Quantification of Root Anatomical Traits in RGP Transgenic Maize Plants Based on Micro-CT
The RGP transgenic plants show good resistance to drought especially reflected in corn yield. However, little is known about root traits which contribute to drought resistance. Here, we characterized root anatomy in the transgenic plants based on micro-CT scanning. Quantitative analysis of root anatomical traits showed that the drought-resistant RGP transgenic plants had larger root and stele cross-sectional areas in the fourth and the fifth whorls of nodal roots. The metaxylem vessel number and total area of metaxylem vessels was higher or larger in the fifth and the sixth whorls of nodal roots from RGP transgenic plants.
KeywordsMaize roots Anatomical traits Drought Micro-CT
This work was supported by Beijing Natural Science Foundation (5174033), Beijing Postdoctoral Research Foundation (2016 ZZ-66), Postdoctoral Research Foundation of Beijing Academy of Agriculture and Forestry Sciences (Postdoctoral Number: 157939).
- 1.Heisey, P.W., Morris, M.L.: Economic impact of water-limited conditions on cereal grain production. In: Ribaut, J.M. (ed.) Drought Adaptation in Cereals. Haworth Press, New York (2006)Google Scholar
- 10.Pan, X., Ma, L., Zhang, Y., Wang, J., Du, J., Guo, X.: Three-dimensional reconstruction of maize roots and quantitative analysis of metaxylem vessels based on X-ray micro-computed tomography. Can. J. Plant Sci. 98, 457–466 (2018)Google Scholar
- 13.Dorca-Fornell, C., Pajor, R., Lehmeier, C., Pérez-Bueno, M., Bauch, M., Sloan, J., et al.: Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern. Plant J. 76, 914–929 (2013)CrossRefGoogle Scholar