Separation and Purification of Nucleotides, Nucleosides, Purine and Pyrimidine Bases by Ion Exchange

  • P. Senthil KumarEmail author
  • P. R. Yaashikaa


The examination of a whole arrangement of constituents of biological significance, the nucleic acids, nucleotides, and related substances, has been blocked extensively by the absence of particular strategies for the separation and characterization of their nitrogenous constituents. The strategies accessible for the separation and purification of nucleotides and nucleic acids have been connected to an assortment of materials. Despite the fact that refinement of the nucleotides was endeavored by different systems, for example, precipitation of the nucleotides as insoluble salts and ion-exchange procedures, an appropriate general strategy is still to be developed. The detachment into singular nucleotides by ion-exchange chromatography has been the main dependable method for assessing the nucleotide content. Combination of purines, pyrimidines, amino acids, and different nitrogenous substances was observed to be isolated by ion chromatography on a solitary column of the cation-exchange resin and Dowex 50. The mixture components were eluted with hydrochloric acid and controlled by either their response with ninhydrin or UV adsorption. Ion exchange has turned out to be one of the real techniques for fractionation of organic substances.


Ion-exchange chromatography Nucleotides Nucleosides Purines and pyrimidines Purification Applications 


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Authors and Affiliations

  1. 1.Department of Chemical EngineeringSSN College of EngineeringChennaiIndia

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