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FITC-Labelled Clone from Phage Display for Direct Detection of Leukemia Cells in Blood

  • Domenico FrancoEmail author
  • Laura M. De Plano
  • Maria Giovanna Rizzo
  • Sara Crea
  • Enza Fazio
  • Martina Bonsignore
  • Fortunato Neri
  • Alessandro Allegra
  • Caterina Musolino
  • Guido Ferlazzo
  • Sebastiano Trusso
  • Salvatore P. P. Guglielmino
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 539)

Abstract

Discovery of new markers for the identification and discrimination of cell types is one of the principal objectives in cancer diagnostics. In the last years, many researchers used phage-display technology in vitro and in vivo to obtain random peptide probes able to bind towards cancer targets to be used in diagnostic systems and new targeted drug. In this work, we proposed a Single Drop Biosensor based on phage-labelled probes to detect leukaemia cells in blood from patients affected by chronic lymphocytic leukaemia (CLL). Results show that phage-labelled probes were able to recognize lymphocytes and lymphoblastic cells both in leukemic peripheral blood mononuclear cells and in whole blood from patients affected by CLL. The “proof of concept” proposed, using the phage labelled as bio-probe, could be an alternative way to produce new biosensor for monitoring of chronic pathology. Furthermore the results may have translational relevance for identification and exploring of new ligands directed against cancer hematological cells.

Keywords

Phage-display Leukemia Fluorescence imaging 

Notes

Acknowledgements

This work was supported by Associazione pro Bambini e Adulti Leucemici (A.B.A.L.) onlus Messina (Italy) (http://www.abalmessina.it).

Phage-display libraries were a kind gift of Prof. Franco Felici.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Domenico Franco
    • 1
    Email author
  • Laura M. De Plano
    • 2
  • Maria Giovanna Rizzo
    • 2
  • Sara Crea
    • 2
  • Enza Fazio
    • 1
  • Martina Bonsignore
    • 1
  • Fortunato Neri
    • 1
  • Alessandro Allegra
    • 3
  • Caterina Musolino
    • 3
  • Guido Ferlazzo
    • 4
  • Sebastiano Trusso
    • 5
  • Salvatore P. P. Guglielmino
    • 2
  1. 1.Department of Mathematical and Computational Sciences, Physical Science and Earth ScienceUniversity of MessinaMessinaItaly
  2. 2.Department of Chemical Sciences, Biological, Pharmaceutical and EnvironmentalUniversity of MessinaMessinaItaly
  3. 3.Division of Hematology, Department of Human Pathology in Adulthood and Childhood “Gaetano Barresi”University of MessinaMessinaItaly
  4. 4.Laboratory of Immunology and Biotherapy, Department of Human PathologyUniversity of MessinaMessinaItaly
  5. 5.Institute of Chemical-Physical Processes (IPCF)-CNRMessinaItaly

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