Citrus Oils

  • Ali Osman


Citrus essential oils (EOs) has been identified in different parts of fruits as well as in leaves (particularly present in fruit flavedo), showing that limonene, β-myrcene, α-pinene, p-cymene, β-pinene, terpinolene, and other elements are the major aromatic compounds of many citrus species. EOs from citrus fruits are a rich source of bio-functional compounds with various health properties, including antioxidant, antimicrobial, anti-inflammatory, and cytoprotective activities. They are mixtures of more than 200 components that can be grouped into 2 fractions which contain monoterpenes and sesquiterpenes hydrocarbons as well as their oxygenated derivatives along with aliphatic aldehydes, alcohols and esters. Today citrus fruit, juice or peel oils are currently used in everything from food or food preparations, soft drinks, ice cream, candy, pharmaceutical preparations, air fresheners, cleaning products, solvents as well as colognes and fine perfumes. This chapter will cover the production, chemistry of citrus essential oils and its applications.


Essential oil Limonene Antioxidant Antimicrobial Cytoprotective activity 


  1. Ademosun, A. O., Oboh, G., Olupona, A. J., Oyeleye, S. I., Adewuni, T. M., & Nwanna, E. E. (2016). Comparative study of chemical composition, in vitro inhibition of cholinergic and monoaminergic enzymes, and antioxidant potentials of essential oil from peels and seeds of sweet orange (Citrus sinensis [L.] Osbeck) fruits. Journal of Food Biochemistry, 40, 53–60.CrossRefGoogle Scholar
  2. Ahmad, M. M., Iqbal, Z., Anjum, F., & Sultan, J. (2006). Genetic variability to essential oil composition in four citrus fruit species. Pakistan Journal of Botany, 38, 319.Google Scholar
  3. Akpinar, B. (2005). The effects of olfactory stimuli on scholastic performance. The Irish Journal of Education/Iris Eireannach an Oideachais, 36, 86–90.Google Scholar
  4. Alfonzo, A., Martorana, A., Guarrasi, V., Barbera, M., Gaglio, R., Santulli, A., Settanni, L., Galati, A., Moschetti, G., & Francesca, N. (2017). Effect of the lemon essential oils on the safety and sensory quality of salted sardines (Sardina pilchardus Walbaum 1792). Food Control, 73, 1265–1274.CrossRefGoogle Scholar
  5. Ali, N. A. A., Chhetri, B. K., Dosoky, N. S., Shari, K., Al-Fahad, A. J., Wessjohann, L., & Setzer, W. N. (2017). Antimicrobial, antioxidant, and cytotoxic activities of Ocimum forskolei and Teucrium yemense (Lamiaceae) essential oils. Medicines, 4, 17.PubMedCentralCrossRefPubMedGoogle Scholar
  6. AL-Jabri, N. N., & Hossain, M. A. (2014). Comparative chemical composition and antimicrobial activity study of essential oils from two imported lemon fruits samples against pathogenic bacteria. Beni-Suef University Journal of Basic and Applied Sciences, 3, 247–253.CrossRefGoogle Scholar
  7. AL-Jabri, N. N., & Hossain, M. A. (2018). Chemical composition and antimicrobial potency of locally grown lemon essential oil against selected bacterial strains. Journal of King Saud University-Science, 30, 14–20.CrossRefGoogle Scholar
  8. Ammar, A. H., Bouajila, J., Lebrihi, A., Mathieu, F., Romdhane, M., & Zagrouba, F. (2012). Chemical composition and in vitro antimicrobial and antioxidant activities of Citrus aurantium L. flowers essential oil (Neroli oil). Pakistan Journal of Biological Sciences, 15, 1034–1040.PubMedCrossRefGoogle Scholar
  9. Amorim, J. L., Simas, D. L. R., Pinheiro, M. M. G., Moreno, D. S. A., Alviano, C. S., da Silva, A. J. R., & Fernandes, P. D. (2016). Anti-inflammatory properties and chemical characterization of the essential oils of four citrus species. PLoS One, 11, e0153643.PubMedPubMedCentralCrossRefGoogle Scholar
  10. Asjad, H. M. M., Akhtar, M. S., Bashir, S., Din, B., Gulzar, F., Khalid, R., & Asad, M. (2013). Phenol, flavonoid contents and antioxidant activity of six common citrus plants in Pakistan. Journal of Pharmaceutical and Cosmetic Sciences, 1, 1–5.Google Scholar
  11. Attaway, J. A., Pieringer, A. P., & Barabas, L. J. (1967). The origin of citrus flavor components—III.: A study of the percentage variations in peel and leaf oil terpenes during one season. Phytochemistry, 6, 25–32.CrossRefGoogle Scholar
  12. Azmir, J., Zaidul, I., Rahman, M., Sharif, K., Mohamed, A., Sahena, F., Jahurul, M., Ghafoor, K., Norulaini, N., & Omar, A. (2013). Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering, 117, 426–436.CrossRefGoogle Scholar
  13. Belsito, E. L., Carbone, C., Di Gioia, M. L., Leggio, A., Liguori, A., Perri, F., Siciliano, C., & Viscomi, M. C. (2007). Comparison of the volatile constituents in cold-pressed bergamot oil and a volatile oil isolated by vacuum distillation. Journal of Agricultural and Food Chemistry, 55, 7847–7851.PubMedCrossRefGoogle Scholar
  14. Berger, R. G. (2007). Flavours and fragrances: Chemistry, bioprocessing and sustainability. Berlin: Springer Science & Business Media.CrossRefGoogle Scholar
  15. Berger, R. G., Krings, U., & Zorn, H. (2010). Biotechnological flavour generation, Food flavour technology (pp. 89–126). Chichester: Wiley-Blackwell.CrossRefGoogle Scholar
  16. Berliocchi, L., Ciociaro, A., Russo, R., Cassiano, M. G. V., Blandini, F., Rotiroti, D., Morrone, L. A., & Corasaniti, M. T. (2011). Toxic profile of bergamot essential oil on survival and proliferation of SH-SY5Y neuroblastoma cells. Food and Chemical Toxicology, 49, 2780–2792.PubMedCrossRefGoogle Scholar
  17. Boussaada, O., Skoula, M., Kokkalou, E., & Chemli, R. (2007). Chemical variability of flowers, leaves, and peels oils of four sour orange provenances. Journal of Essential Oil Bearing Plants, 10, 453–464.CrossRefGoogle Scholar
  18. Buettner, A., Mestres, M., Fischer, A., Guasch, J., & Schieberle, P. (2003). Evaluation of the most odour-active compounds in the peel oil of clementines (Citrus reticulata Blanco cv. clementine). European Food Research and Technology, 216, 11–14.CrossRefGoogle Scholar
  19. Carvalho-Freitas, M. I. R., & Costa, M. (2002). Anxiolytic and sedative effects of extracts and essential oil from Citrus aurantium L. Biological and Pharmaceutical Bulletin, 25, 1629–1633.PubMedCrossRefGoogle Scholar
  20. Castro-Luna, A., & Garcia-de-la-Guarda, R. (2016). Antifungal effect of Citrus paradisi “grapefruit” on strains of Candida albicans isolated from patients with denture stomatitis Diana Eugenia Churata-Oroya1, Donald Ramos-Perfecto2, Hilda Moromi-Nakata2, Elba Martínez-Cadillo2. Revista Estomatológica Herediana, 26, 78–84.CrossRefGoogle Scholar
  21. Ceccarelli, I., Masi, F., Fiorenzani, P., & Aloisi, A. M. (2002). Sex differences in the citrus lemon essential oil-induced increase of hippocampal acetylcholine release in rats exposed to a persistent painful stimulation. Neuroscience Letters, 330, 25–28.PubMedCrossRefGoogle Scholar
  22. Cheng, Y.-S., & Lee, C.-S. (1981). Composition of leaf essential oils from ten Citrus species. Proceedings of the National Science Council.Google Scholar
  23. Choi, H.-S., Song, H. S., Ukeda, H., & Sawamura, M. (2000). Radical-scavenging activities of citrus essential oils and their components: Detection using 1, 1-diphenyl-2-picrylhydrazyl. Journal of Agricultural and Food Chemistry, 48, 4156–4161.PubMedCrossRefGoogle Scholar
  24. Chutia, M., Bhuyan, P. D., Pathak, M., Sarma, T., & Boruah, P. (2009). Antifungal activity and chemical composition of Citrus reticulata Blanco essential oil against phytopathogens from North East India. LWT-Food Science and Technology, 42, 777–780.CrossRefGoogle Scholar
  25. Cirmi, S., Bisignano, C., Mandalari, G., & Navarra, M. (2016). Anti-infective potential of Citrus bergamia Risso et Poiteau (bergamot) derivatives: A systematic review. Phytotherapy Research, 30, 1404–1411.PubMedCrossRefGoogle Scholar
  26. Conde-Hernández, L. A., Espinosa-Victoria, J. R., Trejo, A., & Guerrero-Beltrán, J. Á. (2017). CO2-supercritical extraction, hydrodistillation and steam distillation of essential oil of rosemary (Rosmarinus officinalis). Journal of Food Engineering, 200, 81–86.CrossRefGoogle Scholar
  27. Costa, C. A., Cury, T. C., Cassettari, B. O., Takahira, R. K., Flório, J. C., & Costa, M. (2013). Citrus aurantium L. essential oil exhibits anxiolytic-like activity mediated by 5-HT 1A-receptors and reduces cholesterol after repeated oral treatment. BMC Complementary and Alternative Medicine, 13, 42.PubMedPubMedCentralCrossRefGoogle Scholar
  28. da Silva, J. K., da Trindade, R., Moreira, E. C., Maia, J. G. S., Dosoky, N. S., Miller, R. S., Cseke, L. J., & Setzer, W. N. (2017). Chemical diversity, biological activity, and genetic aspects of three Ocotea species from the Amazon. International Journal of Molecular Sciences, 18, 1081.PubMedCentralCrossRefPubMedGoogle Scholar
  29. Dahmoune, F., Nayak, B., Moussi, K., Remini, H., & Madani, K. (2015). Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves. Food Chemistry, 166, 585–595.PubMedCrossRefGoogle Scholar
  30. De Castro, M. L., Jimenez-Carmona, M., & Fernandez-Perez, V. (1999). Towards more rational techniques for the isolation of valuable essential oils from plants. TrAC Trends in Analytical Chemistry, 18, 708–716.CrossRefGoogle Scholar
  31. de Moraes Pultrini, A., Galindo, L. A., & Costa, M. (2006). Effects of the essential oil from Citrus aurantium L. in experimental anxiety models in mice. Life Sciences, 78, 1720–1725.CrossRefGoogle Scholar
  32. De Pasquale, F., Siragusa, M., Abbate, L., Tusa, N., De Pasquale, C., & Alonzo, G. (2006). Characterization of five sour orange clones through molecular markers and leaf essential oils analysis. Scientia Horticulturae, 109, 54–59.CrossRefGoogle Scholar
  33. Dosoky, N., & Setzer, W. (2018). Biological activities and safety of Citrus spp. essential oils. International Journal of Molecular Sciences, 19, 1966.PubMedCentralCrossRefPubMedGoogle Scholar
  34. Dosoky, N. S., Moriarity, D. M., & Setzer, W. N. (2016). Phytochemical and biological investigations of Conradina canescens. Natural Product Communications, 11, 25–28.PubMedGoogle Scholar
  35. Dugo, G., & Di Giacomo, A. (2002). The genus Citrus. London: Taylor & Francis Book Ltd.Google Scholar
  36. Dugo, G., Bonaccorsi, I., Sciarrone, D., Costa, R., Dugo, P., Mondello, L., Santi, L., & Fakhry, H. A. (2011). Characterization of oils from the fruits, leaves and flowers of the bitter orange tree. Journal of Essential Oil Research, 23, 45–59.CrossRefGoogle Scholar
  37. Ekundayo, O., Bakare, O., Adesomoju, A., & Stahl-Biskup, E. (1991). Composition of the leaf oil of grapefruit (Citrus paradisi MACF.). Journal of Essential Oil Research, 3, 55–56.CrossRefGoogle Scholar
  38. El-aal, H. A., & Halaweish, F. (2010). Food preservative activity of phenolic compounds in orange peel extracts (Citrus sinensis L.). Lucrări Ştiinţifice, 53, 233–240.Google Scholar
  39. Fantin, G., Fogagnolo, M., Maietti, S., & Rossetti, S. (2010). Selective removal of monoterpenes from bergamot oil by inclusion in deoxycholic acid. Journal of Agricultural and Food Chemistry, 58, 5438–5443.PubMedCrossRefPubMedCentralGoogle Scholar
  40. Faturi, C. B., Leite, J. R., Alves, P. B., Canton, A. C., & Teixeira-Silva, F. (2010). Anxiolytic-like effect of sweet orange aroma in Wistar rats. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 34, 605–609.PubMedCrossRefGoogle Scholar
  41. Ferhat, M. A., Meklati, B. Y., Smadja, J., & Chemat, F. (2006). An improved microwave Clevenger apparatus for distillation of essential oils from orange peel. Journal of Chromatography A, 1112, 121–126.PubMedCrossRefGoogle Scholar
  42. Ferhat, M. A., Meklati, B. Y., & Chemat, F. (2007). Comparison of different isolation methods of essential oil from Citrus fruits: cold pressing, hydrodistillation and microwave ‘dry’distillation. Flavour and Fragrance Journal, 22(6), 494–504.Google Scholar
  43. Fisher, K., & Phillips, C. A. (2006). The effect of lemon, orange and bergamot essential oils and their components on the survival of Campylobacter jejuni, Escherichia coli O157, Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus in vitro and in food systems. Journal of Applied Microbiology, 101, 1232–1240.PubMedCrossRefGoogle Scholar
  44. Friedman, M., Henika, P. R., Levin, C. E., & Mandrell, R. E. (2004). Antibacterial activities of plant essential oils and their components against Escherichia coli O157: H7 and Salmonella enterica in apple juice. Journal of Agricultural and Food Chemistry, 52, 6042–6048.PubMedCrossRefGoogle Scholar
  45. Gmitter, F. G., & Hu, X. (1990). The possible role of Yunnan, China, in the origin of contemporary Citrus species (Rutaceae). Economic Botany, 44, 267–277.CrossRefGoogle Scholar
  46. Goes, T. C., Antunes, F. D., Alves, P. B., & Teixeira-Silva, F. (2012). Effect of sweet orange aroma on experimental anxiety in humans. The Journal of Alternative and Complementary Medicine, 18, 798–804.PubMedCrossRefGoogle Scholar
  47. Gurib-Fakim, A., & Demarne, F. (1995). Aromatic plants of Mauritius: Volatile constituents of the leaf oils of Citrus aurantium L., Citrus paradisi Macfad and Citrus sinensis (L.) Osbeck. Journal of Essential Oil Research, 7, 105–109.CrossRefGoogle Scholar
  48. Hamdan, D. I., Abdulla, R. H., Mohamed, M. E., & El-Shazly, A. M. (2013). Chemical composition and biological activity of essential oils of Cleopatra mandarin (Citrus reshni) cultivated in Egypt. Journal of Pharmacognosy and Phytotherapy, 5, 83–90.Google Scholar
  49. Hata, T., Sakaguchi, I., Mori, M., Ikeda, N., Kato, Y., Minamino, M., & Watabe, K. (2003). Induction of apoptosis by Citrus paradisi essential oil in human leukemic (HL-60) cells. In Vivo (Athens, Greece), 17, 553–559.Google Scholar
  50. Hérent, M.-F., De Bie, V., & Tilquin, B. (2007). Determination of new retention indices for quick identification of essential oils compounds. Journal of Pharmaceutical and Biomedical Analysis, 43, 886–892.PubMedCrossRefGoogle Scholar
  51. Hodgson, R. W. (1967). Horticultural Varieties of Citrus. In W. Reuther, H. J. Webber & L. D. Batchelor (Eds.), The Citrus Industry, University of California, Berkeley, 431–459. Google Scholar
  52. Huang, W., & Leonas, K. K. (2000). Evaluating a one-bath process for imparting antimicrobial activity and repellency to nonwoven surgical gown fabrics. Textile Research Journal, 70, 774–782.CrossRefGoogle Scholar
  53. Igarashi, M., Ikei, H., Song, C., & Miyazaki, Y. (2014). Effects of olfactory stimulation with rose and orange oil on prefrontal cortex activity. Complementary Therapies in Medicine, 22, 1027–1031.PubMedCrossRefGoogle Scholar
  54. Ikeda, H., Takasu, S., & Murase, K. (2014). Contribution of anterior cingulate cortex and descending pain inhibitory system to analgesic effect of lemon odor in mice. Molecular Pain, 10, 14.PubMedPubMedCentralCrossRefGoogle Scholar
  55. Iturriaga, L., Olabarrieta, I., & de Marañón, I. M. (2012). Antimicrobial assays of natural extracts and their inhibitory effect against Listeria innocua and fish spoilage bacteria, after incorporation into biopolymer edible films. International Journal of Food Microbiology, 158, 58–64.PubMedCrossRefGoogle Scholar
  56. Jaeger, H., Roth, A., Toepfl, S., Holzhauser, T., Engel, K.-H., Knorr, D., Vogel, R. F., Bandick, N., Kulling, S., & Heinz, V. (2016). Opinion on the use of ohmic heating for the treatment of foods. Trends in Food Science and Technology, 55, 84–97.CrossRefGoogle Scholar
  57. Jia, H., Shao, T., Zhong, C., Li, H., Jiang, M., Zhou, H., & Wei, P. (2016). Evaluation of xylitol production using corncob hemicellulosic hydrolysate by combining tetrabutylammonium hydroxide extraction with dilute acid hydrolysis. Carbohydrate Polymers, 151, 676–683.PubMedCrossRefGoogle Scholar
  58. Kirbaslar, F. G., & Kirbaslar, S. I. (2003). Composition of cold-pressed bitter orange peel oil from Turkey. Journal of Essential Oil Research, 15, 6–9.CrossRefGoogle Scholar
  59. Kirbaşlar, F. G., Tavman, A., Dülger, B., & Türker, G. (2009). Antimicrobial activity of Turkish citrus peel oils. Pakistan Journal of Botany, 41, 3207–3212.Google Scholar
  60. Lauro, F., Ilari, S., Giancotti, L. A., Morabito, C., Malafoglia, V., Gliozzi, M., Palma, E., Salvemini, D., & Muscoli, C. (2016). The protective role of bergamot polyphenolic fraction on several animal models of pain. PharmaNutrition, 4, S35–S40.CrossRefGoogle Scholar
  61. Leggio, A., Leotta, V., Belsito, E. L., Di Gioia, M. L., Romio, E., Santoro, I., Taverna, D., Sindona, G., & Liguori, A. (2017). Aromatherapy: Composition of the gaseous phase at equilibrium with liquid bergamot essential oil. Chemistry Central Journal, 11, 111.PubMedPubMedCentralCrossRefGoogle Scholar
  62. Lehrner, J., Eckersberger, C., Walla, P., Pötsch, G., & Deecke, L. (2000). Ambient odor of orange in a dental office reduces anxiety and improves mood in female patients. Physiology & Behavior, 71, 83–86.CrossRefGoogle Scholar
  63. Lopes Campêlo, L. M., Moura Gonçalves, F. C., Feitosa, C. M., & de Freitas, R. M. (2011). Antioxidant activity of Citrus limon essential oil in mouse hippocampus. Pharmaceutical Biology, 49, 709–715.CrossRefGoogle Scholar
  64. Lota, M. L., de Rocca Serra, D., Jacquemond, C., Tomi, F., & Casanova, J. (2001). Chemical variability of peel and leaf essential oils of sour orange. Flavour and Fragrance Journal, 16, 89–96.CrossRefGoogle Scholar
  65. Lv, Y.-X., Zhao, S.-P., Zhang, J.-Y., Zhang, H., Xie, Z.-H., Cai, G.-M., & Jiang, W.-H. (2012). Effect of orange peel essential oil on oxidative stress in AOM animals. International Journal of Biological Macromolecules, 50, 1144–1150.PubMedCrossRefGoogle Scholar
  66. Ma, Q., Fan, X.-D., Liu, X.-C., Qiu, T.-Q., & Jiang, J.-G. (2015). Ultrasound-enhanced subcritical water extraction of essential oils from Kaempferia galangal L. and their comparative antioxidant activities. Separation and Purification Technology, 150, 73–79.CrossRefGoogle Scholar
  67. Matan, N., & Matan, N. (2008). Antifungal activities of anise oil, lime oil, and tangerine oil against molds on rubberwood (Hevea brasiliensis). International Biodeterioration & Biodegradation, 62, 75–78.CrossRefGoogle Scholar
  68. Matiz, G., Osorio, M. R., Camacho, F., Atencia, M., & Herazo, J. (2012). Effectiveness of antimicrobial formulations for acne based on orange (Citrus sinensis) and sweet basil (Ocimum basilicum L) essential oils. Biomédica, 32, 125–133.PubMedGoogle Scholar
  69. Minh Tu, N., Thanh, L., Une, A., Ukeda, H., & Sawamura, M. (2002). Volatile constituents of Vietnamese pummelo, orange, tangerine and lime peel oils. Flavour and Fragrance Journal, 17, 169–174.CrossRefGoogle Scholar
  70. Mitropoulou, G., Fitsiou, E., Spyridopoulou, K., Tiptiri-Kourpeti, A., Bardouki, H., Vamvakias, M., Panas, P., Chlichlia, K., Pappa, A., & Kourkoutas, Y. (2017). Citrus medica essential oil exhibits significant antimicrobial and antiproliferative activity. LWT-Food Science and Technology, 84, 344–352.CrossRefGoogle Scholar
  71. Mondello, L., Dugo, P., Bartle, K. D., Dugo, G., & Cotroneo, A. (1995). Automated HPLC-HRGC: A powerful method for essential oils analysis. Part V. Identification of terpene hydrocarbons of bergamot, lemon, mandarin, sweet orange, bitter orange, grapefruit, clementine and Mexican lime oils by coupled HPLC-HRGC-MS (ITD). Flavour and Fragrance Journal, 10, 33–42.CrossRefGoogle Scholar
  72. Moysan, A., Morlière, P., Averbeck, D., & Dubertret, L. (1993). Evaluation of phototoxic and photogenotoxic risk associated with the use of photosensitizers in suntan preparations: Application to tanning preparations containing bergamot oil. Skin Pharmacology and Physiology, 6, 282–291.CrossRefGoogle Scholar
  73. Murthy, K. N. C., Jayaprakasha, G. K., & Patil, B. S. (2012). D-limonene rich volatile oil from blood oranges inhibits angiogenesis, metastasis and cell death in human colon cancer cells. Life Sciences, 91, 429–439.CrossRefGoogle Scholar
  74. Nagai, K., Niijima, A., Horii, Y., Shen, J., & Tanida, M. (2014). Olfactory stimulatory with grapefruit and lavender oils change autonomic nerve activity and physiological function. Autonomic Neuroscience, 185, 29–35.PubMedCrossRefGoogle Scholar
  75. Nagy, M. M., Al-Mahdy, D. A., Abd El Aziz, O. M., Kandil, A. M., Tantawy, M. A., & El Alfy, T. S. (2018). Chemical composition and antiviral activity of essential oils from Citrus reshni hort. ex Tanaka (Cleopatra mandarin) cultivated in Egypt. Journal of Essential Oil Bearing Plants, 21, 264–272.CrossRefGoogle Scholar
  76. Namazi, M., Akbari, S. A. A., Mojab, F., Talebi, A., Majd, H. A., & Jannesari, S. (2014). Effects of citrus aurantium (bitter orange) on the severity of first-stage labor pain. Iranian journal of Pharmaceutical Research: IJPR, 13, 1011.PubMedGoogle Scholar
  77. Navarra, M., Ferlazzo, N., Cirmi, S., Trapasso, E., Bramanti, P., Lombardo, G. E., Minciullo, P. L., Calapai, G., & Gangemi, S. (2015). Effects of bergamot essential oil and its extractive fractions on SH-SY5Y human neuroblastoma cell growth. Journal of Pharmacy and Pharmacology, 67, 1042–1053.PubMedCrossRefGoogle Scholar
  78. Niijima, A., & Nagai, K. (2003). Effect of olfactory stimulation with flavor of grapefruit oil and lemon oil on the activity of sympathetic branch in the white adipose tissue of the epididymis. Experimental Biology and Medicine, 228, 1190–1192.PubMedCrossRefGoogle Scholar
  79. Njoroge, S. M., Koaze, H., Karanja, P. N., & Sawamura, M. (2005). Essential oil constituents of three varieties of Kenyan sweet oranges (Citrus sinensis). Flavour and Fragrance Journal, 20, 80–85.CrossRefGoogle Scholar
  80. Njoroge, S. M., Phi, N. T. L., & Sawamura, M. (2009). Chemical composition of peel essential oils of sweet oranges (Citrus sinensis) from Uganda and Rwanda. Journal of Essential Oil Bearing Plants, 12, 26–33.CrossRefGoogle Scholar
  81. Ogeturk, M., Kose, E., Sarsilmaz, M., Akpinar, B., Kus, I., & Meydan, S. (2010). Effects of lemon essential oil aroma on the learning behaviors of rats. Neurosciences, 15, 292–293.PubMedGoogle Scholar
  82. Okunowo, W. O., Oyedeji, O., Afolabi, L. O., & Matanmi, E. (2013). Essential oil of grape fruit (Citrus paradisi) peels and its antimicrobial activities. American Journal of Plant Sciences, 4, 1.CrossRefGoogle Scholar
  83. Oliveira, S. A. C., Zambrana, J. R. M., Di Iorio, F. B. R., Pereira, C. A., & Jorge, A. O. C. (2014). The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms. Brazilian Oral Research, 28, 22–27.PubMedCrossRefGoogle Scholar
  84. Oshaghi, M., Ghalandari, R., Vatandoost, H., Shayeghi, M., Kamali-Nejad, M., Tourabi-Khaledi, H., Abolhassani, M., & Hashemzadeh, M. (2003). Repellent effect of extracts and essential oils of Citrus limon (Rutaceae) and Melissa officinalis (Labiatae) against main malaria vector, Anopheles stephensi (Diptera: Culicidae). Iranian Journal of Public Health, 32, 47–52.Google Scholar
  85. Pimenta, F. C. F., Alves, M. F., Pimenta, M. B. F., Melo, S. A. L., Almeida, A. A. F. D., Leite, J. R., Pordeus, L. C. D. M., Diniz, M. D. F. F. M., & Almeida, R. N. D. (2016). Anxiolytic effect of Citrus aurantium L. on patients with chronic myeloid leukemia. Phytotherapy Research, 30, 613–617.PubMedCrossRefGoogle Scholar
  86. Pollien, P., Ott, A., Fay, L., Maignial, L., & Chaintreau, A. (1998). Simultaneous distillation-extraction: Preparative recovery of volatiles under mild conditions in batch or continuous operations. Flavour and Fragrance Journal, 13, 413–423.CrossRefGoogle Scholar
  87. Qiao, Y., Xie, B. J., Zhang, Y., Zhang, Y., Fan, G., Yao, X. L., & Pan, S. Y. (2008). Characterization of aroma active compounds in fruit juice and peel oil of Jinchen sweet orange fruit (Citrus sinensis (L.) Osbeck) by GC-MS and GC-O. Molecules, 13, 1333–1344.PubMedPubMedCentralCrossRefGoogle Scholar
  88. Ramadan, W., Mourad, B., Ibrahim, S., & Sonbol, F. (1996). Oil of bitter orange: New topical antifungal agent. International Journal of Dermatology, 35, 448–449.PubMedCrossRefGoogle Scholar
  89. Rimini, S., Petracci, M., & Smith, D. P. (2014). The use of thyme and orange essential oils blend to improve quality traits of marinated chicken meat. Poultry Science, 93, 2096–2102.PubMedCrossRefGoogle Scholar
  90. Rombolà, L., Amantea, D., Russo, R., Adornetto, A., Berliocchi, L., Tridico, L., Corasaniti, M., Sakurada, S., Sakurada, T., & Bagetta, G. (2016). Rational basis for the use of bergamot essential oil in complementary medicine to treat chronic pain. Mini Reviews in Medicinal Chemistry, 16, 721–728.PubMedCrossRefGoogle Scholar
  91. Rotiroti, D., & Bagetta, G. (2009). Effects of systemic administration of the essential oil of bergamot (BEO) on gross behaviour and EEG power spectra recorded from the rat hippocampus and cerebral cortex. Functional Neurology, 24, 107.PubMedGoogle Scholar
  92. Rubio-Rodríguez, N., Sara, M., Beltrán, S., Jaime, I., Sanz, M. T., & Rovira, J. (2008). Supercritical fluid extraction of the omega-3 rich oil contained in hake (Merluccius capensisMerluccius paradoxus) by-products: Study of the influence of process parameters on the extraction yield and oil quality. The Journal of Supercritical Fluids, 47, 215–226.CrossRefGoogle Scholar
  93. Russo, R., Cassiano, M. G. V., Ciociaro, A., Adornetto, A., Varano, G. P., Chiappini, C., Berliocchi, L., Tassorelli, C., Bagetta, G., & Corasaniti, M. T. (2014). Role of D-limonene in autophagy induced by bergamot essential oil in SH-SY5Y neuroblastoma cells. PLoS One, 9, e113682.PubMedPubMedCentralCrossRefGoogle Scholar
  94. Safajou, F., Shahnazi, M., & Nazemiyeh, H. (2014). The effect of lemon inhalation aromatherapy on nausea and vomiting of pregnancy: A double-blinded, randomized, controlled clinical trial. Iranian Red Crescent Medical Journal, 16, e14360.PubMedPubMedCentralGoogle Scholar
  95. Saiyudthong, S., & Marsden, C. A. (2011). Acute effects of bergamot oil on anxiety-related behaviour and corticosterone level in rats. Phytotherapy Research, 25, 858–862.PubMedCrossRefGoogle Scholar
  96. Salengke, S., Sastry, S., & Zhang, H. (2012). Pulsed electric field technology: Modeling of electric field and temperature distributions within continuous flow PEF treatment chamber. International Food Research Journal, 19, 1255.Google Scholar
  97. Sawamura, M. (2011). Citrus essential oils: Flavor and fragrance. Somerset: Wiley.Google Scholar
  98. Sawamura, M., Tu, N. T. M., Yu, X., & Xu, B. (2005). Volatile constituents of the peel oils of several sweet oranges in China. Journal of Essential Oil Research, 17, 2–6.CrossRefGoogle Scholar
  99. Sensoy, I., & Sastry, S. (2004). Extraction using moderate electric fields. Journal of Food Science, 69, FEP7–FEP13.CrossRefGoogle Scholar
  100. Setzer, W. N. (2009). Essential oils and anxiolytic aromatherapy. Natural Product Communications, 4, 1305–1316.PubMedCrossRefGoogle Scholar
  101. Sharifi-Rad, J., Sureda, A., Tenore, G. C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., Sharifi-Rad, M., Loizzo, M. R., & Ademiluyi, A. O. (2017). Biological activities of essential oils: From plant chemoecology to traditional healing systems. Molecules, 22, 70.PubMedCentralCrossRefPubMedGoogle Scholar
  102. Sharma, N., & Tripathi, A. (2006). Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens. World Journal of Microbiology and Biotechnology, 22, 587–593.CrossRefGoogle Scholar
  103. Shaw, P. E. (1979). Review of quantitative analyses of citrus essential oils. Journal of Agricultural and Food Chemistry, 27, 246–257.CrossRefGoogle Scholar
  104. Smith, D. C., Forland, S., Bachanos, E., Matejka, M., & Barrett, V. (2001). Qualitative analysis of citrus fruit extracts by GC/MS: An undergraduate experiment. The Chemical Educator, 6, 28–31.CrossRefGoogle Scholar
  105. Snow, N. H., & Slack, G. C. (2002). Head-space analysis in modern gas chromatography. TrAC Trends in Analytical Chemistry, 21, 608–617.CrossRefGoogle Scholar
  106. Stiles, K. (2017). The essential oils complete reference guide: Over 250 recipes for natural wholesome aromatherapy. Salem, MA: Page Street Publishing.Google Scholar
  107. Sun, Y., Chen, S., Zhang, C., Liu, Y., Ma, L., & Zhang, X. (2018). Effects of sub-minimum inhibitory concentrations of lemon essential oil on the acid tolerance and biofilm formation of Streptococcus mutans. Archives of Oral Biology, 87, 235241.PubMedCrossRefGoogle Scholar
  108. Swamy, M. K., Akhtar, M. S., & Sinniah, U. R. (2016). Antimicrobial properties of plant essential oils against human pathogens and their mode of action: An updated review. Evidence-Based Complementary and Alternative Medicine, 2016, 3012462.PubMedPubMedCentralCrossRefGoogle Scholar
  109. Swingle, W. T. (1967). The botany of citrus and its wild relatives. In W. Reuther, H. J. Webber & L. D. Batchelor (Eds.), The Citrus Industry, University of California, Berkeley, pp. 190–430.Google Scholar
  110. Tao, N., Jia, L., & Zhou, H. (2014). Anti-fungal activity of Citrus reticulata Blanco essential oil against Penicillium italicum and Penicillium digitatum. Food Chemistry, 153, 265–271.PubMedCrossRefGoogle Scholar
  111. Tisserand, R., & Young, R. (2013). Essential oil safety-e-book: A guide for health care professionals. Elsevier Health Sciences, Churchill Livingstone (pp. 784).Google Scholar
  112. Tranchida, P. Q., Bonaccorsi, I., Dugo, P., Mondello, L., & Dugo, G. (2012). Analysis of Citrus essential oils: State of the art and future perspectives. A review. Flavour and Fragrance Journal, 27, 98–123.CrossRefGoogle Scholar
  113. Tundis, R., Loizzo, M. R., Bonesi, M., Menichini, F., Mastellone, V., Colica, C., & Menichini, F. (2012). Comparative study on the antioxidant capacity and cholinesterase inhibitory activity of Citrus aurantifolia Swingle, C. aurantium L., and C. bergamia Risso and Poit. peel essential oils. Journal of Food Science, 77, H40–H46.PubMedCrossRefGoogle Scholar
  114. Ullah, N., Khan, M. A., Khan, T., & Ahmad, W. (2014). Nephroprotective potentials of Citrus Aurantium: A prospective pharmacological study on experimental models. Pakistan Journal of Pharmaceutical Sciences, 27, 505–510.PubMedGoogle Scholar
  115. Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., & Pérez-Álvarez, J. (2008a). Antifungal activity of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) essential oils. Food Control, 19, 1130–1138.CrossRefGoogle Scholar
  116. Viuda-Martos, M., Ruiz-Navajas, Y., Fernández-López, J., & Perez-Álvarez, J. (2008b). Antibacterial activity of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) essential oils. Journal of Food Safety, 28, 567–576.CrossRefGoogle Scholar
  117. Viuda-Martos, M., Mohamady, M., Fernández-López, J., ElRazik, K. A., Omer, E., Pérez-Alvarez, J., & Sendra, E. (2011). In vitro antioxidant and antibacterial activities of essentials oils obtained from Egyptian aromatic plants. Food Control, 22, 1715–1722.CrossRefGoogle Scholar
  118. Vorobiev, E., & Lebovka, N. (2010). Enhanced extraction from solid foods and biosuspensions by pulsed electrical energy. Food Engineering Reviews, 2, 95–108.CrossRefGoogle Scholar
  119. Wu, T., Cheng, D., He, M., Pan, S., Yao, X., & Xu, X. (2014). Antifungal action and inhibitory mechanism of polymethoxylated flavones from Citrus reticulata Blanco peel against Aspergillus niger. Food Control, 35, 354–359.CrossRefGoogle Scholar
  120. Xiao, Z., Ma, S., Niu, Y., Chen, F., & Yu, D. (2016). Characterization of odour-active compounds of sweet orange essential oils of different regions by gas chromatography-mass spectrometry, gas chromatography-olfactometry and their correlation with sensory attributes. Flavour and Fragrance Journal, 31, 41–50.CrossRefGoogle Scholar
  121. Xiong, Y., Zhao, Z., Zhu, L., Chen, Y., Ji, H., & Yang, D. (2013). Removal of three kinds of phthalates from sweet orange oil by molecular distillation. LWT-Food Science and Technology, 53, 487–491.CrossRefGoogle Scholar
  122. Yi, L.-T., Xu, H.-L., Feng, J., Zhan, X., Zhou, L.-P., & Cui, C.-C. (2011). Involvement of monoaminergic systems in the antidepressant-like effect of nobiletin. Physiology & Behavior, 102, 1–6.CrossRefGoogle Scholar
  123. Yi, F., Jin, R., Sun, J., Ma, B., & Bao, X. (2018). Evaluation of mechanical-pressed essential oil from Nanfeng mandarin (Citrus reticulata Blanco cv. Kinokuni) as a food preservative based on antimicrobial and antioxidant activities. LWT-Food Science and Technology, 95, 346–353.CrossRefGoogle Scholar
  124. Yip, Y. B., & Tam, A. C. Y. (2008). An experimental study on the effectiveness of massage with aromatic ginger and orange essential oil for moderate-to-severe knee pain among the elderly in Hong Kong. Complementary Therapies in Medicine, 16, 131–138.PubMedCrossRefGoogle Scholar
  125. Yu, L., Yan, J., & Sun, Z. (2017). D-limonene exhibits anti-inflammatory and antioxidant properties in an ulcerative colitis rat model via regulation of iNOS, COX-2, PGE2 and ERK signaling pathways. Molecular Medicine Reports, 15, 2339–2346.PubMedCrossRefGoogle Scholar
  126. Zagklis, D. P., & Paraskeva, C. A. (2015). Purification of grape marc phenolic compounds through solvent extraction, membrane filtration and resin adsorption/desorption. Separation and Purification Technology, 156, 328–335.CrossRefGoogle Scholar
  127. Zarrad, K., Hamouda, A. B., Chaieb, I., Laarif, A., & Jemâa, J. M.-B. (2015). Chemical composition, fumigant and anti-acetylcholinesterase activity of the Tunisian Citrus aurantium L. essential oils. Industrial Crops and Products, 76, 121–127.CrossRefGoogle Scholar
  128. Zhou, X., Zhao, Y., He, C. C., & Li, J. (2012). Preventive effects of Citrus reticulata essential oil on bleomycin-induced pulmonary fibrosis in rats and the mechanism. Zhong Xi Yi Jie He Xue Bao. Journal of Chinese Integrative Medicine, 10, 200–209.PubMedCrossRefGoogle Scholar
  129. Zu, Y., Yu, H., Liang, L., Fu, Y., Efferth, T., Liu, X., & Wu, N. (2010). Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules, 15, 3200–3210.PubMedPubMedCentralCrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Ali Osman
    • 1
  1. 1.Agricultural Biochemistry Department, Faculty of AgricultureZagazig UniversityZagazigEgypt

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