Potential Biotechnological Applications of Microalgae Grown in Wastewater: A Holistic Approach

  • Amit Kumar Singh
  • Abhay K. Pandey


Industrial revolution and population burst have turned out to be the major causes of environmental pollution. Economic growth of countries depends on industrial development which is a major environment polluter as they discharge their wastes into the nearby waterbodies. Another burning issue for the world is fossil fuel scarcity and increased discharge of greenhouse gases resulting in climate change. Therefore researchers around the world are searching for an eco-friendly tool that depollutes wastewater in addition to providing alternatives to the fossil fuel. Microalgae appear to be a feasible option for this purpose. The current chapter describes in detail the composition of wastewater, phycoremediation, nutrient and heavy metal uptake mechanism by microalgae, wastewater utilisation for the cost-effective biofuel production and finally utilisation of their biomass for other commercial purposes such as in food industry, health sector and cosmetic industry.


Microalgae Wastewater Biofuel Nutrients Pharmaceuticals Cosmetics 



Amit Kumar Singh acknowledges financial support in the form of Senior Research Fellowship from CSIR New Delhi, India. The authors also acknowledge UGC-SAP and DST-FIST facilities of Biochemistry Department, University of Allahabad, Allahabad.


  1. Abdel-Raouf N, Al-Homaidan AA, Ibraheem IBM (2012) Microalgae and wastewater treatment. Saudi J Biol Sci 19:257–275Google Scholar
  2. Acien FG, Fernandez JM, Magan JJ, Molina E (2012) Production cost of a real microalgae production plant and strategies to reduce it. Biotechnol Adv 30:1344–1353CrossRefGoogle Scholar
  3. Ahluwalia SS, Goyal D (2007) Microbial and plant derived biomass for removal of heavy metals from wastewater. Bioresour Technol 98:2243–2257CrossRefGoogle Scholar
  4. Ambrosi MA, Reinehr CO, Bertolin TE, Costa JA, Colla LM (2008) Health properties of Spirulina spp. J Basic Appl Pharma Sci 29(2):109–117Google Scholar
  5. Ashton B, Hill K, Piazza A, Zeitz R (1984) Famine in China, 195861. Popu Dev Rev 10:613–645CrossRefGoogle Scholar
  6. Becker W (2004) Microalgae in human and animal nutrition. In: Richmond A (ed) Handbook of microalgal culture. Blackwell, Oxford, pp 312–351Google Scholar
  7. Bhagavathy S, Sumathi P, Jancy I, Bell S (2011) Green algae Chlorococcum humicola - a new source of bioactive compounds with antimicrobial activity. Asian Pac J Trop Biomed 1:S1–S7CrossRefGoogle Scholar
  8. Bhargava A, Carmona FF, Bhargava M, Srivastava S (2012) Approaches for enhanced phytoextraction of heavy metals. J Environ Manag 105:103–120CrossRefGoogle Scholar
  9. Bixler HJ, Porse H (2011) A decade of change in the seaweed hydrocolloids industry. J Appl Phycol 23:321–335CrossRefGoogle Scholar
  10. Borowitzka MA (2013) High-value products from microalgae-their development and commercialisation. J Appl Phycol 25:743–756CrossRefGoogle Scholar
  11. Cai T, Stephen Y, Park YL (2013) Nutrient recovery from waste water streams by microalgae: status and prospects. Renew Sust Energ Rev 19:360–369CrossRefGoogle Scholar
  12. Carolin CF, Kumar PS, Saravanan A et al (2017) Efficient techniques for the removal of toxic heavy metals from aquatic environment: a review. J Environ Chem Eng 5:2782–2799CrossRefGoogle Scholar
  13. Chaput G, Charmanski K, Farag I (2012) Sustainable production of microalgae oil feedstock using municipal wastewater and CO2 fertilization. Int J Eng Sci Technol 4:3489–3499Google Scholar
  14. Chinnasamy S, Bhatnagar A, Hunt RW, Das KC (2010) Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. Bioresour Technol 101:3097–3105CrossRefGoogle Scholar
  15. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306CrossRefGoogle Scholar
  16. Colak O, Kaya Z (1988) A study on the possibilities of biological wastewater treatment using algae. Doga Biyolji Serisi 12(1):18–29Google Scholar
  17. Colla LM, Reinehr CO, Reichert C, Costa JAV (2007) Production of biomass and nutraceutical compounds by Spirulina platensis under different temperature and nitrogen regimes. Bioresour Technol 98(7):1489–1493CrossRefGoogle Scholar
  18. Costa JA, Morais MG (2013) Microalgae for food production. In: Soccol CR, Pandey A, Larroche C (eds) Fermentation process engineering in the food industry. Taylor & Francis, Boca Raton, p 486Google Scholar
  19. Costanzo SD, O’Donohue MJ, Dennison WC et al (2001) A new approach for detecting and mapping sewage impacts. Mar Pollut Bull 42:149–156CrossRefGoogle Scholar
  20. Davidson K, Gowen RJ, Tett P (2012) Harmful algal blooms: how strong is the evidence that nutrient ratios and forms influence their occurrence? Estuar Coast Shelf Sci 115:399–413CrossRefGoogle Scholar
  21. Dean AP, Sigee DC, Estrada B, Pittman JK (2010) Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae. Bioresour Technol 101:4499–4507CrossRefGoogle Scholar
  22. Dominguez H (2013) Functional ingredients from algae for foods and nutraceuticals. Elsevier. ISBN 9780857098689Google Scholar
  23. Duenas JF, Alonso JR, Rey ÀF, Ferrer AS (2003) Characterisation of phosphorous forms in wastewater treatment plants. J Hazard Mater 97:193–205CrossRefGoogle Scholar
  24. Ferreira A, Herpin U, Monteiro A (2007) Agricultural use of treated sewage effluents: agronomic and environmental implications and perspectives for Brazil. Sci Agric 64:194–209CrossRefGoogle Scholar
  25. Francavilla M, Trotta P, Luque R (2010) Phytosterols from Dunaliella tertiolecta and Dunaliella salina: a potentially novel industrial application. Bioresour Technol 101(11):4144–4150CrossRefGoogle Scholar
  26. Gochfeld M (2003) Cases of mercury exposure, bioavailability, and adsorption. Ecotoxicol Environ Saf 56:174–179CrossRefGoogle Scholar
  27. Graneli E, Weberg M, Salomon PS (2008) Harmful algal blooms of allelopathic microalgal species: the role of eutrophication. Harmful Algae 8:94–102CrossRefGoogle Scholar
  28. Griffiths MJ, Harrison STL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493–507CrossRefGoogle Scholar
  29. Guo T, Englehardt J, Wu T (2015) Review of cost versus scale: water and wastewater treatment and reuse processes. Water Sci Technol 69:223–234CrossRefGoogle Scholar
  30. Harun R, Singh M, Forde GM, Danquah MK (2010) Bioprocess engineering of microalgae to produce a variety of consumer products. Renew Sust Energ Rev 14(3):1037–1047CrossRefGoogle Scholar
  31. Hills C, Nakamura H (1978) Food from sunlight. World Hunger Research Publ, Boulder Creek, CAGoogle Scholar
  32. Horikoshi T, Nakajima A, Sakaguchi T (1981) Studies on the accumulation of heavy metal elements in biological systems-XIX. Accumulation of uranium by microorganisms. Eur J Appl Microbiol Biotechnol 12:90–96CrossRefGoogle Scholar
  33. Huang GH, Chen F, Wei D, Zhang XW, Chen G (2010) Biodiesel production by microalgal biotechnology. Appl Energy 87:38–46CrossRefGoogle Scholar
  34. Ibanez E, Cifuentes A (2013) Benefits of using algae as natural sources of functional ingredients. J Sci Food Agric 93(4):703–709CrossRefGoogle Scholar
  35. Jain R, Raghukumar S, Tharanathan R, Bhosle NB (2005) Extracellular polysaccharide production by thraustochytrid protists. Mar Biotechnol 7:184–192CrossRefGoogle Scholar
  36. Jea JY, Park PJ, Kim EK, Park JS, Yoon HD (2009) Antioxidant activity of enzymatic extracts from the brown seaweed Undaria pinnatifida by electron spin resonance spectroscopy. Food Sci Technol 42:874–878Google Scholar
  37. Kelly MG, Whitton BA (1995) The trophic diatom index: a new index for monitoring eutrophication in rivers. J Appl Phycol 7:433–444CrossRefGoogle Scholar
  38. Kim SK, Ravichandran YD, Khan SB, Kim YT (2008) Prospective of the cosmeceuticals derived from marine organisms. Biotechnol Bioprocess Eng 13:511–523CrossRefGoogle Scholar
  39. Lau PS, Tam NFY, Wang YS (1995) Effect of algal density on nutrient removal from primary settled wastewater. Environ Pollut 89:56–66CrossRefGoogle Scholar
  40. Lim S, Chu W, Phang S (2010) Use of Chlorella vulgaris for bioremediation of textile wastewater. J Bioresour Technol 101:7314–7322CrossRefGoogle Scholar
  41. Liu Y, Cao Q, Luo F, Chen J (2009) Biosorption of Cd2+, Cu2+, Ni2+ and Zn2+ ions from aqueous solutions by pretreated biomass of brown algae. J Hazard Mater 163:931–938CrossRefGoogle Scholar
  42. Madkour FF, Abdel-Daim MM (2013) Hepatoprotective and antioxidant activity of Dunaliella salina in paracetamol-induced acute toxicity in rats. Indian J Pharm Sci 75(6):642–648Google Scholar
  43. Markou G, Nerantzis E (2013) Microalgae for high-value compounds and biofuels production: a review with focus on cultivation under stress conditions. Biotechnol Adv 31(8):1532–1542CrossRefGoogle Scholar
  44. Marti E, Aumatell J, Gode L (2001) Nutrient retention efficiency in streams receiving inputs from wastewater treatment plants. J Environ Qual 33:285–293CrossRefGoogle Scholar
  45. Meybeck M (1982) Carbon, nitrogen, and phosphorus transport by world rivers. Am J Sci 282:401–450CrossRefGoogle Scholar
  46. Mishra N, Panda PK, Parida BK, Mishra BK (2016) Way forward to achieve sustainable and cost-effective biofuel production from microalgae: a review. Int J Env Sci Tech 13:2735–2756CrossRefGoogle Scholar
  47. Mulbry W, Kondrad S, Pizarro C, Kebede WE (2008) Treatment of dairy manure effluent using fresh water algae: algal productivity and recovery of manure nutrients using pilot-scale algal turf scrubbers. Bioresour Technol 99:8137–8142CrossRefGoogle Scholar
  48. Munoz R, Guieysse B (2008) Algal–bacterial processes for the treatment of hazardous 755 contaminants: a review. Water Res 40:2799–2815CrossRefGoogle Scholar
  49. Nizard C, Friguet B, Moreau M, Bulteau AL, Saunois A (2007) Use of phaeodactylum algae extract as cosmetic agent promoting the proteasome activity of skin cells and cosmetic composition comprising same, US patent (US7220417B2). Google Scholar
  50. Olgum EJ (2003) Phycoremediation: key issues for cost-effective nutrient removal processes. Biotechnol Adv 22:81–91CrossRefGoogle Scholar
  51. Oswald WJ, Gotaas HB (1957) Photosynthesis in sewage treatment. Trans Am Soc Civil Eng 122:73–105Google Scholar
  52. O’Neil JM, Davis TW, Burford MA, Gobler CJ (2012) The rise of harmful cyanobacteria blooms: the potential roles of eutrophication and climate change. Harmful Algae 14:313–334CrossRefGoogle Scholar
  53. Pandi M, Shashirekha V, Swamy M (2009) Biosorption of chromium from retan chrome liquor by cyanobacteria. Microbiol Res 164:420–428CrossRefGoogle Scholar
  54. Priyadarshani I, Rath B (2012) Commercial and industrial applications of micro algae - a review. J Algal Biomass Util 3:89–100Google Scholar
  55. Przytocka-Jusiak M, Duszota M, Matusiak K, Mycielski R (1984) Intensive culture of Chlorella vulgaris/AA as the second stage of biological purification of nitrogen industry wastewaters. Water Res 18:1–7CrossRefGoogle Scholar
  56. Pulz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 65:635–648CrossRefGoogle Scholar
  57. Quinn JC, Davis R (2016) The potentials and challenges of algae based biofuels: a review of the techno-economic, life cycle, and resource assessment modeling. Bioresour Technol 184:444–452CrossRefGoogle Scholar
  58. Raja R, Hemaiswarya S, Kumar NA, Sridhar S, Rengasamy R (2008) A perspective on the biotechnological potential of microalgae. Crit Rev Microbiol 34:77–88CrossRefGoogle Scholar
  59. Rai UN, Singh NK, Verma S, Prasad D, Upadhyay AK (2011) Perspectives in plant based management of Ganga water pollution: a negative carbon technique to rehabilitate river ecosystem. Appl Bot Abs 31(1):64–81Google Scholar
  60. Rawat I, Ranjith RK, Mutanda T, Bux F (2011) Dual role of microalgae: Phycoremediation of domestic wastewater and biomass production for sustainable biofuels production. Appl Energy 88:3411–3424CrossRefGoogle Scholar
  61. Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100–112CrossRefGoogle Scholar
  62. Rosenberg JN, Oyler GA, Wilkinson L, Betenbaugh MJ (2008) A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution. Curr Opin Biotechnol 19:430–436CrossRefGoogle Scholar
  63. Ruiz G, Jeison D, Chamy R (2003) Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration. Water Res 37:1371–1377CrossRefGoogle Scholar
  64. Saidani K, Bedjou F, Benabdesseiam F, Touati N (2012) Antifungal activity of methanolic extracts of four Algerian marine algae species. Afr J Biotechnol 11:9496–9500CrossRefGoogle Scholar
  65. Sajilata MG, Singhal RS, Kamat MY (2008) Fractionation of lipids and purification of ã-linolenic acid (GLA) from Spirulina platensis. Food Chem 109(3):580–586CrossRefGoogle Scholar
  66. Sarkar B, Chakrabarti PP, Vijaykumar A, Kale V (2006) Wastewater treatment in dairy industries-possibility of reuse. Desalination 195(1–3):141–152CrossRefGoogle Scholar
  67. Shick JM, Dunlap WC (2002) Mycosporine-like amino acids and related gadusols: biosynthesis, accumulation, and UV-protective functions in aquatic organisms. Annu Rev Physiol 64:223–262CrossRefGoogle Scholar
  68. Singh L, Pavankumar AR, Lakshmanan R (2012) Effective removal of Cu2+ ions from aqueous medium using alginate as biosorbent. Ecol Eng 38:119–124CrossRefGoogle Scholar
  69. Singh AK, Ganguly R, Kumar S, Pandey AK (2017) Microalgae: a source of Nutraceuticals and industrial product. In: Abidi MM, Ansari MI, Maheshwari RK (eds) Molecular biology and pharmacognosy of beneficial plant. Lenin media private limited, Delhi. ISBN: 978–93–85995-56-9, pp 37–51Google Scholar
  70. Singh AK, Pandey AK (2018) Microalgae: an eco-friendly tool for the industrial wastewater treatment and biofuel production. In: Recent Advances in Environmental Management (Ed. RN Bhargava) pp 167–196, CRC Press, Taylor & Francis Group, Boca Raton, FL 33487-2742, USAGoogle Scholar
  71. Smee DF, Bailey KW, Wong MH, O’Keefe BR, Gustafson KR, Mishin VP (2008) Treatment of influenza A (H1N1) virus infections in mice and ferrets with cyanovirin-N. Antivir Res 80(3):266–271CrossRefGoogle Scholar
  72. Smith VH, Schindler DW (2009) Eutrophication science: where do we go from here? Trends Ecol Evol 24:201–207CrossRefGoogle Scholar
  73. Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101(2):87–96CrossRefGoogle Scholar
  74. Umamaheswari J, Shanthakumar S (2016) Efficacy of microalgae for industrial wastewater treatment a review on operating conditions, treatment efficiency and biomass productivity. Rev Environ Sci Biotechnol 15:265–284CrossRefGoogle Scholar
  75. Venugopal V (2009) Marine products for healthcare. CRC Press, Boca RatonGoogle Scholar
  76. Verdy C, Branka JE, Mekideche N (2011) Quantitative assessment of lactate and progerin production in normal human cutaneous cells during normal ageing: effect of an Alaria esculenta extract. Int J Cosmet Sci 33:462–466CrossRefGoogle Scholar
  77. Wu LF, Chen PC, Huang AP, Lee CM (2012) The feasibility of biodiesel production by microalgae using industrial wastewater. Bioresour Technol 113:14–18CrossRefGoogle Scholar
  78. Wu XF, Kosaric N (1991) Removal of organochlorine compounds in an upflow flocculated algae photo-bioreactor Wat. Sci Technol 24:221–232Google Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Amit Kumar Singh
    • 1
  • Abhay K. Pandey
    • 1
  1. 1.Department of BiochemistryUniversity of AllahabadAllahabadIndia

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