Advertisement

Experimental Part

  • Tzu-En LinEmail author
Chapter
Part of the Springer Theses book series (Springer Theses)

Abstract

All the chemicals were of analytical grade and used as received. They are listed in Annex. The phosphate-buffered saline (PBS) (10 mM, pH 7.4) was composed of NaCl 137 mM, KCl 2.7 mM, Na2HPO4 10 mM and KH2PO4 1.8 mM. Washing and blocking buffers of 1% (v/v) BSA were prepared in PBS. The substrate solution for the enzymatic reaction during SECM experiments was made in 0.1 M citrate-phosphate buffer, pH = 5.0, and contained additionally commercial TMB and H2O2 of unknown concentration (not specified by manufacturer).

References

  1. 1.
    C.L. Sun, C.T. Chang, H.H. Lee, J. Zhou, J. Wang, T.K. Sham, W.F. Pong, ACS Nano 5, 7788–7795 (2011)CrossRefGoogle Scholar
  2. 2.
    C.-W. Lin, K.-C. Wei, S. Liao, C.-Y. Huang, C.-L. Sun, P.-J. Wu, Y.-J. Lu, H.-W. Yang, C.-C.M. Ma, Biosens. Bioelectron. 67, 431–437 (2015)CrossRefGoogle Scholar
  3. 3.
    P. Verboven, A. Nemeth, M.K. Abera, E. Bongaers, D. Daelemans, P. Estrade, E. Herremans, M. Hertog, W. Saeys, E. Vanstreels et al., Postharvest Biol. Technol. 78, 123–132 (2013)CrossRefGoogle Scholar
  4. 4.
    D. Post-Beittenmiller, Plant Biol. 47, 405–430 (1996)Google Scholar
  5. 5.
    Z. Ju, W.J. Bramlage, Postharvest Biol. Technol. 16, 107–118 (1999)CrossRefGoogle Scholar
  6. 6.
    A. Lesch, F. Cortés-Salazar, M. Prudent, J. Delobel, S. Rastgar, N. Lion, J.D. Tissot, P. Tacchini, H.H. Girault, J. Electroanal. Chem. 717–718, 61–68 (2014)CrossRefGoogle Scholar
  7. 7.
    C.G. Zoski, Electroanalysis 14, 1041–1051 (2002)CrossRefGoogle Scholar
  8. 8.
    G. Wittstock, T. Asmus, T. Wilhelm, Fresenius J. Anal. Chem. 367, 346–351 (2000)CrossRefGoogle Scholar
  9. 9.
    M. Zhang, A. Becue, M. Prudent, C. Champod, H.H. Girault, Chem. Commun. 3948 (2007)Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Chemistry and Chemical EngineeringÉcole Polytechnique Fédérale de LausanneSionSwitzerland

Personalised recommendations