Separation and Purification of Uncharged Molecules

  • Abhijit Mondal
  • Ria Majumdar
  • Nibedita Mahata
  • Biswanath BhuniaEmail author


The purification and separation of uncharged complexes is a challenging issue in the field of biotechnology. The most common metals required for our biological system are iron and cobalt, which play an important role in all living systems as these coordinate with haemoglobin and vitamin B12, respectively. Therefore, careful measurement of vitamin B12 and haemoglobin is a prerequisite for early detection of several diseases. Since detection of these compounds usually carried out in blood samples, therefore, purification of these compounds is utmost important before their measurement. Furthermore, the separation of uncharged dyes from wastewater sources is important to protect human health through preservation of supply of water which will protect the spread of various life-threatening diseases. Although there are several unit operations applied for the separation of uncharged complexes, however, all operations have their own drawbacks. The aim of this book chapter is the systematic and comparative presentation of the available information on the separation of these compounds owing to their application in various fields of biotechnology.


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Abhijit Mondal
    • 1
  • Ria Majumdar
    • 2
  • Nibedita Mahata
    • 3
  • Biswanath Bhunia
    • 4
    Email author
  1. 1.Department of Chemical EngineeringNational Institute of Technology AgartalaBarjala, JiraniaIndia
  2. 2.Department of Civil EngineeringNational Institute of Technology AgartalaBarjala, JiraniaIndia
  3. 3.Department of BioTechnologyNational Institute of Technology DurgapurDurgapurIndia
  4. 4.Department of Bio EngineeringNational Institute of Technology AgartalaBarjala, JiraniaIndia

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