Separation and Purification of Vitamins: Vitamins B1, B2, B6, C and K1

  • P. Senthil KumarEmail author
  • G. Janet Joshiba


Vitamins are substances that are required by the human body for enhanced growth and development. Altogether thirteen types of vitamins are essential for the normal functioning of the body. The vitamins are broadly classified as water-soluble and fat-soluble vitamins which are further distinguished as vitamin A, B, C, E and K. The water-soluble and fat-soluble vitamins are separated and purified using various techniques such as capillary electrophoresis, HPLC and chromatography. Ion-exchange chromatography is being the most popular. It plays a major role in various environmental applications such as ecological remediation, water softening, catalysis, hydrometallurgy, wastewater treatment and biomolecular partitions. Various synthetic resins and several other resins have been used for the separation and purification of the vitamins. An overview of the different methodologies involved in the process of ion-exchange chromatography used in the separation and purification of vitamins B1, B2, B6, C and K1 is presented in this chapter.


Vitamins Ion-exchange chromatography Covalent Separation and purification 


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Chemical EngineeringSSN College of EngineeringChennaiIndia

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