholar
  97. Sheehan J, Dunahay T, Benemann J, Roessler P (1998) A look back at the U.S. Department of Energy’s Aquatic Species Program — biodiesel from algae. National Renewable Energy Laboratory, Golden, CO. Report NREL/TP-580-2419Google Scholar
  98. Shekh AY, Shrivastava P, Krishnamurthi K, Mudliar SN, Devi SS, Kanade GS, Lokhande K, Chakrabati T (2013a) Stress-induced lipids are unsuitable as a direct biodiesel feedstock: a case study with Chlorella pyrenoidosa. Bioresour Technol 138:382–386CrossRefGoogle Scholar
  99. Shekh AY, Krishnamurthi K, Yadav RR et al (2013b) Algae-mediated carbon dioxide sequestration for climate change mitigation and conversion to value- added products. In: Faizal B (ed) Biotechnological application of microalgae: biodiesel and value added products. CRC press, New York, pp 161–178CrossRefGoogle Scholar
  100. Shekh AY, Shrivastava P, Krishnamurthi K, Mudliar SN, Devi SS, Kanade GS, Chakrabati T (2016a) Stress enhances poly-unsaturation rich lipid accumulation in Chlorella sp. and Chlamydomonas sp. Biomass Bioenergy 84:59–66CrossRefGoogle Scholar
  101. Shekh AY, Shrivastava P, Gupta A, Krishnamurthi K, Devi SS, Mudliar SN (2016b) Biomass and lipid enhancement in Chlorella sp. with emphasis on biodiesel quality assessment through detailed FAME signature. Bioresour Technol 201:276–286CrossRefGoogle Scholar
  102. Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications ofmicroalgae. J Biosci Bioeng 101(2):87–96CrossRefGoogle Scholar
  103. Stephenson AL, Dennis JS, Christopher JH, Stuart AS, Alison GS (2010) Influence of nitrogen-limitation regime on the production by Chlorella vulgaris of lipids for biodiesel feedstocks. Biofuels 1:47–58CrossRefGoogle Scholar
  104. Sugiharto S, Lauridsen C (2016) Dietary Chlorella supplementation effect on immune responses and growth performances of broiler chickens exposed to post hatch holding time. Livest Res Rural Dev 28:119Google Scholar
  105. Sydney EB, Novak AC, Carvalho JC, Soccol CR (2014) Respirometric balance and carbon fixation of industrially important algae. In: Pandey A, Lee DJ, Chisti Y, Soccol CR (eds) Biofuels from algae. Elsevier, Burlington, pp 67–84CrossRefGoogle Scholar
  106. Tian C, Li B, Liu Z, Lu H (2014) Chem inform abstract: hydrothermal liquefaction for algal biorefinery: a critical review. Renew Sust Energ Rev 38:933–950CrossRefGoogle Scholar
  107. Uchiyama H, Aoyagi H, Ugwu CU (2008) Photobioreactors for mass cultivation of algae. Bioresour Technol 99:4021–4028CrossRefGoogle Scholar
  108. Wahal S, Viamajala S (2016) Uptake of inorganic and organic nutrient species during cultivation of a Chlorella isolate in anaerobically digested dairy waste. Biotechnol Prog 32:1336–1342CrossRefGoogle Scholar
  109. Wang Y, Guoa WQ, Lob YC, Chang JS, Rena NQ (2014) Characterization and kinetics of bio-butanol production with Clostridium acetobutylicum ATCC824 using mixed sugar medium simulating microalgae-based carbohydrates. Biochem Eng J 91:220–230CrossRefGoogle Scholar
  110. Wang Y, Ho SH, Cheng CL, Nagarajan D, Guo WQ, Lin C, Li S, Ren N, Chang JS (2017) Nutrients and COD removal of swine wastewater with an isolated microalgal strain Neochloris aquatica CL-M1 accumulating high carbohydrate content used for biobutanol production. Bioresour Technol 242:7–14CrossRefGoogle Scholar
  111. Weidong L, Wang Z, Wang X, Yuan Z (2015) Cultivation of Chlorella sp. using raw diary wastewater for nutrient removal and biodiesel production: characteristics comparison of indoor bench-scale and outdoor pilot-scale cultures. Bioresour Technol 192:382–388CrossRefGoogle Scholar
  112. Weissman JC, Goebel RP, Benemann JR (1988) Photobioreactor design: mixing, carbon utilization, and oxygen accumulation. Biotechnol Bioeng 31:336–344CrossRefGoogle Scholar
  113. Yadavalli R, Rao CS, Rao RS, Potumarthi R (2014) Dairy effluent treatment and lipids production by Chlorella pyrenoidosa and Euglena gracilis: study on open and closed systems. Asia Pac J Chem Eng 9:368–373CrossRefGoogle Scholar
  114. Yellapua SK, Bharati, Kaur R, Kumar RL, Tiwari B, Zhang X, Tyagi RD (2018) Recent developments of downstream processing for microbial lipids and conversion to biodiesel. Bioresour Technol 256:515–528CrossRefGoogle Scholar
  115. Yoo C, Jun SY, Lee JY, Ahn CY, Oh HM (2010) Selection of microalgae for lipid production under high levels carbon dioxide. Bioresour Technol 101:71–74CrossRefGoogle Scholar
  116. Zhou W, Chen P, Min M, Ma X, Wang J, Griffith R, Hussain F, Peng P, Xie Q, Li Y, Shi J, Meng J, Ruan R (2014) Environment-enhancing algal biofuel production using wastewaters. Renew Sust Energ Rev 36:256–269CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Jitendra Mehar
    • 1
  • Ajam Shekh
    • 1
  • Nethravathy Uthaiah Malchira
    • 1
  • Sandeep Mudliar
    • 1
  1. 1.Plant Cell Biotechnology DepartmentCSIR-Central Food Technological Research InstituteMysoreIndia

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