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A Novel Paper-Based Biosensor for Urinary Phenylalanine Measurement for PKU Therapy Monitoring

  • Maria Anna MessinaEmail author
  • Federica Raudino
  • Agata Fiumara
  • Sabrina Conoci
  • Salvatore PetraliaEmail author
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 539)

Abstract

A novel paper-based biosensors for the measurement of urinary phenylalanine (Phe) was developed and the experimental results here reported. The proposed biosensor is featured by a silicon part integrating temperature sensors and heaters and a polycarbonate ring to form a microchamber. The reagent-on-board format allows a fast and easy self-testing directly from patients. The biosensors is thermally driven by a customized instrument and software. The detection strategy employed is based on the specific reaction of Phenylalanine Ammonia Lyase enzyme to produce ammonia and trans-cinnamic acid from Phenylalanine. The increase of pH value is proportional to the Phe amount and can be monitored by the color changes of a dye solution. The proposed system is suitable to detect the phenylalanine levels in a linear dynamic range concentration from 20 to 3000 μM.

Keywords

Biosensors Phenylalanine Phenylketonuria 

References

  1. 1.
    Kure, S., Hou, D.C., Ohura, T., et al.: Tetrahydrobiopterin-responsive phenylalanine hydroxylase deficiency. J. Pediatr. 135, 375–378 (1999)CrossRefGoogle Scholar
  2. 2.
    Scriver, C.R., Kaufman, S.: Hyperphenylalaninemia: phenylalanine hydroxylase deficiency, 8th edn. Mc Graw-Hill (2001)Google Scholar
  3. 3.
    Fonnesbeck, C.J., McPheeters, M.I., et al.: Estimating the probability of IQ impairment from blood phenylalanine for phenylketonuria patients: a hierarchical meta-analysis. J. Inherited Metab. Dis. 36, 757–766 (2013)CrossRefGoogle Scholar
  4. 4.
    Petralia, S., Conoci, S.: PCR technologies for point of care testing: progress and perspectives. ACS Sens. (2), 876–891 (2017)CrossRefGoogle Scholar
  5. 5.
    Guarnaccia, M., Iemmolo, R., Petralia, S., Conoci, S., Cavallaro, S.: Miniaturized real-time PCR on a Q3 system for rapid KRAS genotyping. Sensors 17(831), 1–9 (2017)Google Scholar
  6. 6.
    Messina, M.A., Meli, C., Conoci, S., Petralia, S.: A facile method for urinary phenylalanine measurement on paper-based lab-on-chip for PKU therapy monitoring. Analyst 142, 4629–4632 (2017)CrossRefGoogle Scholar
  7. 7.
    Petralia, S., Sciuto, E.L., Di Pietro, M.L., Zimbone, M., Grimaldi, M.G., Conoci, S.: Innovative chemical strategy for PCR-free genetic detection of pathogens by an integrated electrochemical biosensor. Analyst 142, 2090–2093 (2017)CrossRefGoogle Scholar
  8. 8.
    Santangelo, M.F., Sciuto, E.L., Busacca, A.C., Petralia, S., Conoci, S., Libertino, S.: Si photomultipliers for bio-sensing applications. IEEE J. Sel. Top. Quantum Electron. 22(3) (2016)CrossRefGoogle Scholar
  9. 9.
    Santangelo, M.F., Sciuto, E.L., Busacca, A.C., Petralia, S., Conoci, S., Libertino, S.: Si PM as miniaturised optical biosensor for DNA-microarray applications. Sens. BioSens. Res. (6) 95–98 (2015)CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Azienda Ospedaliero Universitaria Policlinico Vittorio EmanueleCataniaItaly
  2. 2.STMicroelectronicsCataniaItaly

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