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Portable Optoelectronic System for Monitoring Enzymatic Chemiluminescent Reaction

  • F. Costantini
  • R. M. Tiggelaar
  • R. Salvio
  • M. Nardecchia
  • S. Schlautmann
  • C. Manetti
  • H. J. G. E. Gardeniers
  • D. CaputoEmail author
  • A. Nascetti
  • G. de Cesare
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 539)

Abstract

This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes’ current are enclosed in a small metallic box (\(10 \times 8 \times 15\,\mathrm{cm}^3\)) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 \(\upmu \)M, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors.

Keywords

Photosensors Amorphous silicon Microfluidics Enzymatic reactions Horseradish peroxidase Anodic bonding 

References

  1. 1.
    Luka, G., Ahmadi, A., Najjaran, H., Alocilja, E., de Rosa, M., Wolthers, K., Malki, A., Aziz, H., Althani, A., Hoorfar, M.: Microfluidics integrated biosensors: a leading technology towards lab-on-a-chip and sensing applications. Sensors 15(12), 30011–30031 (2015)CrossRefGoogle Scholar
  2. 2.
    Costantini, F., Sberna, C., Petrucci, G., Manetti, C., de Cesare, G., Nascetti, A., Caputo, D.: Lab-on-chip system combining a microfluidic-ELISA with an array of amorphous silicon photosensors for the detection of celiac disease epitopes. Sens. Bio-Sens. Res. 6, 51–58 (2015)CrossRefGoogle Scholar
  3. 3.
    Costantini, F., Sberna, C., Petrucci, G., Reverberi, M., Domenici, F., Fanelli, C., Manetti, C., de Cesare, G., DeRosa, M., Nascetti, A., Caputo, D.: Aptamer-based sandwich assay for on chip detection of Ochratoxin a by an array of amorphous silicon photosensors. Sens. Actuators B: Chem. 230, 31–39 (2016)CrossRefGoogle Scholar
  4. 4.
    Costantini, F., Tiggelaar, R., Sennato, S., Mura, F., Schlautmann, S., Bordi, F., Gardeniers, H., Manetti, C.: Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip. Analyst 138(17), 5019–5024 (2013)CrossRefGoogle Scholar
  5. 5.
    Zhang, Z., Zhang, S., Zhang, X.: Recent developments and applications of chemiluminescence sensors. Anal. Chim. Acta 541(1–2), 37–47 (2005)CrossRefGoogle Scholar
  6. 6.
    Hofmann, O., Miller, P., Sullivan, P., Jones, T.S., Bradley, D.D.: Thin-film organic photodiodes as integrated detectors for microscale chemiluminescence assays. Sens. Actuators B: Chem. 106(2), 878–884 (2005)CrossRefGoogle Scholar
  7. 7.
    Jorgensen, A.M., Mogensen, K.B., Kutter, J.P., Geschke, O.: A biochemical microdevice with an integrated chemiluminescence detector. Sens. Actuators B: Chem. 90(1–3), 15–21 (2003)CrossRefGoogle Scholar
  8. 8.
    Caputo, D., de Cesare, G., Scipinotti, R., Stasio, N., Costantini, F., Manetti, C., Nascetti, A.: On-chip diagnosis of celiac disease by an amorphous silicon chemiluminescence detector. In: Sensors and Microsystems, pp. 183–187. Springer, Cham. (2014)Google Scholar
  9. 9.
    Costantini, F., Benetti, E.M., Tiggelaar, R.M., Gardeniers, H.J., Reinhoudt, D.N., Huskens, J., Vancso, G.J., Verboom, W.: A Brush?Gel/Metal?Nanoparticle hybrid film as an efficient supported catalyst in glass microreactors. Chem.-A Eur. J. 16(41), 12406–12411 (2010)CrossRefGoogle Scholar
  10. 10.
    Tahirovic, A., Copra, A., Omanovic-Miklicanin, E., Kalcher, K.: A chemiluminescence sensor for the determination of hydrogen peroxide. Talanta 72(4), 1378–1385 (2007)CrossRefGoogle Scholar
  11. 11.
    Kovarik, M.L., Gach, P.C., Ornoff, D.M., Wang, Y., Balowski, J., Farrag, L., Allbritton, N.L.: Micro total analysis systems for cell biology and biochemical assays. Anal. Chem. 84(2), 516–540 (2011)CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • F. Costantini
    • 1
    • 2
  • R. M. Tiggelaar
    • 3
  • R. Salvio
    • 2
  • M. Nardecchia
    • 1
  • S. Schlautmann
    • 3
  • C. Manetti
    • 4
  • H. J. G. E. Gardeniers
    • 3
  • D. Caputo
    • 5
    Email author
  • A. Nascetti
    • 1
  • G. de Cesare
    • 5
  1. 1.School of Aerospace EngineeringSapienza University of RomeRomeItaly
  2. 2.Department of ChemistrySapienza University of RomeRomeItaly
  3. 3.MESA+ Institute for Nanotechnology, University of TwenteEnschedeThe Netherlands
  4. 4.Department of Environmental BiologySapienza University of RomeRomeItaly
  5. 5.Department of Information Engineering, Electronics and TelecommunicationsSapienza University of RomeRomeItaly

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