Molecular Genetics and Genomics of Fungal Infections

  • Shivaprakash M. Rudramurthy
  • Arunaloke Chakrabarti


Fungal infections of central nervous system (CNS) are increasingly encountered in various clinical settings. Any pathogenic fungus is able to produce CNS infection, though only certain groups are neurotropic. The outcome of CNS fungal infections depends on early diagnosis and prompt management. But, the early diagnosis faces stiff challenges due to general considerations of rarity of CNS fungal infection, non-specific clinical symptoms and signs and difficulty in collection of samples for conventional diagnosis. Application of genomic-based molecular techniques is usually rapid and sensitive and may detect the causative agent accurately and help appropriate and prompt antifungal therapy. These techniques can be applied either for detection of nucleic acid in clinical samples or for the accurate identification of fungi isolated from the clinical specimen. Detection of nucleic acid in clinical sample faces the challenge of extraction of nucleic acid, inadequate amount nucleic acid in sample and contamination during test procedure. The current chapter describes the existing knowledge on genomic-based molecular tests for diagnosis and monitoring of CNS fungal diseases.


Central nervous system Diagnostic procedures DNA extraction Fungal infections Genomics Molecular diagnosis Molecular genetics Molecular test formats 



Acquired immunodeficiency syndrome


Centers for Disease Control and Prevention


Cryptococcal meningitis


Central nervous system


Cerebrospinal fluid


Computed tomography


Elongation factor


Human immunodeficiency virus


Hyperbranched rolling circle amplification


International Society for Human and Animal Mycology


Internal transcribed spacer


Loop-mediated isothermal amplification


Lateral flow assay




Minimal inhibitory concentration


Magnetic resonance imaging


Polymerase chain reaction


Phospholipase B


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Shivaprakash M. Rudramurthy
    • 1
  • Arunaloke Chakrabarti
    • 1
    • 2
  1. 1.Department of Medical MicrobiologyPostgraduate Institute of Medical Education and ResearchChandigarhIndia
  2. 2.Center of Advance Research in Medical Mycology, WHO Collaborating Center for Reference and Research of Fungi of Medical ImportanceNational Culture Collection of Pathogenic FungiChandigarhIndia

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