Study of Growth and Production of Botrytis Cinerea Conidia of Some Morrocan Isolates in Different Nutrients Media

  • Ahlem HamdacheEmail author
  • Mohammed Ezziyyani
  • Ahmed Lamarti
Conference paper
Part of the Advances in Intelligent Systems and Computing book series (AISC, volume 911)


Botrytis species include some serious fungal plant pathogens, which are implicated in many diseases affecting flowers, fruits, cereals, legumes, and other vegetables. In particular, Botrytis cinerea attacks economically important crops such as carrots, grapes, lettuce, strawberries, and tobacco, producing various leaf spot diseases and grey mould. In this work, we have studied, in vitro, the growth and the sporulation of some isolates of Botrytis cinerea in different nutrient media. Our aim was to select the isolate the most pathogen to use it in biological control tests. For this purpose, ten isolates of Botrytis cinerea were isolated, purified and identified in the laboratory. Seven isolates were isolated from samples of strawberry fruit harvested from fields; four of them, are originated from strawberry fields of Zlaoula and Laâwamra (Larache) and three are originated from fields of Moulay Bouselham (Algharb). Others are isolated from postharvest strawberry. The growth and sporulation of all of the isolates are studied and compared in different nutrient media PDA, MEA, Czapek and organic medium of strawberry. Among all of the isolates tested, Botrytis cinerea Bt7 originated from fields of Zlaoula was the most important isolate with maximum growth in all of nutrients media, and maximum sporulation (74,7.105 sp/ml). B. cinerea was inoculated artificially in leaf and fruit stawberry and it causes the visible disease symptoms of grey mould.


Botrytis cinerea Nutrient media Mycelial growth Sporulation Pathogenicity 


  1. 1.
    Jarvis, W.R.: Botryotinia and Botrytis species; taxonomy, physiology and pathogenicity. Monograph No. 15, Canadian Department of Agriculture, Ottawa, p. 195 (1977)Google Scholar
  2. 2.
    Fournier, E., Gladieux, P., Giraud, T.: The “Dr Jekyll and Mr Hyde fungus”: noble rot versus gray mold symptoms of Botrytis cinerea on grapes. Evol. Appl. 6, 960–969 (2013)CrossRefGoogle Scholar
  3. 3.
    Martinez, F., Dubos, B., Fermaud, M.: The role of saprotrophy and virulence in the population dynamics of botrytis cinerea in vineyards. Phytopathology 95, 692–700 (2005)CrossRefGoogle Scholar
  4. 4.
    Pathirana, R., Cheah, L.H., Carimi, F., Carra, A.: Low temperature stored in cryobank® maintains pathogenicity in grapevine. Cryoletters 30, 84 (2009)Google Scholar
  5. 5.
    Butt, T.C., Wang, S.F., Hall, R.: Degeneration of entomogenous fungi. In: Eilenberg, J., Hokkanen, H. (eds.) An Ecological and Societal Approach to Biological Control, pp. 213–226. Springer, Dordrecht (2006)Google Scholar
  6. 6.
    Moore, E.: Fundamentals of the Fungi. Prentice Hall, New Jersey, p. 574 (1996)Google Scholar
  7. 7.
    Pérez, L., Ramírez, C.: Effect of the variables, conditions of fermentation and the substrate in the production of Trichoderma harzianum. Ph.D. dissertation, Pontificia Universidad Javeriana, Bogotá, Colombia, p. 153 (2000)Google Scholar
  8. 8.
    Griffin, D.H.: Fungal Physiology, pp. 260–279. John Wiley & Sons, Inc., New Jersey (1996)Google Scholar
  9. 9.
    Hegedus, D., Bidochka, M., Khachatourians, G.: Beauveria bassiana submerged conidia production in a defined medium containing chitin, two hexosamines or glucose. Appl. Microbiol. Biotechnol. 33, 641–647 (1990)CrossRefGoogle Scholar
  10. 10.
    Bidochka, M., Low, N., Khachatourians, G.: Carbohydrate storage in the entomopathogenic fungus Beauveria bassiana. Appl. Environ. Microbiol. 56, 3186–3190 (1990)Google Scholar
  11. 11.
    Kamp, A.M., Bidochka, M.J.: Conidium production by insect pathogenic fungi on commercially available agars. Lett. Appl. Microbiol. 35, 74–77 (2002)CrossRefGoogle Scholar
  12. 12.
    Samson, R.A., Hoekstra, E.S., C.A.N.V. Oorschot: Introduction to food-borne fungi. 2nd ed. Centraalbureau Voor Schimmelcultures, BAARN. Institute of the Royal Netherlands, Academy of Arts and Sciences, p. 248 (1984)Google Scholar
  13. 13.
    Botton, B., Breton, A., Fevre, M., Gauthier, S., Guy, P., Larpent, J.P., Reymond, P., Sanglier, J.J., Vayssier, Y., Veau, P.: Moisissures utiles et nuisibles: importance industrielle. 2e éd. rev. et compl. Paris, Milan, Barcelone, Masson, p. 512 (1990)Google Scholar
  14. 14.
    Tiedemann, A.V.: Evidence for a primary role of active oxygen species in induction of host cell death during infection of bean leaves with Botrytis cinerea. Physiol. Mol. Plant Pathol. 50(3), 151–166 (1997)CrossRefGoogle Scholar
  15. 15.
    Decognet, V., Bardin, M., Trottin-Caudal, Y., Nicot, P.C.: Rapid change in the genetic diversity of Botrytis cinerea populations after the introduction of strains in a tomato glasshouse. Phytopathology 99, 185–193 (2009)CrossRefGoogle Scholar
  16. 16.
    Mirzaei, S., Mohammadi-Goltapeh, E., Shams-Bakhsh, M., Safaie, N., Chaichi, M.: Genetic and phenotypic diversity among Botrytis cinerea isolates in Iran. J. Phytopathol. 157, 474–482 (2009)CrossRefGoogle Scholar
  17. 17.
    Kosuge, T., Hewitt, W.B.: Exudates of grape berries and their effect on germination of conidia of Botrytis cinerea. Phytopathology 54, 167–172 (1964)Google Scholar
  18. 18.
    Yoder, O.C., Whalen, M.L.: Factors affecting postharvest infection of stored cabbage tissue by Botrytis cinerea. Can. J. Bot. 53, 691–699 (1975)CrossRefGoogle Scholar
  19. 19.
    Li, G.Q., Huang, H.C., Acharya, S.N., Erickson, R.S.: Biological control of blossom blight of alfalfa caused by Botrytis cinerea under environmentally controlled and field conditions. Plant Dis. 88, 1246–1251 (2004)CrossRefGoogle Scholar
  20. 20.
    Edwards, S.G., Seddon, B.: Mode of antagonism of Brevibacillus brevis against Botrytis cinerea in vitro. J. Appl. Microbiol. 91, 652–659 (2001)CrossRefGoogle Scholar
  21. 21.
    Hjeljord, L.G., Stensvand, A., Tronsmo, A.: Antagonism of nutrient-activated conidia of Trichoderma harzianum (atroviride) P1 against Botrytis cinerea. Phytopathology 91(12), 1172–1180 (2001)CrossRefGoogle Scholar
  22. 22.
    Guetsky, R., Shtienberg, D., Elad, Y., Dinoor, A.: Combining biocontrol agents to reduce the variability of biological control. Phytopathology 91(7), 621–627 (2001)CrossRefGoogle Scholar
  23. 23.
    Buck, J.W.: In vitro antagonism of Botrytis cinerea by phylloplane yeasts. Can. J. Bot. 80(8), 885–891 (2002)CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Ahlem Hamdache
    • 1
    Email author
  • Mohammed Ezziyyani
    • 2
  • Ahmed Lamarti
    • 1
  1. 1.Faculty of Sciences of Tetouan, Department of BiologyAbdelmalek Essaâdi UniversityTétouanMorocco
  2. 2.Polydisciplinary Faculty of Larache, Department of Life SciencesAbdelmalek Essaâdi UniversityLaracheMorocco

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