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Separation and Purification of Amino Acids

  • Khush Bakhat Alia
  • Habibullah NadeemEmail author
  • Ijaz Rasul
  • Farrukh Azeem
  • Sabir Hussain
  • Muhammad Hussnain Siddique
  • Saima Muzammil
  • Muhammad Riaz
  • Shabab Nasir
Chapter

Abstract

The most prevalent type of liquid chromatography is ion exchange chromatography. In recent years, the acceptance of ion exchange chromatography has been increased because of its application in agricultural, pharmaceutical, environmental and biotechnology industries. Amino acids are principal components of proteins and their separation from protein hydrolysate or from a mixture of amino acids, is always in focus and ion exchange chromatography finds its application in separation of amino acids. Separation of amino acids by ion exchange chromatography has widespread applications because it has high capacity and it is easy to design. This chapter covers modern applications and basic principles of Ion exchange chromatography in the separation of amino acids.

References

  1. 1.
    Moustafa YM, Morsi RE (2013) Ion exchange chromatography-An overview. In: Column Chromatography. InTechGoogle Scholar
  2. 2.
    Singh C, Sharma C, Kamble P (2014) Amino acid analysis using ion-exchange chromatography: a reviewGoogle Scholar
  3. 3.
    Khan A, Faiz F (2008) Amino acid analysis using ion exchange resins. J Nat Sci Math 48(1–2):1–17Google Scholar
  4. 4.
    Yu H, Ding Y-S, Mou S-F, Jandik P, Cheng J (2002) Simultaneous determination of amino acids and carbohydrates by anion-exchange chromatography with integrated pulsed amperometric detection. J Chromatogr A 966(1):89–97CrossRefGoogle Scholar
  5. 5.
    Bhattacharyya L, Rohrer JS (2012) Applications of ion chromatography in the analysis of pharmaceutical and biological products. Wiley, LondonGoogle Scholar
  6. 6.
    Cascaval D, Oniscu C, Galaction A-I (2001) Selective separation of amino acids by reactive extraction. Biochem Eng J 7(3):171–176CrossRefGoogle Scholar
  7. 7.
    Casella IG, Contursi M (2003) Isocratic ion chromatographic determination of underivatized amino acids by electrochemical detection. Anal Chim Acta 478(2):179–189CrossRefGoogle Scholar
  8. 8.
    Casella IG, Gatta M, Cataldi TR (2000) Amperometric determination of underivatized amino acids at a nickel-modified gold electrode by anion-exchange chromatography. J Chromatogra A 878(1):57–67CrossRefGoogle Scholar
  9. 9.
    Ding Y, Yu H, Mou S (2002) Direct determination of free amino acids and sugars in green tea by anion-exchange chromatography with integrated pulsed amperometric detection. J Chromatogra A 982(2):237–244CrossRefGoogle Scholar
  10. 10.
    Petritis K, Elfakir C, Dreux M (2002) A comparative study of commercial liquid chromatographic detectors for the analysis of underivatized amino acids. J Chromatogra A 961(1):9–21CrossRefGoogle Scholar
  11. 11.
    Small H, Stevens TS, Bauman WC (1975) Novel ion exchange chromatographic method using conductimetric detection. Anal Chem 47(11):1801–1809CrossRefGoogle Scholar
  12. 12.
    Wiesel A, Schmidt-Traub H, Lenz J, Strube J (2003) Modelling gradient elution of bioactive multicomponent systems in non-linear ion-exchange chromatography. J Chromatogra A 1006(1–2):101–120CrossRefGoogle Scholar
  13. 13.
    Cummins PM, Dowling O, O’Connor BF (2011) Ion-exchange chromatography: basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase. In: Protein chromatography. Springer, Berlin, pp 215–228Google Scholar
  14. 14.
    Acikara OzB (2013) Ion-exchange chromatography and its applications. In: Column chromatography. InTechGoogle Scholar
  15. 15.
    Levison PR (2003) Large-scale ion-exchange column chromatography of proteins: comparison of different formats. J Chromatogra B 790(1–2):17–33CrossRefGoogle Scholar
  16. 16.
    Csapo J, Albert C, Loki K, Csapo-Kiss Z (2008) Separation and determination of the amino acids by ion exchange column chromatography applying postcolumn derivatization. Acta Universitatis Sapientiae Alimentaria 1:5–29Google Scholar
  17. 17.
    Csomos E, Simon-Sarkadi L (2002) Characterisation of Tokaj wines based on free amino acids and biogenic amines using ion-exchange chromatography. Chromatographia 56(1):S185–S188CrossRefGoogle Scholar
  18. 18.
    Yu H, Mou S-F (2006) Effect of temperature on the retention of amino acids and carbohydrates in high-performance anion-exchange chromatography. J Chromatogra A 1118(1):118–124CrossRefGoogle Scholar
  19. 19.
    Hong SU, Bruening ML (2006) Separation of amino acid mixtures using multilayer polyelectrolyte nanofiltration membranes. J Membr Sci 280(1–2):1–5Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Khush Bakhat Alia
    • 1
  • Habibullah Nadeem
    • 1
    Email author
  • Ijaz Rasul
    • 1
  • Farrukh Azeem
    • 1
  • Sabir Hussain
    • 2
  • Muhammad Hussnain Siddique
    • 1
  • Saima Muzammil
    • 3
  • Muhammad Riaz
    • 4
  • Shabab Nasir
    • 5
  1. 1.Department of Bioinformatics and BiotechnologyGovernment College UniversityFaisalabadPakistan
  2. 2.Department of Environmental Sciences and EngineeringGovernment College UniversityFaisalabadPakistan
  3. 3.Department of MicrobiologyGovernment College UniversityFaisalabadPakistan
  4. 4.Department of Food SciencesUniversity College of Agriculture, Bahauddin Zakariya UniversityMultanPakistan
  5. 5.Department of ZoologyGovernment College UniversityFaisalabadPakistan

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