Sample Preparation Focusing on Plant Omics

  • Rodrigo Moretto Galazzi
  • Jemmyson Romário de Jesus
  • Marco Aurélio Zezzi ArrudaEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1073)


Because of strong impact of omics in many fields, and the complexity of the samples when focusing on areas such as genomics, (metallo)proteomics, metabolomics, among others, it is easy to rationalize the great importance that sample preparation has for achieving reliable results, mainly considering plant science. Then, this chapter points out applications of the sample preparation focusing on such areas, and a diversity of strategies, techniques, and procedures is highlighted and commented.


Proteomics Metallomics Ionomics Metabolomics Extraction Decomposition 



Ammonium acetate/methanol


Adenosine diphosphate


Adenosine triphosphate


Banded iron formation


Capillary electrophoresis-inductively coupled plasma-mass spectrometry


Deoxyribonucleic acid


Electrospray ionization mass spectrometry


Electrospray ionization-high-field asymmetric waveform ion mobility spectrometry-ion trap mass spectrometry


Fluorescence resonance energy transfer


Fourier transform ion cyclotron resonance mass spectrometry


High-performance liquid chromatography with diode array detector


High-performance liquid chromatography with diode array detector coupled to electrospray ionization tandem mass spectrometry


High-performance liquid chromatography with ultraviolet detector


Heat reflux extraction


Isotope-coded affinity


Inductively coupled plasma mass spectrometry


Isoelectric focusing


Isobaric tags for relative and absolute quantification


Jasmonic acid


Liquid chromatography


Liquid chromatography coupled to electrospray ionization tandem mass spectrometry


Liquid chromatography-inductively coupled plasma mass spectrometry


Liquid chromatography tandem-mass spectrometry


Liquid chromatography mass spectrometry


Microwave-assisted extraction


Matrix-assisted laser desorption/ionization coupled mass spectrometry




Mass spectrometry


Methyl tert-butyl ether


Multidimensional protein identification technology


Nicotinamide adenine dinucleotide phosphate


Nano-electrospray ionization quadrupole time-of-flight


Nanoscale secondary ion mass spectrometry


Nuclear magnetic resonance


PEI-assisted RuBisCO cleanup




Polyethylene glycol


Point isoelectric


Sodium phosphate/methanol


Pathogenesis related


Posttranslational modifications


Reversed-phase chromatography


Reversed-phase high-performance liquid chromatography-ultraviolet-electrospray ionization mass spectrometry


Ribulose-1,5-bisphosphate carboxylase/oxygenase


Size exclusion chromatography-ultraviolet


Sodium dodecyl sulfate polyacrylamide gel electrophoresis


Tandem affinity purification mass spectrometry


Trichloroacetic acid


Ultrasound-assisted extraction


Ultrahigh-performance liquid chromatography with diode array detector coupled to electrospray ionization tandem mass spectrometry


Ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry





The authors gratefully acknowledge the financial support of Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Financiadora de Estudos e Projetos (FINEP).


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Rodrigo Moretto Galazzi
    • 1
  • Jemmyson Romário de Jesus
    • 1
  • Marco Aurélio Zezzi Arruda
    • 1
    Email author
  1. 1.Universidade Estadual de Campinas – UnicampCampinasBrazil

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